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Q18KI0 (KAE1B_HALWD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.3.1.-
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:HQ_1341A
OrganismHaloquadratum walsbyi (strain DSM 16790 / HBSQ001) [Complete proteome] [HAMAP]
Taxonomic identifier362976 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303649

Regions

Domain339 – 533195Protein kinase
Nucleotide binding347 – 3548ATP By similarity
Region1 – 328328Kae1 HAMAP-Rule MF_01447
Region133 – 1375Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4521Proton acceptor; for kinase activity By similarity
Metal binding1121Iron By similarity
Metal binding1161Iron By similarity
Metal binding2891Iron By similarity
Binding site1651Threonylcarbamoyl-AMP By similarity
Binding site1781Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1821Threonylcarbamoyl-AMP By similarity
Binding site2611Threonylcarbamoyl-AMP By similarity
Binding site3631ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q18KI0 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: A504147E944A27A2

FASTA53357,542
        10         20         30         40         50         60 
MRILGIEGTA WAASAALYNT HDETIVIESD PYQPDSGGLH PREAAEHMST ALPEVISTIL 

        70         80         90        100        110        120 
ERAVSSGNTD AIGIDAIAFS RGPGLGPCLR VVGTAARTLT QALSVPLIGV NHMIAHLEIG 

       130        140        150        160        170        180 
RHQSGFTTPV CLNASGANAH LLGYHRRQYQ VLGETMDTGV GNAIDKFTRH LGWNHPGGPK 

       190        200        210        220        230        240 
VEAAATDGSY HDLPYVVKGM DFSFSGIMSA AKDAVDNEVP VVDVCTGLQE TIFAMLTEVA 

       250        260        270        280        290        300 
ERALSLTGSN ELVLGGGVGQ NDRLREMLST MCTARGASFY APESRFLRDN AGMIAVLGAA 

       310        320        330        340        350        360 
MYEAGQTISV NDSAVDPTFR PDAVTVTWRD DETSVTRTPA TLDKTPVRGA EAIVRRINDH 

       370        380        390        400        410        420 
VVKRRVEKSY RHPKLDRRLR AERTRAEARL TSAARRLGVP TPLIFDADPE TGTLVFEYVG 

       430        440        450        460        470        480 
ETDLAADLTV SRCHAVGQHL GRIHNAGFVH GDPTTRNVRV DSAQNYLIDF GLGYHTDHVE 

       490        500        510        520        530 
DHAMDLHVFI QSVTGTASDP APLITAFESG YAETGISTVQ SRLRDIESRG RYH 

« Hide

References

[1]"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16790 / HBSQ001.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180088 Genomic DNA. Translation: CAJ51469.1.
RefSeqYP_657116.1. NC_008212.1.

3D structure databases

ProteinModelPortalQ18KI0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362976.HQ1341A.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ51469; CAJ51469; HQ_1341A.
GeneID4194019.
KEGGhwa:HQ1341A.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.
ProtClustDBPRK09605.

Enzyme and pathway databases

BioCycHWAL362976:GJSR-360-MONOMER.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PANTHERPTHR11735:SF6. PTHR11735:SF6. 1 hit.
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_HALWD
AccessionPrimary (citable) accession number: Q18KI0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 25, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families