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Q19426

- GCS1_CAEEL

UniProt

Q19426 - GCS1_CAEEL

Protein

Probable mannosyl-oligosaccharide glucosidase

Gene

F13H10.4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically.By similarity

    Catalytic activityi

    Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.

    GO - Molecular functioni

    1. mannosyl-oligosaccharide glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. oligosaccharide metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_183106. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

    Protein family/group databases

    CAZyiGH63. Glycoside Hydrolase Family 63.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable mannosyl-oligosaccharide glucosidase (EC:3.2.1.106)
    Alternative name(s):
    Processing A-glucosidase I
    Gene namesi
    ORF Names:F13H10.4
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiF13H10.4a; CE40354; WBGene00008775.
    F13H10.4b; CE40355; WBGene00008775.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 796796Probable mannosyl-oligosaccharide glucosidasePRO_0000057715Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi153 – 1531N-linked (GlcNAc...)1 Publication
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi517 – 5171N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ19426.

    Structurei

    3D structure databases

    ProteinModelPortaliQ19426.
    SMRiQ19426. Positions 84-790.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4646CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini68 – 796729LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei47 – 6721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni70 – 13061Required for endoplasmic reticulum targetingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi2 – 87Endoplasmic reticulum targeting

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 63 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305138.
    GeneTreeiENSGT00390000017452.
    HOGENOMiHOG000201473.
    InParanoidiQ19426.
    KOiK01228.
    OMAiWKAPLYT.
    PhylomeDBiQ19426.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR004888. Glycoside_hydrolase_63.
    [Graphical view]
    PANTHERiPTHR10412:SF1. PTHR10412:SF1. 1 hit.
    PfamiPF03200. Glyco_hydro_63. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q19426-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHREHEEMHQ PSRRRRPPRE VERPSATIRY EPVAEPEPWC SFCSWDLILI    50
    LLVMLGAGCF ILLHLYLYPN LEKVAPLPNI DPENAPYTWG TYRPHMYFGL 100
    RTRSPMSPLF GMMWYEQPNT IQRPHIRHWC NQDDRLPGYY WYEADGRHFG 150
    KQNISEAHKG VIQTDWINDA NGFAARVKLN MAPGRRYNVI LYLSAQEIGT 200
    RFRLGKHLSD VFHGYNELLG KFTMSLRLKD NTKLQTSHSV MLTDEKIPID 250
    RYHDFVVDNT QAYNAPNQPL NYILNEKHND EEGKFIAVQL NLGSQAEFDI 300
    ILQTEKLKGM KPEEFTNILR IRSYNFNKKY ENVFQLAGKN YTKTQLKMAK 350
    VSLSNMLGSV GYWYGHNRVL FNGIVQPYGP HVLFSAVPSR PFFPRGFLWD 400
    EGFHQMLIRK MDSKMTLEAI ASWMNAMDTS GWIPREMIVG SEAEAKVPAE 450
    FIPQKNDVAN PPTLFYVMDK LVNDEKTVGR YAGILKLLYP RLEKWFHWIR 500
    ITQSGPTRTT YRWRGRNETI KTELNPKTLS SGLDDFPRAS HPSDLEYHLD 550
    LRCWLALASR VLNRLAKSYG TDADYQRTAK AMEELNNFDS LVKDHWSEEA 600
    QGFFDYGKHS FDVALSPVPT PGSPRQFEYQ RVTSRAPSYT LVSDAFGYNN 650
    LFPMMLKLIP SKSPILKSML DKIRDPKILW TNYGLRSISR SSPYYMARNT 700
    EHDPPYWRGY IWINVNYMVL SSLRHYADQP GPYRENAENI FSELRANLVK 750
    NLATQFQKTG FLWENYDDRT GEGRGCHPFT GWSSLILLIM SDNLDT 796

    Note: No experimental confirmation available.

    Length:796
    Mass (Da):92,646
    Last modified:November 28, 2006 - v2
    Checksum:iA9DB69788CBB1F09
    GO
    Isoform b (identifier: Q19426-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-7: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:789
    Mass (Da):91,697
    Checksum:i6A1A86F3F3752028
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 77Missing in isoform b. CuratedVSP_021635

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68748 Genomic DNA. Translation: CAL36499.1.
    Z68748 Genomic DNA. Translation: CAL36500.1.
    PIRiT20864.
    RefSeqiNP_001076681.1. NM_001083212.3. [Q19426-1]
    NP_001076682.1. NM_001083213.3. [Q19426-2]
    UniGeneiCel.17548.

    Genome annotation databases

    EnsemblMetazoaiF13H10.4a; F13H10.4a; WBGene00008775. [Q19426-1]
    GeneIDi177998.
    KEGGicel:CELE_F13H10.4.
    UCSCiF13H10.4a.1. c. elegans. [Q19426-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68748 Genomic DNA. Translation: CAL36499.1 .
    Z68748 Genomic DNA. Translation: CAL36500.1 .
    PIRi T20864.
    RefSeqi NP_001076681.1. NM_001083212.3. [Q19426-1 ]
    NP_001076682.1. NM_001083213.3. [Q19426-2 ]
    UniGenei Cel.17548.

    3D structure databases

    ProteinModelPortali Q19426.
    SMRi Q19426. Positions 84-790.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH63. Glycoside Hydrolase Family 63.

    Proteomic databases

    PaxDbi Q19426.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F13H10.4a ; F13H10.4a ; WBGene00008775 . [Q19426-1 ]
    GeneIDi 177998.
    KEGGi cel:CELE_F13H10.4.
    UCSCi F13H10.4a.1. c. elegans. [Q19426-1 ]

    Organism-specific databases

    CTDi 177998.
    WormBasei F13H10.4a ; CE40354 ; WBGene00008775 .
    F13H10.4b ; CE40355 ; WBGene00008775 .

    Phylogenomic databases

    eggNOGi NOG305138.
    GeneTreei ENSGT00390000017452.
    HOGENOMi HOG000201473.
    InParanoidi Q19426.
    KOi K01228.
    OMAi WKAPLYT.
    PhylomeDBi Q19426.

    Enzyme and pathway databases

    Reactomei REACT_183106. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

    Miscellaneous databases

    NextBioi 899290.
    PROi Q19426.

    Family and domain databases

    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR004888. Glycoside_hydrolase_63.
    [Graphical view ]
    PANTHERi PTHR10412:SF1. PTHR10412:SF1. 1 hit.
    Pfami PF03200. Glyco_hydro_63. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "Identification of the hydrophobic glycoproteins of Caenorhabditis elegans."
      Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J.
      Glycobiology 15:952-964(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-153, IDENTIFICATION BY MASS SPECTROMETRY.
    3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-517, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGCS1_CAEEL
    AccessioniPrimary (citable) accession number: Q19426
    Secondary accession number(s): Q0G829, Q0G830
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3