Q18HT7 (DNLI1_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase 1
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 1

Gene names

Name:	lig1
Ordered Locus Names:	HQ2327A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	DNA ligase 1 HAMAP MF_00407		PRO_0000365246
Sites
	Active site	274	1	N6-AMP-lysine intermediate By similarity
	Binding site	272	1	ATP By similarity
	Binding site	279	1	ATP By similarity
	Binding site	294	1	ATP By similarity
	Binding site	324	1	ATP By similarity
	Binding site	367	1	ATP By similarity
	Binding site	472	1	ATP By similarity
	Binding site	478	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18HT7 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 8251D00071A1C56F	FASTA	618	68,163
	10 20 30 40 50 60 MKYSELAEVY TDVAATSATT QKETLLADCF LDVYDNTETS LLPVTVRLVR GRVFARWEAA 70 80 90 100 110 120 EIGVSTAGAA DAISKATGVE SEQIETWWRE EGDLGDAAAY AVDDRVQQTL TATPLTVTRV 130 140 150 160 170 180 YETLREVASY EGSGSQQKQR DEIAGLISDA SPLAARYIVR TVTGSMRLGV GEGIVRNALA 190 200 210 220 230 240 SAFLDMSDDA ADAVQRAIDL TNDVAVVAQR VAEQGQPGLD DVDLELFRPI KPMLAKQAES 250 260 270 280 290 300 IQTAVDELAA DPKHIERNHN IELSHSGAVL IEFKYDGIRA KIHRDGDDIR VYTRRLSDVT 310 320 330 340 350 360 EQFPDVVSAA RDHIDAKHYI IEAELVGYDP QSNDDTPVTF QTLAQRIQRT DNVATIAKSI 370 380 390 400 410 420 PVTTYVFDLL ALNGESLLTD TLEMRLERLN TILSTKATSD SKSASTHNAH SSTSVTTVSS 430 440 450 460 470 480 DQYQSSSSLR RAQCTWATTQ TTAAEFYEDA LAAGHEGVMI KNPARPYQPG SRVGYQRKIK 490 500 510 520 530 540 PTMEPLDLVV TRAKWSEGRK SDFLGRPYLA CRTADGNLAE VGRMHTGFTD DQLETFTNRV 550 560 570 580 590 600 EPLIENVEGR EATLTPAVVL AVEYEEIQIS STYDSGYALR FPRLKQIRHD CDPSQADSLE 610 RVERLYEQQS LDESQTSV

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed: 16820047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ52448.1.
RefSeq	YP_658064.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18HT7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18HT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4194189.
GenomeReviews	Gene locus HQ2327A in contig AM180088_GR.
KEGG	hwa:HQ2327A.
NMPDR	fig|362976.6.peg.1342.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04596.
HOGENOM	HBG570821.
PhylomeDB	Q18HT7.
Enzyme and pathway databases
BioCyc	HWAL362976:HQ2327A-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI1_HALWD

Accession

Primary (citable) accession number: Q18HT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	July 25, 2006
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program