Reviewed, UniProtKB/Swiss-Prot Q19311 (PUR4_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

ORF Names:

F10F2.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1343	1343	Probable phosphoribosylformylglycinamidine synthase		PRO_0000100402
Regions
	Domain	1072 – 1314	243	Glutamine amidotransferase type-1
	Nucleotide binding	314 – 325	12	ATP Potential
Sites
	Active site	1165	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q19311-1 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: EC1A26FD1D95198E	FASTA	1,343	148,629
	10 20 30 40 50 60 MTKFHVKLYA KAVESRKLDQ IQKDFEKKFN RKIDVSVEYC YHVITQEPEL ISSNWEKLVT 70 80 90 100 110 120 LLSHSPFETS VWKESQLHPE HGKNIEIGPR TAVKTAACTN ILSIFESSGI KNVERIERGI 130 140 150 160 170 180 RYLVEDDVDV NEFFEIAADK MTEAIYGNDV KFDDESHQIE KVFLIDVLES KQNLIKANEE 190 200 210 220 230 240 LGLALDQLDL DFYYDFFVNK VKKNPTDVEL FDLAQSDSEH SRHWFFRGEI WIDDRKRDGS 250 260 270 280 290 300 LMKTIRETLD SSNDNSLIAF CDNSSAIRGF ESVCRLRPND PTTVSPMIAI FPPSHLIYSA 310 320 330 340 350 360 ETHNFPTAVC PFQGATTGTG GRIRDIHATG RGAYEIAGTV GYSFGNLNLP GLPLPWEDET 370 380 390 400 410 420 FEYPTSISEP AKIAIEASNG ASDYGNKFGE PVISGFARSF GQRLENGERC EYLKPIMFSG 430 440 450 460 470 480 GIGAIDKDEV RKEPCAPHQK VVKIGGPVYR IGVGGGAASS VSVQGNRENQ LDFAAVQRGD 490 500 510 520 530 540 AEMGGKLHRV VRACAERIGG NPLMAIHDQG AGGNGNVIKE LVEGCGVTVK SDTFQLGDES 550 560 570 580 590 600 ISLRELWTAE YQENDAALVD ASLLDALQTI SKREKCHVSV VGEVEKEQRV KLLGKSGEIA 610 620 630 640 650 660 VDLDTRQLGE REKKVFKLKS APRVLKKLEL PENLTVRKAL KRVLMLPSVA SKRYLTCKVD 670 680 690 700 710 720 RSVTGLVAQQ QCVGPLHTPL ADVAVVALSH FDTVGGAVSL GEQPIKMLID AEKGARMCIA 730 740 750 760 770 780 ETIMNLIWAP ITDLKVHFLI YFYSKFQKLL LSLKDVKMSG NWMWAAKCDG EGARLVDAVG 790 800 810 820 830 840 ALCRGLREIG CAIDGGKDSL SMAVTAHGEV VKSPGTLVLS AYAPCTNVTK VVNPSLKAVP 850 860 870 880 890 900 GSKILWIKIG SSEEKMRLGG SALAQVYSQI GDDCPDIENF SEISKVFSIV QQLLNREELA 910 920 930 940 950 960 GPLRKPIILA GHDISDGGLL TAILEMAFAG NVSIDIDIKP PNQNIKPIDI LFAEECGILL 970 980 990 1000 1010 1020 EVSNPENVLH IFSEAGIKCQ EIGKASAVFG PDAHVKIHVN GHLEINEKLV DLREEWELVG 1030 1040 1050 1060 1070 1080 DRLGEFQTNP KSLKEAREVR RTCQKINYKC DFDWYYNPAF IHNEQYFSTA PRVAIIREEG 1090 1100 1110 1120 1130 1140 SNGDREMASA FTLAGFQTFD VTMTDILAGH TLEAYRGVAF VGGFSYADVL GSAKGWAAGV 1150 1160 1170 1180 1190 1200 QFNESVSKQF EAFRSRPDTF SYGVCNGCQL MAQLGWIGDE EQKGPTVFLD ENECGRFDSS 1210 1220 1230 1240 1250 1260 FGPVKIEKNV SIMLSGMENS VLGLWSSHGE GRFTYRNLQN FQNLKTNGQV CIRFCDDRGM 1270 1280 1290 1300 1310 1320 TGADHGSVKL PYPWNPNGSI DDVAAICSRD GRHLAMMPHA DRSFLTWQWA ESSEVPWNAR 1330 1340 FDQKTVALSP WIKMFRNAYN WCL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35598 Genomic DNA. Translation: CAA84656.1.
PIR	T20718.
RefSeq	NP_497942.1.
UniGene	Cel.5802
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q19311.
Genome annotation databases
Ensembl	F10F2.2; F10F2.2; F10F2.2; Caenorhabditis elegans. [Genome view]
GeneID	175608.
KEGG	cel:F10F2.2.
NMPDR	fig|6239.3.peg.9487.
UCSC	F10F2.2. c. elegans.
Organism-specific databases
CTD	175608.
WormBase	WBGene00008654. F10F2.2.
WormPep	F10F2.2. CE17651. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04477.
HOGENOM	HBG335309.
InParanoid	Q19311.
OMA	ERGIAYY.
PhylomeDB	Q19311.
Enzyme and pathway databases
BRENDA	6.3.5.3. 672.
Gene expression databases
ArrayExpress	Q19311.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	888888.

Entry information

Entry name

PUR4_CAEEL

Accession

Primary (citable) accession number: Q19311

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project