Q194Q8 (Q194Q8_STRAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Ketoreductase EMBL CAK50780.1

Gene names

Name:

mtmTI EMBL CAK50780.1

Organism

Streptomyces argillaceus EMBL CAK50780.1

Taxonomic identifier

41951 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Gene Ontology (GO)
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q194Q8 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 5C4C799964F5BDE9	FASTA	254	25,954
	10 20 30 40 50 60 MTTHHSTAPA LTGRTALVTG ATRGVGFAIA RKLCARGADV LLDYAEDEAN AARAVGALAG 70 80 90 100 110 120 LPGRAAAVRA DVTVDFDALC DEVGQRFGHL DIFVHNTSYF RPAPTLATTP EQVDRSLAVA 130 140 150 160 170 180 LRPLLTGASR LAGLMAGRPG RIVAVSSTGA HRVVPRYAGA GMAKAALESL VRYLAVELAG 190 200 210 220 230 240 RGIAVNAVAA AKVDKGDGTV PAEVAEAITA RTPGGRLTRP EDVADAVSLL CTDEARWLQG 250 QVVTVDGGLG LVAG

References

[1]	"Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin." Lombo F., Blanco G., Fernandez E., Mendez C., Salas J.A. Gene 172:87-91(1996) [PubMed: 8654997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[2]	"An ABC transporter is essential for resistance to the antitumor agent mithramycin in the producer Streptomyces argillaceus." Fernandez E., Lombo F., Mendez C., Salas J.A. Mol. Gen. Genet. 251:692-698(1996) [PubMed: 8757400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[3]	"Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin." Lombo F., Siems K., Brana A.F., Mendez C., Bindseil K., Salas J.A. J. Bacteriol. 179:3354-3357(1997) [PubMed: 9150235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[4]	"Identification of two genes from Streptomyces argillaceus encoding glycosyltransferases involved in transfer of a disaccharide during biosynthesis of the antitumor drug mithramycin." Fernandez E., Weissbach U., Sanchez Reillo C., Brana A.F., Mendez C., Rohr J., Salas J.A. J. Bacteriol. 180:4929-4937(1998) [PubMed: 9733697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[5]	"Analysis of two chromosomal regions adjacent to genes for a type II polyketide synthase involved in the biosynthesis of the antitumor polyketide mithramycin in Streptomyces argillaceus." Prado L., Lombo F., Brana A.F., Mendez C., Rohr J., Salas J.A. Mol. Gen. Genet. 261:216-225(1999) [PubMed: 10102355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[6]	"Oxidative cleavage of premithramycin B is one of the last steps in the biosynthesis of the antitumor drug mithramycin." Prado L., Fernandez E., Weissbach U., Blanco G., Quiros L.M., Brana A.F., Mendez C., Rohr J., Salas J.A. Chem. Biol. 6:19-30(1999) [PubMed: 9889148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[7]	"Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus." Blanco G., Fernandez E., Fernandez M.J., Brana A.F., Weissbach U., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 262:991-1000(2000) [PubMed: 10660060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[8]	"The mtmVUC genes of the mithramycin gene cluster in Streptomyces argillaceus are involved in the biosynthesis of the sugar moieties." Gonzalez A., Remsing L.L., Lombo F., Fernandez M.J., Prado L., Brana A.F., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 264:827-835(2001) [PubMed: 11254130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[9]	"Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces argillaceus through combinatorial biosynthesis." Remsing L.L., Gonzalez A.M., Nur-e-Alam M., Fernandez-Lozano M.J., Brana A.F., Rix U., Oliveira M.A., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 125:5745-5753(2003) [PubMed: 12733914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[10]	"The structure of mithramycin reinvestigated." Wohlert S.E., Kunzel E., Machinek R., Mendez C., Salas J.A., Rohr J. J. Nat. Prod. 62:119-121(1999) [PubMed: 9917296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[11]	"Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway." Remsing L.L., Garcia-Bernardo J., Gonzalez A., Kunzel E., Rix U., Brana A.F., Bearden D.W., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 124:1606-1614(2002) [PubMed: 11853433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[12]	"Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin by Streptomyces argillaceus." Lozano M.J., Remsing L.L., Quiros L.M., Brana A.F., Fernandez E., Sanchez C., Mendez C., Rohr J., Salas J.A. J. Biol. Chem. 275:3065-3074(2000) [PubMed: 10652287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[13]	"Insertional inactivation of mtrX and mtrY genes from the mithramycin gene cluster affects production and growth of the producer organism Streptomyces argillaceus." Garcia-Bernardo J., Brana A.F., Mendez C., Salas J.A. FEMS Microbiol. Lett. 186:61-65(2000) [PubMed: 10779713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[14]	"Rationally designed glycosylated premithramycins: hybrid aromatic polyketides using genes from three different biosynthetic pathways." Trefzer A., Blanco G., Remsing L., Kunzel E., Rix U., Lipata F., Brana A.F., Mendez C., Rohr J., Bechthold A., Salas J.A. J. Am. Chem. Soc. 124:6056-6062(2002) [PubMed: 12022840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[15]	"Purification and characterization of a monooxygenase involved in the biosynthetic pathway of the antitumor drug mithramycin." Rodriguez D., Quiros L.M., Brana A.F., Salas J.A. J. Bacteriol. 185:3962-3965(2003) [PubMed: 12813091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[16]	"Deciphering the biosynthetic origin of the aglycone of the aureolic acid group of anti-tumor agents." Blanco G., Fu H., Mendez C., Khosla C., Salas J.A. Chem. Biol. 3:193-196(1996) [PubMed: 8807845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[17]	"MtmMII-mediated C-methylation during biosynthesis of the antitumor drug mithramycin is essential for biological activity and DNA-drug interaction." Rodriguez D., Quiros L.M., Salas J.A. J. Biol. Chem. 279:8149-8158(2004) [PubMed: 14660589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[18]	"Folding of the polyketide chain is not dictated by minimal polyketide synthase in the biosynthesis of mithramycin and anthracycline." Kantola J., Blanco G., Hautala A., Kunnari T., Hakala J., Mendez C., Ylihonko K., Mantsala P., Salas J. Chem. Biol. 4:751-755(1997) [PubMed: 9375253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[19]	"Elucidation of the glycosylation sequence of mithramycin biosynthesis: isolation of 3A-deolivosylpremithramycin B and its conversion to premithramycin B by glycosyltransferase MtmGII." Nur-e-Alam M., Mendez C., Salas J.A., Rohr J. Chembiochem 6:632-636(2005) [PubMed: 15712316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[20]	"The Novel Hybrid Antitumor Compound Premithramycinone H Provides Indirect Evidence for a Tricyclic Intermediate of the Biosynthesis of the Aureolic Acid Antibiotic Mithramycin." Lombo F., Kunzel E., Prado L., Brana A.F., Bindseil K.U., Frevert J., Bearden D., Mendez C., Salas J.A., Rohr J. Angew. Chem. Int. Ed. 39:796-799(2000) [PubMed: 10760873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.
[21]	Lombo F. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50780.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89899 Genomic DNA. Translation: CAK50780.1.
PIR	S58176.
3D structure databases
ProteinModelPortal	Q194Q8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

Q194Q8_STRAA

Accession

Primary (citable) accession number: Q194Q8

Entry history

Integrated into UniProtKB/TrEMBL:	July 11, 2006
Last sequence update:	July 11, 2006
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)