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Q18KI0

- KAE1B_HALWD

UniProt

Q18KI0 - KAE1B_HALWD

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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

HQ_1341A

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi112 – 1121IronUniRule annotation
Metal bindingi116 – 1161IronUniRule annotation
Binding sitei165 – 1651Threonylcarbamoyl-AMPUniRule annotation
Binding sitei178 – 1781Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
Binding sitei182 – 1821Threonylcarbamoyl-AMPUniRule annotation
Binding sitei261 – 2611Threonylcarbamoyl-AMPUniRule annotation
Metal bindingi289 – 2891IronUniRule annotation
Binding sitei363 – 3631ATPUniRule annotation
Active sitei452 – 4521Proton acceptor; for kinase activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi347 – 3548ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHWAL362976:GJSR-360-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:HQ_1341A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum
ProteomesiUP000001975: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303649Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ1341A.

Structurei

3D structure databases

ProteinModelPortaliQ18KI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 533195Protein kinaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 328328Kae1Add
BLAST
Regioni133 – 1375Threonylcarbamoyl-AMP bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18KI0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRILGIEGTA WAASAALYNT HDETIVIESD PYQPDSGGLH PREAAEHMST
60 70 80 90 100
ALPEVISTIL ERAVSSGNTD AIGIDAIAFS RGPGLGPCLR VVGTAARTLT
110 120 130 140 150
QALSVPLIGV NHMIAHLEIG RHQSGFTTPV CLNASGANAH LLGYHRRQYQ
160 170 180 190 200
VLGETMDTGV GNAIDKFTRH LGWNHPGGPK VEAAATDGSY HDLPYVVKGM
210 220 230 240 250
DFSFSGIMSA AKDAVDNEVP VVDVCTGLQE TIFAMLTEVA ERALSLTGSN
260 270 280 290 300
ELVLGGGVGQ NDRLREMLST MCTARGASFY APESRFLRDN AGMIAVLGAA
310 320 330 340 350
MYEAGQTISV NDSAVDPTFR PDAVTVTWRD DETSVTRTPA TLDKTPVRGA
360 370 380 390 400
EAIVRRINDH VVKRRVEKSY RHPKLDRRLR AERTRAEARL TSAARRLGVP
410 420 430 440 450
TPLIFDADPE TGTLVFEYVG ETDLAADLTV SRCHAVGQHL GRIHNAGFVH
460 470 480 490 500
GDPTTRNVRV DSAQNYLIDF GLGYHTDHVE DHAMDLHVFI QSVTGTASDP
510 520 530
APLITAFESG YAETGISTVQ SRLRDIESRG RYH
Length:533
Mass (Da):57,542
Last modified:July 25, 2006 - v1
Checksum:iA504147E944A27A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180088 Genomic DNA. Translation: CAJ51469.1.
RefSeqiWP_011570626.1. NC_008212.1.
YP_657116.1. NC_008212.1.

Genome annotation databases

EnsemblBacteriaiCAJ51469; CAJ51469; HQ_1341A.
GeneIDi4194019.
KEGGihwa:HQ1341A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180088 Genomic DNA. Translation: CAJ51469.1 .
RefSeqi WP_011570626.1. NC_008212.1.
YP_657116.1. NC_008212.1.

3D structure databases

ProteinModelPortali Q18KI0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362976.HQ1341A.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ51469 ; CAJ51469 ; HQ_1341A .
GeneIDi 4194019.
KEGGi hwa:HQ1341A.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci HWAL362976:GJSR-360-MONOMER.

Family and domain databases

HAMAPi MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
    Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
    BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16790 / HBSQ001.

Entry informationi

Entry nameiKAE1B_HALWD
AccessioniPrimary (citable) accession number: Q18KI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 25, 2006
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3