Reviewed,
UniProtKB/Swiss-Prot Q19426 (GCS1_CAEEL)
Last modified
June 16, 2009.
Version 57.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Probable mannosyl-oligosaccharide glucosidase EC=3.2.1.106 Alternative name(s): Processing A-glucosidase I | ||
| Gene names |
| ||
| Organism | Caenorhabditis elegans [Complete proteome] | ||
| Taxonomic identifier | 6239 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 796 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically By similarity. |
| Catalytic activity | Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential. |
| Sequence similarities | Belongs to the glycosyl hydrolase 63 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Glycosidase Hydrolase |
| PTM | Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | locomotion Inferred from mutant phenotype. Source: WormBase oligosaccharide metabolic processInferred from electronic annotation. Source: InterPro positive regulation of growth rateInferred from mutant phenotype. Source: WormBase |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | mannosyl-oligosaccharide glucosidase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform a (identifier: Q19426-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform b (identifier: Q19426-2) The sequence of this isoform differs from the canonical sequence as follows: 1-7: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 796 | 796 | Probable mannosyl-oligosaccharide glucosidase | PRO_0000057715 | |||||
Regions | |||||||||
| Topological domain | 1 – 46 | 46 | Cytoplasmic Potential | ||||||
| Transmembrane | 47 – 67 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 68 – 796 | 729 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 153 | 1 | N-linked (GlcNAc...) Ref.2 | ||||||
| Glycosylation | 340 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 517 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 7 | 7 | Missing in isoform b. | VSP_021635 | |||||
Sequences
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References
| [1] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| [2] | "Identification of the hydrophobic glycoproteins of Caenorhabditis elegans." Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J. Glycobiology 15:952-964(2005) [PubMed: 15888633] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-153, MASS SPECTROMETRY. |
| [3] | "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-517, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| Z68748 Genomic DNA. Translation: CAL36499.1. Z68748 Genomic DNA. Translation: CAL36500.1. | |
| PIR | T20864. |
| RefSeq | NP_001076681.1. |
| UniGene | Cel.17548 |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH63. Glycoside Hydrolase Family 63. |
Genome annotation databases | |
| Ensembl | F13H10.4. Caenorhabditis elegans. [Contig view] |
| GeneID | 177998. |
| KEGG | cel:F13H10.4. |
Organism-specific databases | |
| WormBase | WBGene00008775. F13H10.4. |
| WormPep | F13H10.4a. CE40354. [WorfDB] F13H10.4b. CE40355. [WorfDB] |
Phylogenomic databases | |
| OMA | Q19426. IPREMIV. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.106. 672. |
Gene expression databases | |
| ArrayExpress | Q19426. |
Family and domain databases | |
| InterPro | IPR004888. Glyco_hydro_63. [Graphical view] |
| Pfam | PF03200. Glyco_hydro_63. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 899290. |
Entry information
| Entry name | GCS1_CAEEL | ||||||||
| Accession | Primary (citable) accession number: Q19426 Secondary accession number(s): Q0G829, Q0G830 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
