Q18JG3 (SYP_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	HQ1715A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Proline--tRNA ligase HAMAP MF_01571		PRO_0000288418

Sequences

	Sequence		Length	Mass (Da)
	Q18JG3 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 6987029E985A1681	FASTA	512	57,527
	10 20 30 40 50 60 MSEDQDLGIT QSKIHNTGEW YAEVVQKAEL ANYGPEGMSG FIVTRPRAYG LWERVQSYLD 70 80 90 100 110 120 TRFKQTGVQN AYFPLFIPEG YLEREKEIVE GFDPEVAWVE QAGRNELEER LAVRPTSESI 130 140 150 160 170 180 IAPYLSQWIR SYRDLPLRVN QWTSVVRWEA TETKPFFRTK EFLWQEGHTA HATRADAWAE 190 200 210 220 230 240 TMLRLNQYES TYEDLLAIPV LQGAKPEHDK FPGADTTTTV EALMPDGKSV QGATSHYLGT 250 260 270 280 290 300 EFADAFDITY TDTDETSRVA HTTSWGLSWR ALGALIMTHS DNQGLVLPPT VAPEQVVIVP 310 320 330 340 350 360 IWQTETKERV LEYAEDVANN LDDAGIRVEL DDRDDQNPGF KFNEWELKGV PLRAEIGPDE 370 380 390 400 410 420 ATEGTVTLIH RPDGESITAE RSEIVETVQE QFDAVYAKLY AAAEETLNSN IRIAETRSEL 430 440 450 460 470 480 LGTIGQHGGY VKTPWCGDEG CETAIKDEIA AEIVMVPISS DEDDEQDTTD ENMGVNNDTT 490 500 510 VESNEKSLDL TDSTCVVCDN PAFKTAYFAK SY

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed: 16820047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ51843.1.
RefSeq	YP_657483.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18JG3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18JG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4194509.
GenomeReviews	Gene locus HQ1715A in contig AM180088_GR.
KEGG	hwa:HQ1715A.
NMPDR	fig|362976.6.peg.722.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	YSKWIRG.
PhylomeDB	Q18JG3.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	HWAL362976:HQ1715A-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 2 hits.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_HALWD

Accession

Primary (citable) accession number: Q18JG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program