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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

HQ_1341A

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi112IronUniRule annotation1
Metal bindingi116IronUniRule annotation1
Binding sitei165Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei178Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation1
Binding sitei182Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei261Threonylcarbamoyl-AMPUniRule annotation1
Metal bindingi289IronUniRule annotation1
Binding sitei363ATPUniRule annotation1
Active sitei452Proton acceptor; for kinase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi347 – 354ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Kinase, Multifunctional enzyme, Serine/threonine-protein kinase, Transferase
Biological processtRNA processing
LigandATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHWAL362976:G1G1J-358-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:HQ_1341A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloquadratum
Proteomesi
  • UP000001975 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003036491 – 533Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST533

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ1341A

Structurei

3D structure databases

ProteinModelPortaliQ18KI0
SMRiQ18KI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini339 – 533Protein kinaseUniRule annotationAdd BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 328Kae1Add BLAST328
Regioni133 – 137Threonylcarbamoyl-AMP bindingUniRule annotation5

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183 Archaea
ENOG4102TVI Archaea
COG0533 LUCA
COG3642 LUCA
HOGENOMiHOG000109569
KOiK15904
OMAiGETMDTG
OrthoDBiPOG093Z05SV

Family and domain databases

HAMAPiMF_01446 Kae1, 1 hit
MF_01447 Kae1_Bud32_arch, 1 hit
InterProiView protein in InterPro
IPR022495 Bud32
IPR000905 Gcp-like_dom
IPR034680 Kae1/OSGEP
IPR017861 KAE1/TsaD
IPR022449 Kae1_arc
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR009220 tRNA_threonyl_synthase/kinase
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF14 PTHR11735:SF14, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PIRSFiPIRSF036401 Gcp_STYKS, 1 hit
PRINTSiPR00789 OSIALOPTASE
SUPFAMiSSF56112 SSF56112, 1 hit
TIGRFAMsiTIGR03724 arch_bud32, 1 hit
TIGR03722 arch_KAE1, 1 hit
TIGR00329 gcp_kae1, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q18KI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILGIEGTA WAASAALYNT HDETIVIESD PYQPDSGGLH PREAAEHMST
60 70 80 90 100
ALPEVISTIL ERAVSSGNTD AIGIDAIAFS RGPGLGPCLR VVGTAARTLT
110 120 130 140 150
QALSVPLIGV NHMIAHLEIG RHQSGFTTPV CLNASGANAH LLGYHRRQYQ
160 170 180 190 200
VLGETMDTGV GNAIDKFTRH LGWNHPGGPK VEAAATDGSY HDLPYVVKGM
210 220 230 240 250
DFSFSGIMSA AKDAVDNEVP VVDVCTGLQE TIFAMLTEVA ERALSLTGSN
260 270 280 290 300
ELVLGGGVGQ NDRLREMLST MCTARGASFY APESRFLRDN AGMIAVLGAA
310 320 330 340 350
MYEAGQTISV NDSAVDPTFR PDAVTVTWRD DETSVTRTPA TLDKTPVRGA
360 370 380 390 400
EAIVRRINDH VVKRRVEKSY RHPKLDRRLR AERTRAEARL TSAARRLGVP
410 420 430 440 450
TPLIFDADPE TGTLVFEYVG ETDLAADLTV SRCHAVGQHL GRIHNAGFVH
460 470 480 490 500
GDPTTRNVRV DSAQNYLIDF GLGYHTDHVE DHAMDLHVFI QSVTGTASDP
510 520 530
APLITAFESG YAETGISTVQ SRLRDIESRG RYH
Length:533
Mass (Da):57,542
Last modified:July 25, 2006 - v1
Checksum:iA504147E944A27A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA Translation: CAJ51469.1
RefSeqiWP_011570626.1, NC_008212.1

Genome annotation databases

EnsemblBacteriaiCAJ51469; CAJ51469; HQ_1341A
GeneIDi4194019
KEGGihwa:HQ_1341A

Similar proteinsi

Entry informationi

Entry nameiKAE1B_HALWD
AccessioniPrimary (citable) accession number: Q18KI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health