Q18JI5 (PURL_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	HQ1693A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050310
Regions
	Nucleotide binding	106 – 117	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q18JI5 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 762F5454492F4AF2	FASTA	725	75,726
	10 20 30 40 50 60 MTLSDSDLEL ITTELGRELT PAEAALFENL WSEHCAYRSS RPLLQSFTTG DDSANDEGAE 70 80 90 100 110 120 HVVIGPGDDA AVVRLPRVDE ASETPIYITM GIESHNHPSY VDPYDGAATG VGGIVRDTLS 130 140 150 160 170 180 MGAYPIALAD SLYFGSFDRE RTQYLLDGVV EGIADYGNAI GVPTVAGSVE FHNKYTGNPL 190 200 210 220 230 240 VNVACIGLLT DERLVTAEAQ SPGNKLVLVG NATGRDGLGG ASFASEDLSE DAETEDRPAV 250 260 270 280 290 300 QVGDPYTEKR LIEANEELIT AELIQSARDL GAAGLGGASS ELVAKGGLGA RINLERVHQR 310 320 330 340 350 360 EPAMNAMEIL LAESQERMCY EVHPDDVETV GDIAAKYDLG YAVIGEIRDG NYVCTFGDET 370 380 390 400 410 420 VVDVPAGFLA DGAPMNDLSA TEPPTPEEST FPAIDAATAF NTIISTPNTA SKRWIYRQYD 430 440 450 460 470 480 HEVGLRTAMR PGDDAALIAI REAGVGLALS SGAVPAWTES NPYKGAQAVA LENATNVAAK 490 500 510 520 530 540 GATPIAAVDC LNGGNPEKPA VYGAFKDIVQ GLADMCHTLS IPVVGGNVSL YNDSTSGPIP 550 560 570 580 590 600 PTPTLALVGT KKTFDAPPAA LTGDGTLLLV GHQSNNLGGS ELLAQIGGTD KFPALPSNPT 610 620 630 640 650 660 AVVDSLARVA NHPSTLATHD VSTGGLAVTL AELITSDAGA RVTVQNKTSL FTETPGRAVI 670 680 690 700 710 720 ETTDPDAVRH IFEGVAPVVS LGDTTTDGTL TINIDDEDTA NESPAVTTDA ATVAKQRRIL EDALE

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed: 16820047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ51821.1.
RefSeq	YP_657461.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18JI5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18JI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4194536.
GenomeReviews	Gene locus HQ1693A in contig AM180088_GR.
KEGG	hwa:HQ1693A.
NMPDR	fig|362976.6.peg.700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q18JI5.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	HWAL362976:HQ1693A-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_HALWD

Accession

Primary (citable) accession number: Q18JI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program