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Q19420 (IMPA1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inositol monophosphatase ttx-7
Short name=IMP
Short name=IMPase
EC=3.1.3.25
Alternative name(s):
Abnormal thermotaxis protein 7
Inositol-1(or 4)-monophosphatase
Gene names
Name:ttx-7
ORF Names:F13G3.5
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. Required in central interneurons of the mature nervous system for correct localization of synaptic components. Required for thermotaxis. Ref.1 Ref.3

Catalytic activity

Myo-inositol phosphate + H2O = myo-inositol + phosphate.

Cofactor

Magnesium By similarity.

Enzyme regulation

Inhibited by Li+. Ref.1

Pathway

Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Isoform 1 is strongly expressed in the AFD and RIA neurons of the head, in the tail and in coelomocytes. Isoform 1 is weakly expressed in nerve cord motor neurons, intestine, and the somatic gonad. Ref.1

Disruption phenotype

Worms lacking ttx-7 display defects in thermotaxis behavior and localization of synaptic proteins in RIA neurons yet appear morphologically normal. Ref.1 Ref.3

Sequence similarities

Belongs to the inositol monophosphatase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q19420-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q19420-2)

The sequence of this isoform differs from the canonical sequence as follows:
     159-198: LLSKGVLCQS...MRNQVMAGVR → FIAESVLILQ...KTVMFDKQCH

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Inositol monophosphatase ttx-7
PRO_0000142519

Regions

Region98 – 1014Substrate binding By similarity
Region204 – 2063Substrate binding By similarity

Sites

Metal binding761Magnesium 1 By similarity
Metal binding961Magnesium 1 By similarity
Metal binding961Magnesium 2 By similarity
Metal binding981Magnesium 1; via carbonyl oxygen By similarity
Metal binding991Magnesium 2 By similarity
Metal binding2301Magnesium 2 By similarity
Binding site761Substrate By similarity
Binding site2231Substrate By similarity
Binding site2301Substrate By similarity

Natural variations

Alternative sequence159 – 19840LLSKG…MAGVR → FIAESVLILQLGSIRSPVMQ KSFVDSYKTVMFDKQCH in isoform b.
VSP_029919

Experimental info

Mutagenesis2161G → E in nj40; induces defects in thermotaxis. Ref.1
Sequence conflict2061G → A in BAF62082. Ref.1
Sequence conflict2061G → A in BAF62083. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 8337733F9D60C7BC

FASTA28530,980
        10         20         30         40         50         60 
MVFQPIHEEE QVFVDYAIEL VKKAGTLVRT AFDSPESKVD TKSSNTDLVT ETDQAVEKLL 

        70         80         90        100        110        120 
IEGLSERFKG HRFIGEESVA GGAKIEWTDA PTWIIDPIDG TTNFVHRIPM IAICVGLAIK 

       130        140        150        160        170        180 
KQIRAGIVYN PITNELYLAQ LGKGAFKNGF PIRASKNQLL SKGVLCQSLG LHNRVQFGDR 

       190        200        210        220        230        240 
WLDIAQSNMR NQVMAGVRGH RSFGSGAINM VMVAQGSCDG YVEYGIHAWD VAAPSIIVTE 

       250        260        270        280 
AGGVVTDPTG SPFDVMSRKV LCAGTAELGR DLSACLTHVD FEPEA

« Hide

Isoform b [UniParc].

Checksum: 6F8F105B78DFBBCB
Show »

FASTA28230,715

References

« Hide 'large scale' references
[1]"Inositol monophosphatase regulates localization of synaptic components and behavior in the mature nervous system of C. elegans."
Tanizawa Y., Kuhara A., Inada H., Kodama E., Mizuno T., Mori I.
Genes Dev. 20:3296-3310(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-216.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Temperature sensing by an olfactory neuron in a circuit controlling behavior of C. elegans."
Kuhara A., Okumura M., Kimata T., Tanizawa Y., Takano R., Kimura K.D., Inada H., Matsumoto K., Mori I.
Science 320:803-807(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB303038 mRNA. Translation: BAF62082.1.
AB303039 mRNA. Translation: BAF62083.1.
Z71259 Genomic DNA. Translation: CAA95791.3.
Z71259 Genomic DNA. Translation: CAN86622.2.
PIRT20855.
RefSeqNP_001122453.2. NM_001128981.3.
NP_001122454.2. NM_001128982.3.

3D structure databases

ProteinModelPortalQ19420.
SMRQ19420. Positions 11-263.
ModBaseSearch...

Proteomic databases

PaxDbQ19420.
PRIDEQ19420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF13G3.5a; F13G3.5a; F13G3.5.
GeneID172477.
KEGGcel:CELE_F13G3.5.
UCSCF13G3.5a. c. elegans.

Organism-specific databases

CTD172477.
WormBaseF13G3.5a; CE41115; WBGene00008765; ttx-7.
F13G3.5b; CE41116; WBGene00008765; ttx-7.

Phylogenomic databases

eggNOGCOG0483.
GeneTreeENSGT00390000014699.
HOGENOMHOG000282238.
InParanoidQ19420.
KOK01092.
OMAAAINMVM.

Enzyme and pathway databases

UniPathwayUPA00823; UER00788.

Family and domain databases

InterProIPR020583. Inositol_monoP_metal-BS.
IPR020552. Inositol_monoPase_Li-sen.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERPTHR20854. PTHR20854. 1 hit.
PfamPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSPR00377. IMPHPHTASES.
PR00378. LIIMPHPHTASE.
PROSITEPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio875675.

Entry information

Entry nameIMPA1_CAEEL
AccessionPrimary (citable) accession number: Q19420
Secondary accession number(s): A5A8G3, A5A8G4, A5JYT5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 4, 2007
Last modified: May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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