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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42NADUniRule annotation1
Binding sitei69NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi167Iron; shared with adjacent protomerUniRule annotation1
Binding sitei167NAD; shared with adjacent protomerUniRule annotation1
Metal bindingi182IronUniRule annotation1
Binding sitei257NAD; via amide nitrogenUniRule annotation1
Binding sitei267GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 62NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:HQ_1276A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloquadratum
Proteomesi
  • UP000001975 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002593791 – 307Thiamine thiazole synthaseAdd BLAST307

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ1276A

Structurei

3D structure databases

ProteinModelPortaliQ18KP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK03146
OMAiMTEFTDI
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Q18KP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFDEFADVS EAQVTRAIAK DWGNEFIDRV DTEVIIVGGG PSGLVTAKEL
60 70 80 90 100
AERGVDVTIV EKNNYLGGGF WLGGFLMNKV TVRDPAQSVL DELDVPYEES
110 120 130 140 150
DEASGLYVAD GPHAVSSLIK SACDAGAEVQ NMTEFTDVVT RENHRVGGIV
160 170 180 190 200
LNWTPVHALP RELTCVDPVA VESDLVVDST GHDAVVISKL SERGVLNAPG
210 220 230 240 250
IEHANEHNTG MDNTADDEYG APGHDSPGHD SMWVSRSEDQ VVEHTGVVHP
260 270 280 290 300
GVVATGMAVA TVEGLPRMGP TFGAMLLSGK RGAQACLNEL GREATPIDFT

PAAPADD
Length:307
Mass (Da):32,487
Last modified:July 25, 2006 - v1
Checksum:i5C160E7824F7330E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA Translation: CAJ51405.1
RefSeqiWP_011570564.1, NC_008212.1

Genome annotation databases

EnsemblBacteriaiCAJ51405; CAJ51405; HQ_1276A
GeneIDi4192223
KEGGihwa:HQ_1276A

Similar proteinsi

Entry informationi

Entry nameiTHI4_HALWD
AccessioniPrimary (citable) accession number: Q18KP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 25, 2006
Last modified: April 25, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health