Q194P7 (Q194P7_STRAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
O-methyltransferase EMBL CAK50791.1

Gene names

Name:

mtmMI EMBL CAK50791.1

Organism

Streptomyces argillaceus EMBL CAK50791.1

Taxonomic identifier

41951 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Molecular function	Methyltransferase EMBL CAK50791.1 Transferase
Gene Ontology (GO)
Molecular function	O-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q194P7 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 792FAE2B25F2C30F	FASTA	345	37,864
	10 20 30 40 50 60 MTNTPAAAPS PADAREILQL NVAYTRARVL HTAMELGLFE YLSAGPRTEA EIREHGRLHS 70 80 90 100 110 120 HLVRDWLDAL VGLGLLTADG GTYANTPRAE HFLVPGHPHY IGGAVLQHGR VHYELWNRFA 130 140 150 160 170 180 DALRDGKATS GKDITAGAVV EEQPDLDRAR RFLDHMDTFN QFVVAELSKA LDWSRWKTFV 190 200 210 220 230 240 DVGGARGNVA AQLVLDHPDL KGSVFDVPGV EPLFDEHMAA RGTTGRVDFV GGDFFTDALP 250 260 270 280 290 300 AADVVLFGHV LHDHPEESRK RLLGQAFRAL PPGGTLLVYD AMLDDRSEAG AYLQSLVCSV 310 320 330 340 ILDGGSEYRV RDITAWAEET GFTVEQVVPL DTMTHDRLFV ARKPG

References

[1]	"Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin." Lombo F., Blanco G., Fernandez E., Mendez C., Salas J.A. Gene 172:87-91(1996) [PubMed: 8654997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[2]	"An ABC transporter is essential for resistance to the antitumor agent mithramycin in the producer Streptomyces argillaceus." Fernandez E., Lombo F., Mendez C., Salas J.A. Mol. Gen. Genet. 251:692-698(1996) [PubMed: 8757400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[3]	"Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin." Lombo F., Siems K., Brana A.F., Mendez C., Bindseil K., Salas J.A. J. Bacteriol. 179:3354-3357(1997) [PubMed: 9150235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[4]	"Identification of two genes from Streptomyces argillaceus encoding glycosyltransferases involved in transfer of a disaccharide during biosynthesis of the antitumor drug mithramycin." Fernandez E., Weissbach U., Sanchez Reillo C., Brana A.F., Mendez C., Rohr J., Salas J.A. J. Bacteriol. 180:4929-4937(1998) [PubMed: 9733697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[5]	"Analysis of two chromosomal regions adjacent to genes for a type II polyketide synthase involved in the biosynthesis of the antitumor polyketide mithramycin in Streptomyces argillaceus." Prado L., Lombo F., Brana A.F., Mendez C., Rohr J., Salas J.A. Mol. Gen. Genet. 261:216-225(1999) [PubMed: 10102355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[6]	"Oxidative cleavage of premithramycin B is one of the last steps in the biosynthesis of the antitumor drug mithramycin." Prado L., Fernandez E., Weissbach U., Blanco G., Quiros L.M., Brana A.F., Mendez C., Rohr J., Salas J.A. Chem. Biol. 6:19-30(1999) [PubMed: 9889148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[7]	"Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus." Blanco G., Fernandez E., Fernandez M.J., Brana A.F., Weissbach U., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 262:991-1000(2000) [PubMed: 10660060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[8]	"The mtmVUC genes of the mithramycin gene cluster in Streptomyces argillaceus are involved in the biosynthesis of the sugar moieties." Gonzalez A., Remsing L.L., Lombo F., Fernandez M.J., Prado L., Brana A.F., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 264:827-835(2001) [PubMed: 11254130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[9]	"Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces argillaceus through combinatorial biosynthesis." Remsing L.L., Gonzalez A.M., Nur-e-Alam M., Fernandez-Lozano M.J., Brana A.F., Rix U., Oliveira M.A., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 125:5745-5753(2003) [PubMed: 12733914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[10]	"The structure of mithramycin reinvestigated." Wohlert S.E., Kunzel E., Machinek R., Mendez C., Salas J.A., Rohr J. J. Nat. Prod. 62:119-121(1999) [PubMed: 9917296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[11]	"Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway." Remsing L.L., Garcia-Bernardo J., Gonzalez A., Kunzel E., Rix U., Brana A.F., Bearden D.W., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 124:1606-1614(2002) [PubMed: 11853433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[12]	"Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin by Streptomyces argillaceus." Lozano M.J., Remsing L.L., Quiros L.M., Brana A.F., Fernandez E., Sanchez C., Mendez C., Rohr J., Salas J.A. J. Biol. Chem. 275:3065-3074(2000) [PubMed: 10652287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[13]	"Insertional inactivation of mtrX and mtrY genes from the mithramycin gene cluster affects production and growth of the producer organism Streptomyces argillaceus." Garcia-Bernardo J., Brana A.F., Mendez C., Salas J.A. FEMS Microbiol. Lett. 186:61-65(2000) [PubMed: 10779713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[14]	"Rationally designed glycosylated premithramycins: hybrid aromatic polyketides using genes from three different biosynthetic pathways." Trefzer A., Blanco G., Remsing L., Kunzel E., Rix U., Lipata F., Brana A.F., Mendez C., Rohr J., Bechthold A., Salas J.A. J. Am. Chem. Soc. 124:6056-6062(2002) [PubMed: 12022840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[15]	"Purification and characterization of a monooxygenase involved in the biosynthetic pathway of the antitumor drug mithramycin." Rodriguez D., Quiros L.M., Brana A.F., Salas J.A. J. Bacteriol. 185:3962-3965(2003) [PubMed: 12813091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[16]	"Deciphering the biosynthetic origin of the aglycone of the aureolic acid group of anti-tumor agents." Blanco G., Fu H., Mendez C., Khosla C., Salas J.A. Chem. Biol. 3:193-196(1996) [PubMed: 8807845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[17]	"MtmMII-mediated C-methylation during biosynthesis of the antitumor drug mithramycin is essential for biological activity and DNA-drug interaction." Rodriguez D., Quiros L.M., Salas J.A. J. Biol. Chem. 279:8149-8158(2004) [PubMed: 14660589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[18]	"Folding of the polyketide chain is not dictated by minimal polyketide synthase in the biosynthesis of mithramycin and anthracycline." Kantola J., Blanco G., Hautala A., Kunnari T., Hakala J., Mendez C., Ylihonko K., Mantsala P., Salas J. Chem. Biol. 4:751-755(1997) [PubMed: 9375253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[19]	"Elucidation of the glycosylation sequence of mithramycin biosynthesis: isolation of 3A-deolivosylpremithramycin B and its conversion to premithramycin B by glycosyltransferase MtmGII." Nur-e-Alam M., Mendez C., Salas J.A., Rohr J. Chembiochem 6:632-636(2005) [PubMed: 15712316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[20]	"The Novel Hybrid Antitumor Compound Premithramycinone H Provides Indirect Evidence for a Tricyclic Intermediate of the Biosynthesis of the Aureolic Acid Antibiotic Mithramycin." Lombo F., Kunzel E., Prado L., Brana A.F., Bindseil K.U., Frevert J., Bearden D., Mendez C., Salas J.A., Rohr J. Angew. Chem. Int. Ed. 39:796-799(2000) [PubMed: 10760873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.
[21]	Lombo F. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAK50791.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89899 Genomic DNA. Translation: CAK50791.1.
3D structure databases
ProteinModelPortal	Q194P7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q194P7_STRAA

Accession

Primary (citable) accession number: Q194P7

Entry history

Integrated into UniProtKB/TrEMBL:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	September 21, 2011
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)