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Q19426 (GCS1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannosyl-oligosaccharide glucosidase
EC=3.2.1.106
Alternative name(s):
Processing A-glucosidase I
Gene names
ORF Names:F13H10.4
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length796 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically By similarity.

Catalytic activity

Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 63 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processoligosaccharide metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmannosyl-oligosaccharide glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q19426-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: Q19426-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 796796Probable mannosyl-oligosaccharide glucosidase
PRO_0000057715

Regions

Topological domain1 – 4646Cytoplasmic Potential
Transmembrane47 – 6721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain68 – 796729Lumenal Potential
Region70 – 13061Required for endoplasmic reticulum targeting By similarity
Motif2 – 87Endoplasmic reticulum targeting

Amino acid modifications

Glycosylation1531N-linked (GlcNAc...) Ref.2
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation5171N-linked (GlcNAc...) Ref.3

Natural variations

Alternative sequence1 – 77Missing in isoform b.
VSP_021635

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: A9DB69788CBB1F09

FASTA79692,646
        10         20         30         40         50         60 
MHREHEEMHQ PSRRRRPPRE VERPSATIRY EPVAEPEPWC SFCSWDLILI LLVMLGAGCF 

        70         80         90        100        110        120 
ILLHLYLYPN LEKVAPLPNI DPENAPYTWG TYRPHMYFGL RTRSPMSPLF GMMWYEQPNT 

       130        140        150        160        170        180 
IQRPHIRHWC NQDDRLPGYY WYEADGRHFG KQNISEAHKG VIQTDWINDA NGFAARVKLN 

       190        200        210        220        230        240 
MAPGRRYNVI LYLSAQEIGT RFRLGKHLSD VFHGYNELLG KFTMSLRLKD NTKLQTSHSV 

       250        260        270        280        290        300 
MLTDEKIPID RYHDFVVDNT QAYNAPNQPL NYILNEKHND EEGKFIAVQL NLGSQAEFDI 

       310        320        330        340        350        360 
ILQTEKLKGM KPEEFTNILR IRSYNFNKKY ENVFQLAGKN YTKTQLKMAK VSLSNMLGSV 

       370        380        390        400        410        420 
GYWYGHNRVL FNGIVQPYGP HVLFSAVPSR PFFPRGFLWD EGFHQMLIRK MDSKMTLEAI 

       430        440        450        460        470        480 
ASWMNAMDTS GWIPREMIVG SEAEAKVPAE FIPQKNDVAN PPTLFYVMDK LVNDEKTVGR 

       490        500        510        520        530        540 
YAGILKLLYP RLEKWFHWIR ITQSGPTRTT YRWRGRNETI KTELNPKTLS SGLDDFPRAS 

       550        560        570        580        590        600 
HPSDLEYHLD LRCWLALASR VLNRLAKSYG TDADYQRTAK AMEELNNFDS LVKDHWSEEA 

       610        620        630        640        650        660 
QGFFDYGKHS FDVALSPVPT PGSPRQFEYQ RVTSRAPSYT LVSDAFGYNN LFPMMLKLIP 

       670        680        690        700        710        720 
SKSPILKSML DKIRDPKILW TNYGLRSISR SSPYYMARNT EHDPPYWRGY IWINVNYMVL 

       730        740        750        760        770        780 
SSLRHYADQP GPYRENAENI FSELRANLVK NLATQFQKTG FLWENYDDRT GEGRGCHPFT 

       790 
GWSSLILLIM SDNLDT

« Hide

Isoform b [UniParc].

Checksum: 6A1A86F3F3752028
Show »

FASTA78991,697

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[2]"Identification of the hydrophobic glycoproteins of Caenorhabditis elegans."
Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J.
Glycobiology 15:952-964(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-153, MASS SPECTROMETRY.
[3]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-517, MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z68748 Genomic DNA. Translation: CAL36499.1.
Z68748 Genomic DNA. Translation: CAL36500.1.
PIRT20864.
RefSeqNP_001076681.1. NM_001083212.3.
NP_001076682.1. NM_001083213.3.

3D structure databases

ProteinModelPortalQ19426.
ModBaseSearch...

Protein family/group databases

CAZyGH63. Glycoside Hydrolase Family 63.

Proteomic databases

PaxDbQ19426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF13H10.4a.1; F13H10.4a.1; F13H10.4.
F13H10.4a.2; F13H10.4a.2; F13H10.4.
GeneID177998.
KEGGcel:CELE_F13H10.4.
UCSCF13H10.4a.1. c. elegans.

Organism-specific databases

CTD177998.
WormBaseF13H10.4a; CE40354; WBGene00008775.
F13H10.4b; CE40355; WBGene00008775.

Phylogenomic databases

eggNOGNOG305138.
GeneTreeENSGT00390000017452.
HOGENOMHOG000201473.
InParanoidQ19426.
KOK01228.
OMAGFAARVK.

Gene expression databases

ArrayExpressQ19426.

Family and domain databases

InterProIPR008928. 6-hairpin_glycosidase-like.
IPR004888. Glycoside_hydrolase_63.
[Graphical view]
PANTHERPTHR10412. PTHR10412. 1 hit.
PfamPF03200. Glyco_hydro_63. 1 hit.
[Graphical view]
SUPFAMSSF48208. Glyco_trans_6hp. 1 hit.
ProtoNetSearch...

Other

NextBio899290.

Entry information

Entry nameGCS1_CAEEL
AccessionPrimary (citable) accession number: Q19426
Secondary accession number(s): Q0G829, Q0G830
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents