Q19311 (PUR4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

ORF Names:

F10F2.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1324	1324	Probable phosphoribosylformylglycinamidine synthase		PRO_0000100402
Regions
	Domain	1053 – 1295	243	Glutamine amidotransferase type-1
	Nucleotide binding	314 – 325	12	ATP Potential
Sites
	Active site	1146	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q19311 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: C6ECB88C5FAA3797	FASTA	1,324	146,259
	10 20 30 40 50 60 MTKFHVKLYA KAVESRKLDQ IQKDFEKKFN RKIDVSVEYC YHVITQEPEL ISSNWEKLVT 70 80 90 100 110 120 LLSHSPFETS VWKESQLHPE HGKNIEIGPR TAVKTAACTN ILSIFESSGI KNVERIERGI 130 140 150 160 170 180 RYLVEDDVDV NEFFEIAADK MTEAIYGNDV KFDDESHQIE KVFLIDVLES KQNLIKANEE 190 200 210 220 230 240 LGLALDQLDL DFYYDFFVNK VKKNPTDVEL FDLAQSDSEH SRHWFFRGEI WIDDRKRDGS 250 260 270 280 290 300 LMKTIRETLD SSNDNSLIAF CDNSSAIRGF ESVCRLRPND PTTVSPMIAI FPPSHLIYSA 310 320 330 340 350 360 ETHNFPTAVC PFQGATTGTG GRIRDIHATG RGAYEIAGTV GYSFGNLNLP GLPLPWEDET 370 380 390 400 410 420 FEYPTSISEP AKIAIEASNG ASDYGNKFGE PVISGFARSF GQRLENGERC EYLKPIMFSG 430 440 450 460 470 480 GIGAIDKDEV RKEPCAPHQK VVKIGGPVYR IGVGGGAASS VSVQGNRENQ LDFAAVQRGD 490 500 510 520 530 540 AEMGGKLHRV VRACAERIGG NPLMAIHDQG AGGNGNVIKE LVEGCGVTVK SDTFQLGDES 550 560 570 580 590 600 ISLRELWTAE YQENDAALVD ASLLDALQTI SKREKCHVSV VGEVEKEQRV KLLGKSGEIA 610 620 630 640 650 660 VDLDTRQLGE REKKVFKLKS APRVLKKLEL PENLTVRKAL KRVLMLPSVA SKRYLTCKVD 670 680 690 700 710 720 RSVTGLVAQQ QCVGPLHTPL ADVAVVALSH FDTVGGAVSL GEQPIKMLID AEKGARMCIA 730 740 750 760 770 780 ETIMNLIWAP ITDLKDVKMS GNWMWAAKCD GEGARLVDAV GALCRGLREI GCAIDGGKDS 790 800 810 820 830 840 LSMAVTAHGE VVKSPGTLVL SAYAPCTNVT KVVNPSLKAV PGSKILWIKI GSSEEKMRLG 850 860 870 880 890 900 GSALAQVYSQ IGDDCPDIEN FSEISKVFSI VQQLLNREEL AGPLRKPIIL AGHDISDGGL 910 920 930 940 950 960 LTAILEMAFA GNVSIDIDIK PPNQNIKPID ILFAEECGIL LEVSNPENVL HIFSEAGIKC 970 980 990 1000 1010 1020 QEIGKASAVF GPDAHVKIHV NGHLEINEKL VDLREEWELV GDRLGEFQTN PKSLKEAREV 1030 1040 1050 1060 1070 1080 RRTCQKINYK CDFDWYYNPA FIHNEQYFST APRVAIIREE GSNGDREMAS AFTLAGFQTF 1090 1100 1110 1120 1130 1140 DVTMTDILAG HTLEAYRGVA FVGGFSYADV LGSAKGWAAG VQFNESVSKQ FEAFRSRPDT 1150 1160 1170 1180 1190 1200 FSYGVCNGCQ LMAQLGWIGD EEQKGPTVFL DENECGRFDS SFGPVKIEKN VSIMLSGMEN 1210 1220 1230 1240 1250 1260 SVLGLWSSHG EGRFTYRNLQ NFQNLKTNGQ VCIRFCDDRG MTGADHGSVK LPYPWNPNGS 1270 1280 1290 1300 1310 1320 IDDVAAICSR DGRHLAMMPH ADRSFLTWQW AESSEVPWNA RFDQKTVALS PWIKMFRNAY NWCL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35598 Genomic DNA. Translation: CAA84656.2.
PIR	T20718.
UniGene	Cel.5802.
3D structure databases
ProteinModelPortal	Q19311.
SMR	Q19311. Positions 1051-1323.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3386428.
STRING	Q19311.
Proteomic databases
PRIDE	Q19311.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
NMPDR	fig|6239.3.peg.9487.
UCSC	F10F2.2. c. elegans.
Organism-specific databases
WormBase	F10F2.2; CE44312; WBGene00008654.
Phylogenomic databases
eggNOG	meNOG04477.
GeneTree	EMGT00050000017325.
HOGENOM	HBG335309.
InParanoid	Q19311.
OMA	VKAVGME.
PhylomeDB	Q19311.
Gene expression databases
ArrayExpress	Q19311.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	888888.

Entry information

Entry name

PUR4_CAEEL

Accession

Primary (citable) accession number: Q19311

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project