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Protein

Protein kinase C-like 3

Gene

pkc-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the normal progression of embryogenesis and viability of the organism (PubMed:9422779). Plays an indispensable role in establishing embryonic polarity and in recruiting and maintaining par-6 to the periphery, through interaction with par-3 (PubMed:9716526, PubMed:9834192). Required for epithelial cell polarity in the distal spermatheca (PubMed:15151982). Phosphorylates serine residues of num-1 (PubMed:11134025). Required for the expression of antimicrobial peptide nlp-29 in response in response to fungal infection or physical injury (PubMed:19380113).6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei282ATPPROSITE-ProRule annotationBy similarity1
Active sitei377Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri127 – 177Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi259 – 267ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein domain specific binding Source: UniProtKB
  • protein kinase activity Source: WormBase
  • protein kinase C activity Source: UniProtKB-EC
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • defense response to fungus Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • establishment or maintenance of cell polarity Source: UniProtKB
  • gastrulation Source: UniProtKB-KW
  • gonadal mesoderm development Source: UniProtKB-KW
  • gonad development Source: UniProtKB
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: WormBase
  • protein localization Source: WormBase
  • response to wounding Source: WormBase
  • single fertilization Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Fertilization, Gastrulation, Gonadal differentiation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-1912408 Pre-NOTCH Transcription and Translation
R-CEL-5218921 VEGFR2 mediated cell proliferation
SignaLinkiQ19266

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C-like 3 (EC:2.7.11.13)
Alternative name(s):
Atypical protein kinase C-3
Short name:
aPKC3
Gene namesi
Name:pkc-3Imported
Synonyms:aPKC
ORF Names:F09E5.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi

Organism-specific databases

WormBaseiF09E5.1 ; CE02604 ; WBGene00004034 ; pkc-3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Worms show defects in embryogenesis. Mutant embryos show defects in their polarity and cleavage patterns which disrupt hatching (PubMed:9716526). RNAi-mediated knockdown causes a complete loss of nlp-29 expression following fungal infection by D.coniospora or physical injury (PubMed:19380113).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi214I → A: Prevents binding with num-1 (isoform a and isoform c). 1 Publication1
Mutagenesisi216N → A: Prevents binding with num-1 (isoform a and isoform c). 1 Publication1
Mutagenesisi219F → A: Prevents binding with num-1 (isoform a and isoform c). 1 Publication1
Mutagenesisi222H → A: No effect on binding with num-1 (isoform a and isoform c). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557371 – 597Protein kinase C-like 3Add BLAST597

Proteomic databases

EPDiQ19266
PaxDbiQ19266
PeptideAtlasiQ19266
PRIDEiQ19266

PTM databases

iPTMnetiQ19266

Expressioni

Tissue specificityi

Abundantly expressed in the anterior and posterior pharyngeal bulbs, the pharyngeal-intestinal valve, vulval cells, and a region at the posterior end of the intestine that corresponds to site of co-assembly of the intestinal-rectal valve and anal sphincter, and depressor muscles. Modest expression apparent in four hypodermal cells that provide cuticle and muscle anchorage in the tail. Strong expression observed in discrete patches and spots in vulval cells and in somatic cells of the spermatheca. Transiently coexpressed and colocalized with par-6 and par-3, asymmetrically in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae.3 Publications

Developmental stagei

Expressed throughout development, at highest level in the L1 stage and lowest in L3. Peripheral staining pattern in early embryos. The staining in 1-cell embryos is weak and uniform just after the completion of meiosis, but increases in intensity and becomes concentrated at the anterior periphery during pronuclear migration. The peripheral staining becomes restricted to about 50% embryo length during the pronuclear meeting and pronuclear fusion stage. By early anaphase, the staining extends posteriorly beyond the midline of the zygote and covers about 60% of the total length of embryos. In 2- and 4-cell stages, staining is uniform at the periphery of the AB cell, its daughters and the EMS cell, but peripheral staining in P1 and P2 is restricted to the boundaries with other blastomeres.2 Publications

Gene expression databases

BgeeiWBGene00004034

Interactioni

Subunit structurei

Interaction with par-3 required for the peripheral localization of par-6 and to form a par-3/par-6/pkc-3 complex, which is activated when cdc-42 interacts with par-6. Binds avidly to the phosphotyrosine interaction domain (PID) of a novel pkc-3 adapter protein num-1, which enables tethering and targeting of pkc-3 to the cell periphery.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi39258, 3 interactors
DIPiDIP-24814N
IntActiQ19266, 3 interactors
MINTiQ19266
STRINGi6239.F09E5.1.2

Structurei

3D structure databases

ProteinModelPortaliQ19266
SMRiQ19266
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 95PB1PROSITE-ProRule annotationAdd BLAST84
Domaini253 – 522Protein kinasePROSITE-ProRule annotationAdd BLAST270
Domaini524 – 595AGC-kinase C-terminalAdd BLAST72

Domaini

Residues 212-224 are capable of binding the PID domain of num-1 isoform a and isoform c.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri127 – 177Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0695 Eukaryota
ENOG410ZMG2 LUCA
GeneTreeiENSGT00820000126964
HOGENOMiHOG000233033
InParanoidiQ19266
KOiK06069
OMAiEPMMPMD
OrthoDBiEOG091G03Q9
PhylomeDBiQ19266

Family and domain databases

CDDicd00029 C1, 1 hit
cd06404 PB1_aPKC, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR034877 PB1_aPKC
IPR000270 PB1_dom
IPR002219 PE/DAG-bd
IPR012233 PKC
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00564 PB1, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
PIRSFiPIRSF000554 PKC_zeta, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00666 PB1, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS51745 PB1, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q19266-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPTSLEED GDIKLKTRFQ GQVVVLYARP PLILDDFFAL LKDACKQHKK
60 70 80 90 100
QDITVKWIDE DGDPISIDSQ MELDEAVRCL NSSQEAELNI HVFVGKPELP
110 120 130 140 150
GLPCQGEDKT VYRRGARRWK KIYLYNGHRF QAKRLNRRIQ CFICHDYIWG
160 170 180 190 200
IGRQGFRCVD CRLCVHKKCH RHVRTHCGQA LQGPNIIPMA PASGSLKGAR
210 220 230 240 250
SNTSSSTTRS GGGIDNGAFH EHEIESPGST SHDASRAMNG NGSSKWAVSL
260 270 280 290 300
NDFRLLTVIG RGSYAKVVQA EHVSTRQIYA IKIIKKEMFN EDEDIDWVQT
310 320 330 340 350
EKSVFEAASN YPFLVGLHSC FQTESRLFFV IEFVPGGDLM FHMQQQRKLP
360 370 380 390 400
EEHARFYSGE IILALHFLHS RGIIYRDLKL DNVLIDAEGH IKLTDYGMCK
410 420 430 440 450
ENIKDGDLTS TFCGTPNYIA PEILRGDEYG FSVDWWALGV LMFEMMAGRS
460 470 480 490 500
PFDIVGMQNS EENTEDYLFQ IILERQIRIP RSLSVRASGI LKGFLNKDPT
510 520 530 540 550
ERLGCKLDIN EGLRDMKEHQ FFRGFIDWEA LEQKAVAPPY HPAVESDRDL
560 570 580 590
THFDHQFTDE LPQLSPDNPD VIARIDQSEF DGFEYVNPLQ MSREDSV
Length:597
Mass (Da):68,035
Last modified:November 1, 1996 - v1
Checksum:iA92760E880DB11B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007320 mRNA Translation: BAF80143.1
AF025666 mRNA Translation: AAB88885.1
FO081044 Genomic DNA Translation: CCD68754.1
PIRiT16006
RefSeqiNP_495011.1, NM_062610.6
UniGeneiCel.19483

Genome annotation databases

EnsemblMetazoaiF09E5.1; F09E5.1; WBGene00004034
GeneIDi173914
KEGGicel:CELE_F09E5.1
UCSCiF09E5.1.2 c. elegans

Entry informationi

Entry nameiKPC3_CAEEL
AccessioniPrimary (citable) accession number: Q19266
Secondary accession number(s): A7VJC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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