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Protein

Probable phosphoribosylformylglycinamidine synthase

Gene

F10F2.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).By similarity

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable phosphoribosylformylglycinamidine synthase (F10F2.2)
  2. no protein annotated in this organism
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei681 – 6811ATP; via carbonyl oxygenBy similarity
Metal bindingi682 – 6821MagnesiumBy similarity
Metal bindingi721 – 7211MagnesiumBy similarity
Metal bindingi725 – 7251MagnesiumBy similarity
Metal bindingi894 – 8941MagnesiumBy similarity
Binding sitei896 – 8961ATPBy similarity
Active sitei1146 – 11461NucleophileBy similarity
Active sitei1280 – 12801By similarity
Active sitei1282 – 12821By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi314 – 32512ATPSequence analysisAdd
BLAST
Nucleotide bindingi394 – 3963ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoribosylformylglycinamidine synthase (EC:6.3.5.3)
Short name:
FGAM synthase
Short name:
FGAMS
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Short name:
FGAR amidotransferase
Short name:
FGAR-AT
Formylglycinamide ribotide amidotransferase
Gene namesi
ORF Names:F10F2.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF10F2.2; CE44312; WBGene00008654.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13241324Probable phosphoribosylformylglycinamidine synthasePRO_0000100402Add
BLAST

Proteomic databases

EPDiQ19311.
PaxDbiQ19311.
PRIDEiQ19311.

PTM databases

iPTMnetiQ19311.

Interactioni

Protein-protein interaction databases

IntActiQ19311. 1 interaction.
MINTiMINT-3386428.
STRINGi6239.F10F2.2.

Structurei

3D structure databases

ProteinModelPortaliQ19311.
SMRiQ19311. Positions 298-587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1053 – 1295243Glutamine amidotransferase type-1Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1907. Eukaryota.
COG0046. LUCA.
COG0047. LUCA.
GeneTreeiENSGT00390000007600.
HOGENOMiHOG000261358.
InParanoidiQ19311.
KOiK01952.
OMAiLSANWMW.
OrthoDBiEOG7353X4.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 2 hits.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q19311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKFHVKLYA KAVESRKLDQ IQKDFEKKFN RKIDVSVEYC YHVITQEPEL
60 70 80 90 100
ISSNWEKLVT LLSHSPFETS VWKESQLHPE HGKNIEIGPR TAVKTAACTN
110 120 130 140 150
ILSIFESSGI KNVERIERGI RYLVEDDVDV NEFFEIAADK MTEAIYGNDV
160 170 180 190 200
KFDDESHQIE KVFLIDVLES KQNLIKANEE LGLALDQLDL DFYYDFFVNK
210 220 230 240 250
VKKNPTDVEL FDLAQSDSEH SRHWFFRGEI WIDDRKRDGS LMKTIRETLD
260 270 280 290 300
SSNDNSLIAF CDNSSAIRGF ESVCRLRPND PTTVSPMIAI FPPSHLIYSA
310 320 330 340 350
ETHNFPTAVC PFQGATTGTG GRIRDIHATG RGAYEIAGTV GYSFGNLNLP
360 370 380 390 400
GLPLPWEDET FEYPTSISEP AKIAIEASNG ASDYGNKFGE PVISGFARSF
410 420 430 440 450
GQRLENGERC EYLKPIMFSG GIGAIDKDEV RKEPCAPHQK VVKIGGPVYR
460 470 480 490 500
IGVGGGAASS VSVQGNRENQ LDFAAVQRGD AEMGGKLHRV VRACAERIGG
510 520 530 540 550
NPLMAIHDQG AGGNGNVIKE LVEGCGVTVK SDTFQLGDES ISLRELWTAE
560 570 580 590 600
YQENDAALVD ASLLDALQTI SKREKCHVSV VGEVEKEQRV KLLGKSGEIA
610 620 630 640 650
VDLDTRQLGE REKKVFKLKS APRVLKKLEL PENLTVRKAL KRVLMLPSVA
660 670 680 690 700
SKRYLTCKVD RSVTGLVAQQ QCVGPLHTPL ADVAVVALSH FDTVGGAVSL
710 720 730 740 750
GEQPIKMLID AEKGARMCIA ETIMNLIWAP ITDLKDVKMS GNWMWAAKCD
760 770 780 790 800
GEGARLVDAV GALCRGLREI GCAIDGGKDS LSMAVTAHGE VVKSPGTLVL
810 820 830 840 850
SAYAPCTNVT KVVNPSLKAV PGSKILWIKI GSSEEKMRLG GSALAQVYSQ
860 870 880 890 900
IGDDCPDIEN FSEISKVFSI VQQLLNREEL AGPLRKPIIL AGHDISDGGL
910 920 930 940 950
LTAILEMAFA GNVSIDIDIK PPNQNIKPID ILFAEECGIL LEVSNPENVL
960 970 980 990 1000
HIFSEAGIKC QEIGKASAVF GPDAHVKIHV NGHLEINEKL VDLREEWELV
1010 1020 1030 1040 1050
GDRLGEFQTN PKSLKEAREV RRTCQKINYK CDFDWYYNPA FIHNEQYFST
1060 1070 1080 1090 1100
APRVAIIREE GSNGDREMAS AFTLAGFQTF DVTMTDILAG HTLEAYRGVA
1110 1120 1130 1140 1150
FVGGFSYADV LGSAKGWAAG VQFNESVSKQ FEAFRSRPDT FSYGVCNGCQ
1160 1170 1180 1190 1200
LMAQLGWIGD EEQKGPTVFL DENECGRFDS SFGPVKIEKN VSIMLSGMEN
1210 1220 1230 1240 1250
SVLGLWSSHG EGRFTYRNLQ NFQNLKTNGQ VCIRFCDDRG MTGADHGSVK
1260 1270 1280 1290 1300
LPYPWNPNGS IDDVAAICSR DGRHLAMMPH ADRSFLTWQW AESSEVPWNA
1310 1320
RFDQKTVALS PWIKMFRNAY NWCL
Length:1,324
Mass (Da):146,259
Last modified:July 27, 2011 - v3
Checksum:iC6ECB88C5FAA3797
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35598 Genomic DNA. Translation: CAA84656.2.
PIRiT20718.
RefSeqiNP_497942.2. NM_065541.5.
UniGeneiCel.5802.

Genome annotation databases

EnsemblMetazoaiF10F2.2; F10F2.2; WBGene00008654.
GeneIDi175608.
KEGGicel:CELE_F10F2.2.
UCSCiF10F2.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35598 Genomic DNA. Translation: CAA84656.2.
PIRiT20718.
RefSeqiNP_497942.2. NM_065541.5.
UniGeneiCel.5802.

3D structure databases

ProteinModelPortaliQ19311.
SMRiQ19311. Positions 298-587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ19311. 1 interaction.
MINTiMINT-3386428.
STRINGi6239.F10F2.2.

PTM databases

iPTMnetiQ19311.

Proteomic databases

EPDiQ19311.
PaxDbiQ19311.
PRIDEiQ19311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF10F2.2; F10F2.2; WBGene00008654.
GeneIDi175608.
KEGGicel:CELE_F10F2.2.
UCSCiF10F2.2. c. elegans.

Organism-specific databases

CTDi175608.
WormBaseiF10F2.2; CE44312; WBGene00008654.

Phylogenomic databases

eggNOGiKOG1907. Eukaryota.
COG0046. LUCA.
COG0047. LUCA.
GeneTreeiENSGT00390000007600.
HOGENOMiHOG000261358.
InParanoidiQ19311.
KOiK01952.
OMAiLSANWMW.
OrthoDBiEOG7353X4.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
ReactomeiR-CEL-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ19311.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 2 hits.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiPUR4_CAEEL
AccessioniPrimary (citable) accession number: Q19311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.