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Protein

Von Hippel-Lindau tumor suppressor homolog

Gene

vhl-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the response to variation in environmental oxygen levels by targeting the hypoxia-inducible transcription factor hif-1 for proteasomal degradation when oxygen levels are normal (around 20%) (PubMed:11595184). By regulating hif-1 expression, plays a role in iron homeostasis, aging, heat acclimation and progeny size (PubMed:12686697, PubMed:19372390, PubMed:22396654). Mediates resistance to enteropathogenic E.coli (PubMed:16091039). Mediates susceptibility to B.thuringiensis pore-forming toxins (PubMed:20011506). Not involved in P.aeruginosa susceptibility (PubMed:20865124).7 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein ubiquitination Source: GO_Central
  • response to hypoxia Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Von Hippel-Lindau tumor suppressor homologImported
Gene namesi
Name:vhl-1Imported
ORF Names:F08G12.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF08G12.4; CE03169; WBGene00006922; vhl-1.

Subcellular locationi

GO - Cellular componenti

  • Cul2-RING ubiquitin ligase complex Source: WormBase
  • nucleus Source: GO_Central
  • VCB complex Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Increased mortality induced by B.thuringiensis pore-forming toxins Cry21A and Cry21B (PubMed:20011506). Increased mortality and sensitivity to paralysis induced by enteropathogenic E.coli infection (PubMed:16091039). Increased mortality upon heat stress (PubMed:12686697). RNAi-mediated knockdown causes an enhanced resistance to polyglutamine or beta-amyloid-mediated paralysis and an increase in adult life span.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174Von Hippel-Lindau tumor suppressor homologCuratedPRO_0000433368Add
BLAST

Proteomic databases

PaxDbiQ19213.

Interactioni

Subunit structurei

Interacts with hif-1 (hydroxylated on 'Pro-621'); the interaction induces hif-1 degradation (PubMed:11595184). May be a component of the cullin E3 ubiquitin ligase complex (By similarity).By similarity1 Publication

Protein-protein interaction databases

IntActiQ19213. 5 interactions.
STRINGi6239.F08G12.4.

Structurei

3D structure databases

ProteinModelPortaliQ19213.
SMRiQ19213. Positions 16-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VHL family.Curated

Phylogenomic databases

eggNOGiKOG4710. Eukaryota.
ENOG4111PRU. LUCA.
HOGENOMiHOG000021082.
InParanoidiQ19213.
KOiK03871.
OMAiQPTKYGT.
OrthoDBiEOG7VMP7B.
PhylomeDBiQ19213.

Family and domain databases

Gene3Di2.60.40.780. 1 hit.
InterProiIPR024053. VHL_beta_dom.
IPR022772. VHL_tumour_suppress_b/a_dom.
[Graphical view]
PfamiPF01847. VHL. 1 hit.
[Graphical view]
SUPFAMiSSF49468. SSF49468. 1 hit.

Sequencei

Sequence statusi: Complete.

Q19213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGSMDDDG RLFPDLGSST HDNREIRVRF LNRCAYPVDV FWLNPSKQPT
60 70 80 90 100
KYGTLAQKKY LDIKTFKDHP WVARRSFDGC KVLVNEKEVF WPEPAPRMNL
110 120 130 140 150
IVRNHCVITM KVQSLREIAG RSFLRHNPTE VPNKIKGLPR ELQFEVKHFL
160 170
DRKQEYSEIV CRSIPPPGPQ RPQQ
Length:174
Mass (Da):20,345
Last modified:November 1, 1996 - v1
Checksum:iA082698EF932EAC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66561 Genomic DNA. Translation: CAA91456.1.
PIRiT20589.
RefSeqiNP_509889.1. NM_077488.3.
UniGeneiCel.11039.

Genome annotation databases

EnsemblMetazoaiF08G12.4; F08G12.4; WBGene00006922.
GeneIDi181321.
KEGGicel:CELE_F08G12.4.
UCSCiF08G12.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66561 Genomic DNA. Translation: CAA91456.1.
PIRiT20589.
RefSeqiNP_509889.1. NM_077488.3.
UniGeneiCel.11039.

3D structure databases

ProteinModelPortaliQ19213.
SMRiQ19213. Positions 16-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ19213. 5 interactions.
STRINGi6239.F08G12.4.

Proteomic databases

PaxDbiQ19213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF08G12.4; F08G12.4; WBGene00006922.
GeneIDi181321.
KEGGicel:CELE_F08G12.4.
UCSCiF08G12.4. c. elegans.

Organism-specific databases

CTDi181321.
WormBaseiF08G12.4; CE03169; WBGene00006922; vhl-1.

Phylogenomic databases

eggNOGiKOG4710. Eukaryota.
ENOG4111PRU. LUCA.
HOGENOMiHOG000021082.
InParanoidiQ19213.
KOiK03871.
OMAiQPTKYGT.
OrthoDBiEOG7VMP7B.
PhylomeDBiQ19213.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ19213.

Family and domain databases

Gene3Di2.60.40.780. 1 hit.
InterProiIPR024053. VHL_beta_dom.
IPR022772. VHL_tumour_suppress_b/a_dom.
[Graphical view]
PfamiPF01847. VHL. 1 hit.
[Graphical view]
SUPFAMiSSF49468. SSF49468. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. Cited for: FUNCTION, INTERACTION WITH HIF-1.
  3. "HIF-1 is required for heat acclimation in the nematode Caenorhabditis elegans."
    Treinin M., Shliar J., Jiang H., Powell-Coffman J.A., Bromberg Z., Horowitz M.
    Physiol. Genomics 14:17-24(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Paralysis and killing of Caenorhabditis elegans by enteropathogenic Escherichia coli requires the bacterial tryptophanase gene."
    Anyanful A., Dolan-Livengood J.M., Lewis T., Sheth S., Dezalia M.N., Sherman M.A., Kalman L.V., Benian G.M., Kalman D.
    Mol. Microbiol. 57:988-1007(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Hypoxia and the hypoxic response pathway protect against pore-forming toxins in C. elegans."
    Bellier A., Chen C.S., Kao C.Y., Cinar H.N., Aroian R.V.
    PLoS Pathog. 5:E1000689-E1000689(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Proteasomal regulation of the hypoxic response modulates aging in C. elegans."
    Mehta R., Steinkraus K.A., Sutphin G.L., Ramos F.J., Shamieh L.S., Huh A., Davis C., Chandler-Brown D., Kaeberlein M.
    Science 324:1196-1198(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "C. elegans SWAN-1 Binds to EGL-9 and regulates HIF-1-mediated resistance to the bacterial pathogen Pseudomonas aeruginosa PAO1."
    Shao Z., Zhang Y., Ye Q., Saldanha J.N., Powell-Coffman J.A.
    PLoS Pathog. 6:E1001075-E1001075(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Insulin/IGF-1 and hypoxia signaling act in concert to regulate iron homeostasis in Caenorhabditis elegans."
    Ackerman D., Gems D.
    PLoS Genet. 8:E1002498-E1002498(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVHL_CAEEL
AccessioniPrimary (citable) accession number: Q19213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.