Q18KI0 (KAE1B_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

1. Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
   Alternative name(s):
   t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
2. Probable serine/threonine-protein kinase BUD32 homolog
   EC=2.7.11.1

Gene names

Ordered Locus Names:

HQ_1341A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum › Haloquadratum walsbyi

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP-Rule MF_01447
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01447.
Sequence similarities	In the N-terminal section; belongs to the KAE1 / YgjD family. In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	threonylcarbamoyladenosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447		PRO_0000303649
Regions
	Domain	339 – 533	195	Protein kinase
	Nucleotide binding	347 – 354	8	ATP By similarity
	Region	1 – 328	328	Kae1 HAMAP-Rule MF_01447
Sites
	Active site	452	1	Proton acceptor By similarity
	Binding site	363	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18KI0 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: A504147E944A27A2	FASTA	533	57,542
	10 20 30 40 50 60 MRILGIEGTA WAASAALYNT HDETIVIESD PYQPDSGGLH PREAAEHMST ALPEVISTIL 70 80 90 100 110 120 ERAVSSGNTD AIGIDAIAFS RGPGLGPCLR VVGTAARTLT QALSVPLIGV NHMIAHLEIG 130 140 150 160 170 180 RHQSGFTTPV CLNASGANAH LLGYHRRQYQ VLGETMDTGV GNAIDKFTRH LGWNHPGGPK 190 200 210 220 230 240 VEAAATDGSY HDLPYVVKGM DFSFSGIMSA AKDAVDNEVP VVDVCTGLQE TIFAMLTEVA 250 260 270 280 290 300 ERALSLTGSN ELVLGGGVGQ NDRLREMLST MCTARGASFY APESRFLRDN AGMIAVLGAA 310 320 330 340 350 360 MYEAGQTISV NDSAVDPTFR PDAVTVTWRD DETSVTRTPA TLDKTPVRGA EAIVRRINDH 370 380 390 400 410 420 VVKRRVEKSY RHPKLDRRLR AERTRAEARL TSAARRLGVP TPLIFDADPE TGTLVFEYVG 430 440 450 460 470 480 ETDLAADLTV SRCHAVGQHL GRIHNAGFVH GDPTTRNVRV DSAQNYLIDF GLGYHTDHVE 490 500 510 520 530 DHAMDLHVFI QSVTGTASDP APLITAFESG YAETGISTVQ SRLRDIESRG RYH

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ51469.1.
RefSeq	YP_657116.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18KI0.
ModBase	Search...
Protein-protein interaction databases
STRING	362976.HQ1341A.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ51469; CAJ51469; HQ_1341A.
GeneID	4194019.
KEGG	hwa:HQ1341A.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0533.
HOGENOM	HOG000109569.
KO	K15904.
OMA	GNCLDQF.
ProtClustDB	PRK09605.
Enzyme and pathway databases
BioCyc	HWAL362976:GJSR-360-MONOMER.
Family and domain databases
HAMAP	MF_01447. Kae1_Bud32_arch.
InterPro	IPR000905. Gcp-like_dom. IPR022495. Kae1-assoc_kinase_Bud32. IPR017861. KAE1/YgjD. IPR022449. Kae1_arc. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR009220. tRNA_threonyl_synthase/kinase. [Graphical view]
PANTHER	PTHR11735:SF6. PTHR11735:SF6. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PIRSF	PIRSF036401. Gcp_STYKS. 1 hit.
PRINTS	PR00789. OSIALOPTASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. arch_bud32. 1 hit. TIGR03722. arch_KAE1. 1 hit. TIGR00329. gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAE1B_HALWD

Accession

Primary (citable) accession number: Q18KI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program