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Protein

Niemann-Pick C1 protein homolog 1

Gene

ncr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake or utilization of cholesterol. Ncr-1 and ncr-2 act redundantly to prevent dauer larva formation under favorable growth conditions, and are required for the normal functioning of ADF, ASI and ASG neurons.1 Publication

GO - Biological processi

  • cholesterol homeostasis Source: WormBase
  • cholesterol transport Source: WormBase
  • dauer larval development Source: WormBase
  • positive regulation of growth rate Source: WormBase
  • regulation of oviposition Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

ReactomeiR-CEL-265473. Trafficking of dietary sterols.

Protein family/group databases

TCDBi2.A.6.6.8. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Niemann-Pick C1 protein homolog 1
Gene namesi
Name:ncr-1
Synonyms:npc-1
ORF Names:F02E8.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF02E8.6; CE29251; WBGene00003561; ncr-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei282 – 30221HelicalSequence analysisAdd
BLAST
Transmembranei353 – 37321HelicalSequence analysisAdd
BLAST
Transmembranei627 – 64721HelicalSequence analysisAdd
BLAST
Transmembranei665 – 68521HelicalSequence analysisAdd
BLAST
Transmembranei697 – 71721HelicalSequence analysisAdd
BLAST
Transmembranei746 – 76621HelicalSequence analysisAdd
BLAST
Transmembranei780 – 80021HelicalSequence analysisAdd
BLAST
Transmembranei856 – 87621HelicalSequence analysisAdd
BLAST
Transmembranei1126 – 114621HelicalSequence analysisAdd
BLAST
Transmembranei1157 – 117721HelicalSequence analysisAdd
BLAST
Transmembranei1179 – 119921HelicalSequence analysisAdd
BLAST
Transmembranei1226 – 124621HelicalSequence analysisAdd
BLAST
Transmembranei1260 – 128021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • late endosome Source: WormBase
  • lysosome Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Both ncr-1 single mutant and ncr-1 and ncr-2 double mutants exhibit slow embryonic and larval development, and hyperactive egg-laying behavior (egg-laying constitutive (egl-c) phenotype). Ncr-1 and ncr-2 double mutants inappropriately and transiently form dauer larvae under favorable conditions (dauer-constitutive (daf-c) phenotype).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 13831363Niemann-Pick C1 protein homolog 1PRO_0000023264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...)Sequence analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)1 Publication
Glycosylationi447 – 4471N-linked (GlcNAc...)Sequence analysis
Glycosylationi558 – 5581N-linked (GlcNAc...)Sequence analysis
Glycosylationi993 – 9931N-linked (GlcNAc...)Sequence analysis
Glycosylationi1082 – 10821N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ19127.
PRIDEiQ19127.

Interactioni

Protein-protein interaction databases

BioGridi45654. 1 interaction.
STRINGi6239.F02E8.6.

Structurei

3D structure databases

ProteinModelPortaliQ19127.
SMRiQ19127. Positions 57-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini627 – 800174SSDPROSITE-ProRule annotationCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the patched family.Curated
Contains 1 SSD (sterol-sensing) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1933. Eukaryota.
ENOG410XR54. LUCA.
GeneTreeiENSGT00800000124043.
HOGENOMiHOG000020287.
InParanoidiQ19127.
KOiK12385.
OMAiDQSTFIN.
OrthoDBiEOG7QRQT0.
PhylomeDBiQ19127.

Family and domain databases

InterProiIPR032190. NPC1_N.
IPR003392. Ptc/Disp.
IPR000731. SSD.
[Graphical view]
PfamiPF16414. NPC1_N. 1 hit.
PF02460. Patched. 2 hits.
[Graphical view]
PROSITEiPS50156. SSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q19127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLLIFCLL FGSIFHHGDA GCIMRGLCQK HTENAYGPCV TNDTNVEPTA
60 70 80 90 100
FDKTHPAYEK MVEFCPHLLT GDNKLCCTPS QAEGLTKQIA QARHILGRCP
110 120 130 140 150
SCFDNFAKLW CEFTCSPNQQ DFVSISEMKP IEKKEGFTPE YQPAEAYVNT
160 170 180 190 200
VEYRLSTDFA EGMFSSCKDV TFGGQPALRV MCTSTPCTLT NWLEFIGTQN
210 220 230 240 250
LDLNIPIHTK FLLYDPIKTP PSDRSTYMNV NFTGCDKSAR VGWPACSTSE
260 270 280 290 300
CNKEEYANLI DLDDGKTSGQ TCNVHGIACL NIFVMLAFIG SLAVLLCVGF
310 320 330 340 350
VFTSYDEDYT NLRQTQSGEE SPKRNRIKRT GAWIHNFMEN NARDIGMMAG
360 370 380 390 400
RNPKSHFFIG CAVLIFCLPG MIYHKESTNV VDMWSSPRSR ARQEEMVFNA
410 420 430 440 450
NFGRPQRYQQ IMLLSHRDFQ SSGKLYGPVF HKDIFEELFD ILNAIKNIST
460 470 480 490 500
QDSDGRTITL DDVCYRPMGP GYDCLIMSPT NYFQGNKEHL DMKSNKEETV
510 520 530 540 550
SEDDDAFDYF SSEATTDEWM NHMAACIDQP MSQKTKSGLS CMGTYGGPSA
560 570 580 590 600
PNMVFGKNST NHQAANSIMM TILVTQRTEP EIQKAELWEK EFLKFCKEYR
610 620 630 640 650
EKSPKVIFSF MAERSITDEI ENDAKDEIVT VVIALAFLIG YVTFSLGRYF
660 670 680 690 700
VCENQLWSIL VHSRICLGML SVIINLLSSF CSWGIFSMFG IHPVKNALVV
710 720 730 740 750
QFFVVTLLGV CRTFMVVKYY AQQRVSMPYM SPDQCPEIVG MVMAGTMPAM
760 770 780 790 800
FSSSLGCAFS FFIGGFTDLP AIRTFCLYAG LAVLIDVVLH CTIFLALFVW
810 820 830 840 850
DTQRELNGKP EFFFPYQIKD LLGAYLIGRQ RATDTFMTQF FHFQVAPFLM
860 870 880 890 900
HRMTRIITGI IFIASFITTV ILSSKISVGF DQSMAFTEKS YISTHFRYLD
910 920 930 940 950
KFFDVGPPVF FTVDGELDWH RPDVQNKFCT FPGCSDTSFG NIMNYAVGHT
960 970 980 990 1000
EQTYLSGEMY NWIDNYLEWI SRKSPCCKVY VHDPNTFCST NRNKSALDDK
1010 1020 1030 1040 1050
ACRTCMDFDY VANSYPKSSI MYHRPSIEVF YRHLRHFLED TPNSECVFGG
1060 1070 1080 1090 1100
RASFKDAISF TSRGRIQASQ FMTFHKKLSI SNSSDFIKAM DTARMVSRRL
1110 1120 1130 1140 1150
ERSIDDTAHV FAYSKIFPFY EQYSTIMPIL TTQLFITVVG VFGIICVTLG
1160 1170 1180 1190 1200
IDVKGAACAV ICQVSNYFHI VAFMYIFNIP VNALSATNLV MSSGILIEFS
1210 1220 1230 1240 1250
VNVLKGYACS LRQRAKDRAE STVGSIGPII LSGPVVTMAG STMFLSGAHL
1260 1270 1280 1290 1300
QIITVYFFKL FLITIVSSAV HALIILPILL AFGGSRGHGS SETSTNDNDE
1310 1320 1330 1340 1350
QHDACVLSPT AESHISNVEE GILNRPSLLD ASHILDPLLK AEGGIDKAID
1360 1370 1380
IITIDRSYPS TPSSLPCTSR MPRAHIEPDL RSL
Length:1,383
Mass (Da):155,457
Last modified:December 1, 2001 - v2
Checksum:iA1392ED28CF9D24C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081068 Genomic DNA. Translation: CCD83387.1.
PIRiT15961.
RefSeqiNP_508771.1. NM_076370.3.
UniGeneiCel.622.

Genome annotation databases

EnsemblMetazoaiF02E8.6; F02E8.6; WBGene00003561.
GeneIDi180719.
KEGGicel:CELE_F02E8.6.
UCSCiF02E8.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081068 Genomic DNA. Translation: CCD83387.1.
PIRiT15961.
RefSeqiNP_508771.1. NM_076370.3.
UniGeneiCel.622.

3D structure databases

ProteinModelPortaliQ19127.
SMRiQ19127. Positions 57-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45654. 1 interaction.
STRINGi6239.F02E8.6.

Protein family/group databases

TCDBi2.A.6.6.8. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiQ19127.
PRIDEiQ19127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF02E8.6; F02E8.6; WBGene00003561.
GeneIDi180719.
KEGGicel:CELE_F02E8.6.
UCSCiF02E8.6. c. elegans.

Organism-specific databases

CTDi180719.
WormBaseiF02E8.6; CE29251; WBGene00003561; ncr-1.

Phylogenomic databases

eggNOGiKOG1933. Eukaryota.
ENOG410XR54. LUCA.
GeneTreeiENSGT00800000124043.
HOGENOMiHOG000020287.
InParanoidiQ19127.
KOiK12385.
OMAiDQSTFIN.
OrthoDBiEOG7QRQT0.
PhylomeDBiQ19127.

Enzyme and pathway databases

ReactomeiR-CEL-265473. Trafficking of dietary sterols.

Miscellaneous databases

PROiQ19127.

Family and domain databases

InterProiIPR032190. NPC1_N.
IPR003392. Ptc/Disp.
IPR000731. SSD.
[Graphical view]
PfamiPF16414. NPC1_N. 1 hit.
PF02460. Patched. 2 hits.
[Graphical view]
PROSITEiPS50156. SSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "A model for Niemann-Pick type C disease in the nematode Caenorhabditis elegans."
    Sym M., Basson M., Johnson C.
    Curr. Biol. 10:527-530(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
  3. "Murine model of Niemann-Pick C disease: mutation in a cholesterol homeostasis gene."
    Loftus S.K., Morris J.A., Carstea E.D., Gu J.Z., Cummings C., Brown A., Ellison J., Ohno K., Rosenfeld M.A., Tagle D.A., Pentchev P.G., Pavan W.J.
    Science 277:232-235(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-231, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiNPC1_CAEEL
AccessioniPrimary (citable) accession number: Q19127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.