Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SLAM family member 5

Gene

Cd84

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:20962259). Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (PubMed:20962259). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells (By similarity). Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation (PubMed:20153220). In germinal centers involved in maintaining B cell tolerance and in preventing autoimmunity (PubMed:25801429). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (By similarity). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (PubMed:20628063).By similarityCurated5 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Cell adhesion, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
SLAM family member 5
Alternative name(s):
Leukocyte differentiation antigen CD84
Signaling lymphocytic activation molecule 5
CD_antigen: CD84
Gene namesi
Name:Cd84
Synonyms:Slamf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1336885. Cd84.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 221ExtracellularSequence analysisAdd BLAST200
Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
Topological domaini243 – 329CytoplasmicSequence analysisAdd BLAST87

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265Y → F: No effect on macrophage cytokine secretion. 1 Publication1
Mutagenesisi300Y → F: Modulates macrophage cytokine secretion. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000025203022 – 329SLAM family member 5Add BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi147N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 190PROSITE-ProRule annotation
Modified residuei280Phosphotyrosine; by LYNBy similarity1
Modified residuei300PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-280 and Tyr-300 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells.By similarity
N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ18PI6.
PaxDbiQ18PI6.
PeptideAtlasiQ18PI6.
PRIDEiQ18PI6.

PTM databases

iPTMnetiQ18PI6.
PhosphoSitePlusiQ18PI6.

Expressioni

Tissue specificityi

Predominantly expressed in hematopoietic tissues such as lymph node, spleen, thymus, and bone marrow. Detected also in lung.

Gene expression databases

BgeeiENSMUSG00000038147.
CleanExiMM_CD84.

Interactioni

Subunit structurei

Homodimer; via its extracellular domain. Forms a head to tail dimer with a CD48 molecule from another cell. Interacts with SH2 domain-containing proteins SH2D1A/SAP and SH2D1B/EAT-2. Interacts with tyrosine-protein phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 via its phosphorylated cytoplasmic domain, and this interaction is blocked by SH2D1A (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047024.

Structurei

3D structure databases

ProteinModelPortaliQ18PI6.
SMRiQ18PI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 129Ig-like V-typeAdd BLAST104
Domaini132 – 206Ig-like C2-typeAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi263 – 268ITSM 1By similarity6
Motifi298 – 303ITSM 2By similarity6

Domaini

The ITSMs (immunoreceptor tyrosine-based switch motifs) with the consensus sequence T-X-Y-X-X-[VI] present in SLAM family receptors have overlapping specificity for activating and inhibitory SH2 domain-containingbinding partners. Especially they mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. A 'two-out-of-three-pronged' mechanism is proposed involving threonine (position -2), phosphorylated tyrosine (position 0) and valine/isoleucine (position +3).By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVV0. Eukaryota.
ENOG410Z5M6. LUCA.
HOGENOMiHOG000013065.
HOVERGENiHBG093961.
InParanoidiQ18PI6.
KOiK06511.
TreeFamiTF334964.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q18PI6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQRHLWIWF LCLQTWSEAA GKDADPMVMN GILGESVTFL LNIQEPKKID
60 70 80 90 100
NIAWTSQSSV AFIKPGVNKA EVTITQGTYK GRIEIIDQKY DLVIRDLRME
110 120 130 140 150
DAGTYKADIN EENEETITKI YYLHIYRRLK TPKITQSLIS SLNNTCNITL
160 170 180 190 200
TCSVEKEEKD VTYSWSPFGE KSNVLQIVHS PMDQKLTYTC TAQNPVSNSS
210 220 230 240 250
DSVTVQQPCT DTPSFHPRHA VLPGGLAVLF LLILIPMLAF LFRLYKRRRD
260 270 280 290 300
RIVLEADDVS KKTVYAVVSR NAQPTESRIY DEIPQSKMLS CKEDPVTTIY
310 320
SSVQLSEKMK ETNMKDRSLP KALGNEIVV
Length:329
Mass (Da):37,378
Last modified:July 25, 2006 - v1
Checksum:iB3BF45FEA7B00FB6
GO
Isoform 2 (identifier: Q18PI6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-140: RLKTPKITQSLIS → KLWQHGALDLLLI
     141-329: Missing.

Show »
Length:140
Mass (Da):16,174
Checksum:iEB487F113EF3868D
GO
Isoform 3 (identifier: Q18PI6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: Missing.

Show »
Length:328
Mass (Da):37,307
Checksum:i95E1FA3C07E0FB77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27M → V in AAD02273 (PubMed:10079287).Curated1
Sequence conflicti27M → V in BAC29799 (PubMed:16141072).Curated1
Sequence conflicti27M → V in BAC30670 (PubMed:16141072).Curated1
Sequence conflicti27M → V in BAE32863 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti293E → K.3 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020857128 – 140RLKTP…QSLIS → KLWQHGALDLLLI in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_020858141 – 329Missing in isoform 2. 1 PublicationAdd BLAST189
Alternative sequenceiVSP_020859256Missing in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043445 mRNA. Translation: AAD02273.1.
AB196812 mRNA. Translation: BAE96315.1.
AB196813 mRNA. Translation: BAE96316.1.
AB196814 mRNA. Translation: BAE96317.1.
AB196815 mRNA. Translation: BAE96318.1.
AK037385 mRNA. Translation: BAC29799.1.
AK040694 mRNA. Translation: BAC30670.1.
AK154834 mRNA. Translation: BAE32863.1.
AK156464 mRNA. Translation: BAE33721.1.
AK157075 mRNA. Translation: BAE33953.1.
AK171067 mRNA. Translation: BAE42226.1.
CCDSiCCDS15503.1. [Q18PI6-1]
CCDS56657.1. [Q18PI6-2]
CCDS69979.1. [Q18PI6-3]
RefSeqiNP_001239401.1. NM_001252472.1.
NP_038517.1. NM_013489.3.
UniGeneiMm.259115.

Genome annotation databases

GeneIDi12523.
KEGGimmu:12523.
UCSCiuc007dpd.3. mouse. [Q18PI6-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043445 mRNA. Translation: AAD02273.1.
AB196812 mRNA. Translation: BAE96315.1.
AB196813 mRNA. Translation: BAE96316.1.
AB196814 mRNA. Translation: BAE96317.1.
AB196815 mRNA. Translation: BAE96318.1.
AK037385 mRNA. Translation: BAC29799.1.
AK040694 mRNA. Translation: BAC30670.1.
AK154834 mRNA. Translation: BAE32863.1.
AK156464 mRNA. Translation: BAE33721.1.
AK157075 mRNA. Translation: BAE33953.1.
AK171067 mRNA. Translation: BAE42226.1.
CCDSiCCDS15503.1. [Q18PI6-1]
CCDS56657.1. [Q18PI6-2]
CCDS69979.1. [Q18PI6-3]
RefSeqiNP_001239401.1. NM_001252472.1.
NP_038517.1. NM_013489.3.
UniGeneiMm.259115.

3D structure databases

ProteinModelPortaliQ18PI6.
SMRiQ18PI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047024.

PTM databases

iPTMnetiQ18PI6.
PhosphoSitePlusiQ18PI6.

Proteomic databases

MaxQBiQ18PI6.
PaxDbiQ18PI6.
PeptideAtlasiQ18PI6.
PRIDEiQ18PI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12523.
KEGGimmu:12523.
UCSCiuc007dpd.3. mouse. [Q18PI6-2]

Organism-specific databases

CTDi8832.
MGIiMGI:1336885. Cd84.

Phylogenomic databases

eggNOGiENOG410IVV0. Eukaryota.
ENOG410Z5M6. LUCA.
HOGENOMiHOG000013065.
HOVERGENiHBG093961.
InParanoidiQ18PI6.
KOiK06511.
TreeFamiTF334964.

Miscellaneous databases

PROiQ18PI6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038147.
CleanExiMM_CD84.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLAF5_MOUSE
AccessioniPrimary (citable) accession number: Q18PI6
Secondary accession number(s): Q18PI7
, Q3TBT1, Q3U0A2, Q8BFV0, Q9Z178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.