Q194Q4 (Q194Q4_STRAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
D-mycarose 3-C-methyltransferase EMBL CAK50784.1

Gene names

Name:

mtmC EMBL CAK50784.1

Organism

Streptomyces argillaceus EMBL CAK50784.1

Taxonomic identifier

41951 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Molecular function	Methyltransferase EMBL CAK50784.1 Transferase
Gene Ontology (GO)
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q194Q4 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: B0F7DDCC71882844	FASTA	421	45,811
	10 20 30 40 50 60 MDIYGTARAV TTCPMCGAQD WQEVVDFGPV PLADSFLEPA ASYDDEPRYP LAVVSCASCR 70 80 90 100 110 120 LMSLTHVVDP EVLYRTYPYT TSDSETIKKH MGHVVAGVRP SASASPEGSF VLEIGSNTGS 130 140 150 160 170 180 QLKAFQNAGM RTLGIDPARN IAAVANERGI ETLPEFFSVD TAALVKKTHG TPQLVLGRHV 190 200 210 220 230 240 FAHIDDVSAV AEGVRDLLGP DSLFAIEVPY LVDMLERNEF DTIYHEHLSY IGVGSLVALF 250 260 270 280 290 300 RRHGLRVVDV ERLAVHGGSI LVFVGLDEGT RATAPVVEEL IALEKERGLY EDATYERFAR 310 320 330 340 350 360 HVAEITAELT SMVRSLRAEG KRIAGYGAPA KGNTLLNVCG LTADDLEFCC DTTEFKQGLV 370 380 390 400 410 420 LPGTHIPVRS PEYAKTQAID YYLLLAWNYG EEILAKEGPF LADGGRFILP NPRPSIVPPG E

References

[1]	"Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin." Lombo F., Blanco G., Fernandez E., Mendez C., Salas J.A. Gene 172:87-91(1996) [PubMed: 8654997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[2]	"An ABC transporter is essential for resistance to the antitumor agent mithramycin in the producer Streptomyces argillaceus." Fernandez E., Lombo F., Mendez C., Salas J.A. Mol. Gen. Genet. 251:692-698(1996) [PubMed: 8757400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[3]	"Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin." Lombo F., Siems K., Brana A.F., Mendez C., Bindseil K., Salas J.A. J. Bacteriol. 179:3354-3357(1997) [PubMed: 9150235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[4]	"Identification of two genes from Streptomyces argillaceus encoding glycosyltransferases involved in transfer of a disaccharide during biosynthesis of the antitumor drug mithramycin." Fernandez E., Weissbach U., Sanchez Reillo C., Brana A.F., Mendez C., Rohr J., Salas J.A. J. Bacteriol. 180:4929-4937(1998) [PubMed: 9733697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[5]	"Analysis of two chromosomal regions adjacent to genes for a type II polyketide synthase involved in the biosynthesis of the antitumor polyketide mithramycin in Streptomyces argillaceus." Prado L., Lombo F., Brana A.F., Mendez C., Rohr J., Salas J.A. Mol. Gen. Genet. 261:216-225(1999) [PubMed: 10102355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[6]	"Oxidative cleavage of premithramycin B is one of the last steps in the biosynthesis of the antitumor drug mithramycin." Prado L., Fernandez E., Weissbach U., Blanco G., Quiros L.M., Brana A.F., Mendez C., Rohr J., Salas J.A. Chem. Biol. 6:19-30(1999) [PubMed: 9889148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[7]	"Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus." Blanco G., Fernandez E., Fernandez M.J., Brana A.F., Weissbach U., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 262:991-1000(2000) [PubMed: 10660060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[8]	"The mtmVUC genes of the mithramycin gene cluster in Streptomyces argillaceus are involved in the biosynthesis of the sugar moieties." Gonzalez A., Remsing L.L., Lombo F., Fernandez M.J., Prado L., Brana A.F., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 264:827-835(2001) [PubMed: 11254130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[9]	"Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces argillaceus through combinatorial biosynthesis." Remsing L.L., Gonzalez A.M., Nur-e-Alam M., Fernandez-Lozano M.J., Brana A.F., Rix U., Oliveira M.A., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 125:5745-5753(2003) [PubMed: 12733914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[10]	"The structure of mithramycin reinvestigated." Wohlert S.E., Kunzel E., Machinek R., Mendez C., Salas J.A., Rohr J. J. Nat. Prod. 62:119-121(1999) [PubMed: 9917296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[11]	"Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway." Remsing L.L., Garcia-Bernardo J., Gonzalez A., Kunzel E., Rix U., Brana A.F., Bearden D.W., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 124:1606-1614(2002) [PubMed: 11853433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[12]	"Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin by Streptomyces argillaceus." Lozano M.J., Remsing L.L., Quiros L.M., Brana A.F., Fernandez E., Sanchez C., Mendez C., Rohr J., Salas J.A. J. Biol. Chem. 275:3065-3074(2000) [PubMed: 10652287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[13]	"Insertional inactivation of mtrX and mtrY genes from the mithramycin gene cluster affects production and growth of the producer organism Streptomyces argillaceus." Garcia-Bernardo J., Brana A.F., Mendez C., Salas J.A. FEMS Microbiol. Lett. 186:61-65(2000) [PubMed: 10779713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[14]	"Rationally designed glycosylated premithramycins: hybrid aromatic polyketides using genes from three different biosynthetic pathways." Trefzer A., Blanco G., Remsing L., Kunzel E., Rix U., Lipata F., Brana A.F., Mendez C., Rohr J., Bechthold A., Salas J.A. J. Am. Chem. Soc. 124:6056-6062(2002) [PubMed: 12022840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[15]	"Purification and characterization of a monooxygenase involved in the biosynthetic pathway of the antitumor drug mithramycin." Rodriguez D., Quiros L.M., Brana A.F., Salas J.A. J. Bacteriol. 185:3962-3965(2003) [PubMed: 12813091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[16]	"Deciphering the biosynthetic origin of the aglycone of the aureolic acid group of anti-tumor agents." Blanco G., Fu H., Mendez C., Khosla C., Salas J.A. Chem. Biol. 3:193-196(1996) [PubMed: 8807845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[17]	"MtmMII-mediated C-methylation during biosynthesis of the antitumor drug mithramycin is essential for biological activity and DNA-drug interaction." Rodriguez D., Quiros L.M., Salas J.A. J. Biol. Chem. 279:8149-8158(2004) [PubMed: 14660589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[18]	"Folding of the polyketide chain is not dictated by minimal polyketide synthase in the biosynthesis of mithramycin and anthracycline." Kantola J., Blanco G., Hautala A., Kunnari T., Hakala J., Mendez C., Ylihonko K., Mantsala P., Salas J. Chem. Biol. 4:751-755(1997) [PubMed: 9375253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[19]	"Elucidation of the glycosylation sequence of mithramycin biosynthesis: isolation of 3A-deolivosylpremithramycin B and its conversion to premithramycin B by glycosyltransferase MtmGII." Nur-e-Alam M., Mendez C., Salas J.A., Rohr J. Chembiochem 6:632-636(2005) [PubMed: 15712316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[20]	"The Novel Hybrid Antitumor Compound Premithramycinone H Provides Indirect Evidence for a Tricyclic Intermediate of the Biosynthesis of the Aureolic Acid Antibiotic Mithramycin." Lombo F., Kunzel E., Prado L., Brana A.F., Bindseil K.U., Frevert J., Bearden D., Mendez C., Salas J.A., Rohr J. Angew. Chem. Int. Ed. 39:796-799(2000) [PubMed: 10760873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.
[21]	Lombo F. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50784.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89899 Genomic DNA. Translation: CAK50784.1.
3D structure databases
ProteinModelPortal	Q194Q4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013630. Methyltransf_13. IPR013691. MeTrfase_14. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08421. Methyltransf_13. 1 hit. PF08484. Methyltransf_14. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q194Q4_STRAA

Accession

Primary (citable) accession number: Q194Q4

Entry history

Integrated into UniProtKB/TrEMBL:	July 11, 2006
Last sequence update:	July 11, 2006
Last modified:	October 5, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)