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Protein

Zinc metalloproteinase nas-14

Gene

nas-14

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable metalloprotease.By similarity

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi210Zinc; catalyticPROSITE-ProRule annotation1
Active sitei211PROSITE-ProRule annotation1
Metal bindingi214Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi220Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A23.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-14 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 14
Gene namesi
Name:nas-14
ORF Names:F09E8.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF09E8.6; CE35854; WBGene00003533; nas-14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002891826 – 503Zinc metalloproteinase nas-14Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi192N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi380 ↔ 414PROSITE-ProRule annotation
Disulfide bondi387 ↔ 407PROSITE-ProRule annotation
Disulfide bondi396 ↔ 411PROSITE-ProRule annotation
Glycosylationi437N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi469 ↔ 503PROSITE-ProRule annotation
Disulfide bondi476 ↔ 496PROSITE-ProRule annotation
Disulfide bondi485 ↔ 500PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ19269.
PeptideAtlasiQ19269.

Expressioni

Gene expression databases

BgeeiWBGene00003533.

Interactioni

Protein-protein interaction databases

STRINGi6239.F09E8.6.

Structurei

3D structure databases

ProteinModelPortaliQ19269.
SMRiQ19269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini380 – 414ShKT 1PROSITE-ProRule annotationAdd BLAST35
Domaini469 – 503ShKT 2PROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi320 – 333Poly-ThrAdd BLAST14
Compositional biasi337 – 340Poly-Thr4
Compositional biasi354 – 358Poly-Thr5
Compositional biasi362 – 368Poly-Thr7
Compositional biasi419 – 425Poly-Thr7
Compositional biasi457 – 462Poly-Thr6

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 2 ShKT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000016384.
InParanoidiQ19269.
KOiK08076.
OMAiKGIIAHE.
OrthoDBiEOG091G009U.
PhylomeDBiQ19269.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 2 hits.
[Graphical view]
PRINTSiPR00480. ASTACIN.
SMARTiSM00254. ShKT. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q19269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLYSLFHC SAFLVGFTLS VGVLPIPNEH AASIKAKFDD YAEHYLLPED
60 70 80 90 100
FHNAETAPVK KPTDAEIESM QNSLLFEGDI MGVPEIEKSD ILKRLRDDPL
110 120 130 140 150
LDEDEIFRKP FHSALNLVTY PDKLWPEGQV PYMLEEGMTN DQRTAIAQAF
160 170 180 190 200
DEYKTKTCVR FVPKTDDDFD YIYVKRNVAF GCSSYVGRAG GNQTVSLEVD
210 220 230 240 250
KCFSKGIIAH ELMHALGFFH EHSRTDRDDF VDINEDNIRP GMMRNFEKYP
260 270 280 290 300
RKIIDSLGMP YDYESVMHYH KLAFSRNGKP TIIPKDNEAD VGQRYKLSEM
310 320 330 340 350
DSKKVNKLYQ CGEYSKTSST TTTTTTTTTT TTTEEPTTTT EVEEKPKDKK
360 370 380 390 400
VSSTTTTTKK PTTTTTTTPK PVERSRNKKC EDLNAHCGMW EQLGHCQHSV
410 420 430 440 450
KYMAHYCRKA CNLCEVEVTT TTTTTPKPVP RNKEKENKSA SSTTRGTSTA
460 470 480 490 500
TSTTPKTTTT TTSAPKEKCE DKNLFCSYWA KIGECNSESK FMKIFCKASC

GKC
Length:503
Mass (Da):56,911
Last modified:July 5, 2004 - v2
Checksum:i1E28A52ECC044B27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73896 Genomic DNA. Translation: CAA98057.2.
AJ561207 mRNA. Translation: CAD99209.1.
PIRiT20658.
RefSeqiNP_502533.2. NM_070132.2.
UniGeneiCel.13155.

Genome annotation databases

EnsemblMetazoaiF09E8.6; F09E8.6; WBGene00003533.
GeneIDi184247.
KEGGicel:CELE_F09E8.6.
UCSCiF09E8.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73896 Genomic DNA. Translation: CAA98057.2.
AJ561207 mRNA. Translation: CAD99209.1.
PIRiT20658.
RefSeqiNP_502533.2. NM_070132.2.
UniGeneiCel.13155.

3D structure databases

ProteinModelPortaliQ19269.
SMRiQ19269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F09E8.6.

Protein family/group databases

MEROPSiM12.A23.

Proteomic databases

PaxDbiQ19269.
PeptideAtlasiQ19269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF09E8.6; F09E8.6; WBGene00003533.
GeneIDi184247.
KEGGicel:CELE_F09E8.6.
UCSCiF09E8.6. c. elegans.

Organism-specific databases

CTDi184247.
WormBaseiF09E8.6; CE35854; WBGene00003533; nas-14.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000016384.
InParanoidiQ19269.
KOiK08076.
OMAiKGIIAHE.
OrthoDBiEOG091G009U.
PhylomeDBiQ19269.

Miscellaneous databases

PROiQ19269.

Gene expression databases

BgeeiWBGene00003533.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 2 hits.
[Graphical view]
PRINTSiPR00480. ASTACIN.
SMARTiSM00254. ShKT. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAS14_CAEEL
AccessioniPrimary (citable) accession number: Q19269
Secondary accession number(s): Q7Z0N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.