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Protein

Probable replication factor A 73 kDa subunit

Gene

rpa-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi236 – 32691OBAdd
BLAST
Zinc fingeri518 – 53922C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • enzyme binding Source: WormBase
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-110312. Translesion synthesis by REV1.
R-CEL-110320. Translesion Synthesis by POLH.
R-CEL-176187. Activation of ATR in response to replication stress.
R-CEL-5655862. Translesion synthesis by POLK.
R-CEL-5696395. Formation of Incision Complex in GG-NER.
R-CEL-5696400. Dual Incision in GG-NER.
R-CEL-6782135. Dual incision in TC-NER.
R-CEL-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable replication factor A 73 kDa subunit
Alternative name(s):
RP-A p73
Replication factor A protein 1
Short name:
RF-A protein 1
Gene namesi
Name:rpa-1
ORF Names:F18A1.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF18A1.5; CE04405; WBGene00017546; rpa-1.

Subcellular locationi

GO - Cellular componenti

  • DNA replication factor A complex Source: UniProtKB
  • nucleoplasm Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Probable replication factor A 73 kDa subunitPRO_0000097257Add
BLAST

Proteomic databases

EPDiQ19537.
PaxDbiQ19537.
PRIDEiQ19537.

Expressioni

Gene expression databases

BgeeiWBGene00017546.

Interactioni

Subunit structurei

Component of the heterotrimeric canonical replication protein A complex (RPA).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
rpa-2Q95Y973EBI-325415,EBI-325404

GO - Molecular functioni

  • enzyme binding Source: WormBase

Protein-protein interaction databases

BioGridi39572. 3 interactions.
DIPiDIP-26609N.
IntActiQ19537. 2 interactions.
MINTiMINT-1119512.
STRINGi6239.F18A1.5.

Structurei

3D structure databases

ProteinModelPortaliQ19537.
SMRiQ19537. Positions 221-627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 OB DNA-binding domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri518 – 53922C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0851. Eukaryota.
COG1599. LUCA.
GeneTreeiENSGT00390000012403.
HOGENOMiHOG000154096.
InParanoidiQ19537.
KOiK07466.
OMAiGETWISS.
OrthoDBiEOG091G02VJ.
PhylomeDBiQ19537.

Family and domain databases

Gene3Di2.40.50.140. 4 hits.
InterProiIPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR013955. Rep_factor-A_C.
IPR031657. REPA_OB_2.
IPR004591. Rfa1.
[Graphical view]
PfamiPF08646. Rep_fac-A_C. 1 hit.
PF16900. REPA_OB_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 3 hits.
TIGRFAMsiTIGR00617. rpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q19537-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIHINHDV FNKYHTNGKL RLTTGYVQEA IENNGYPGHD GIVQVLKGKV
60 70 80 90 100
EQGEQLGHAF TFRIRISDGV FQYNALMSAD IDDQIKREVE HLVEGTIIAL
110 120 130 140 150
TKFEIYDQGE GAKNCFLIKG YKILSRYHQV LTSPEVKPRS HSGKPDEHKG
160 170 180 190 200
YRPNIIIEDV WPEAEGMAAD YQENMANPPA AKAPKREFGE EASYNRAAAP
210 220 230 240 250
EATRARAVPP PARRTASNTE RGVMPIAMVT PYVSNFKIHG MVSRKEEIRT
260 270 280 290 300
FPAKNTKVFN FEITDSNGDT IRCTAFNEVA ESLYTTITEN LSYYLSGGSV
310 320 330 340 350
KQANKKFNNT GHDYEITLRS DSIIEAGGEL LAAPKLILKR VKLGEIAGYA
360 370 380 390 400
GQLIDVLVVV EKMDPEATEF TSKAGKSLIK REMELIDESG ALVRLTLWGD
410 420 430 440 450
EATKALVDDY VQKVIAFKGV IPREFNGGFS LGTGSATRII SVPEIAGVSE
460 470 480 490 500
LYDWYANVKP TTEVKMMSQA AGGSNEAPRT IAGLQEMQFG KDSDKGDYAT
510 520 530 540 550
VKAMITRVNP TNALYRGCAS EGCQKKLVGE NGDYRCEKCN KNMNKFKWLY
560 570 580 590 600
MMQFELSDET GQVYVTAFGD SAAKIVGKSA AELGELHDES PDEYNAIFER
610 620 630 640 650
LQFVPKMWRL RCKMDSYNEE VRQKMTVYGV DDVNQDKYIE NLKQMIEQMQ

QMSDY
Length:655
Mass (Da):73,202
Last modified:November 1, 1996 - v1
Checksum:i44E41E7B16E2FB42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080212 Genomic DNA. Translation: CCD62042.1.
PIRiT34219.
RefSeqiNP_495606.1. NM_063205.4.
UniGeneiCel.34208.

Genome annotation databases

EnsemblMetazoaiF18A1.5; F18A1.5; WBGene00017546.
GeneIDi174238.
KEGGicel:CELE_F18A1.5.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080212 Genomic DNA. Translation: CCD62042.1.
PIRiT34219.
RefSeqiNP_495606.1. NM_063205.4.
UniGeneiCel.34208.

3D structure databases

ProteinModelPortaliQ19537.
SMRiQ19537. Positions 221-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39572. 3 interactions.
DIPiDIP-26609N.
IntActiQ19537. 2 interactions.
MINTiMINT-1119512.
STRINGi6239.F18A1.5.

Proteomic databases

EPDiQ19537.
PaxDbiQ19537.
PRIDEiQ19537.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF18A1.5; F18A1.5; WBGene00017546.
GeneIDi174238.
KEGGicel:CELE_F18A1.5.

Organism-specific databases

CTDi174238.
WormBaseiF18A1.5; CE04405; WBGene00017546; rpa-1.

Phylogenomic databases

eggNOGiKOG0851. Eukaryota.
COG1599. LUCA.
GeneTreeiENSGT00390000012403.
HOGENOMiHOG000154096.
InParanoidiQ19537.
KOiK07466.
OMAiGETWISS.
OrthoDBiEOG091G02VJ.
PhylomeDBiQ19537.

Enzyme and pathway databases

ReactomeiR-CEL-110312. Translesion synthesis by REV1.
R-CEL-110320. Translesion Synthesis by POLH.
R-CEL-176187. Activation of ATR in response to replication stress.
R-CEL-5655862. Translesion synthesis by POLK.
R-CEL-5696395. Formation of Incision Complex in GG-NER.
R-CEL-5696400. Dual Incision in GG-NER.
R-CEL-6782135. Dual incision in TC-NER.
R-CEL-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Miscellaneous databases

PROiQ19537.

Gene expression databases

BgeeiWBGene00017546.

Family and domain databases

Gene3Di2.40.50.140. 4 hits.
InterProiIPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR013955. Rep_factor-A_C.
IPR031657. REPA_OB_2.
IPR004591. Rfa1.
[Graphical view]
PfamiPF08646. Rep_fac-A_C. 1 hit.
PF16900. REPA_OB_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 3 hits.
TIGRFAMsiTIGR00617. rpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFA1_CAEEL
AccessioniPrimary (citable) accession number: Q19537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.