Q18GX5 (DNLI2_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase 2
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 2

Gene names

Name:	lig2
Ordered Locus Names:	HQ_2659A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum › Haloquadratum walsbyi

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	DNA ligase 2 HAMAP-Rule MF_00407		PRO_0000365247
Sites
	Active site	314	1	N6-AMP-lysine intermediate By similarity
	Binding site	312	1	ATP By similarity
	Binding site	319	1	ATP By similarity
	Binding site	334	1	ATP By similarity
	Binding site	363	1	ATP By similarity
	Binding site	403	1	ATP By similarity
	Binding site	476	1	ATP By similarity
	Binding site	482	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18GX5 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: CF6C72C63A49F560	FASTA	618	66,924
	10 20 30 40 50 60 MQFDDFANHV ITIEAESADL EITSLVSKLL TTAGNMDDKT LSTVVRFIKG TVFPAWSSQT 70 80 90 100 110 120 LDIGPALLHT TIAQAAGPNV DSADVENRLA EYGEIGAVAA SYDLNGQQGL SAFTPSTPDS 130 140 150 160 170 180 LTVGHVDKTL RAVASASGDG SESRRRNLLF GLFSQASSSE ARVLARLILG EMRIGVGTGT 190 200 210 220 230 240 VRDAIISGFI EPAADADKKT LESREDAKSV PPASQPEITN KISGDTSPNT SESVQTKKSD 250 260 270 280 290 300 PDTSSNVDPS AVVERALQVS NDYGMVATIA RNQGQAGLID ISLELGRPIQ AMLAQAADGV 310 320 330 340 350 360 DAVDTWDAVA IETKFDGARV QIHTDGESVS LYSRNMEDVT DPLPEIVEFI SQKVTVPAIL 370 380 390 400 410 420 DAEVVAVSDD GDPLAFQEVL RRFRRKYDID AMRESVNLNV YVFDCLHIDD ADLLDIPLRE 430 440 450 460 470 480 RREKLRELFN TTDALSPFQL TTDPNVIAQA RSKALSNGHE GVMLKDPDST YNPGSRGQHW 490 500 510 520 530 540 LKHKPDVETL DLVVTGAEWG EGRRANVFGT FVVSARTTAD EQQFNSLGKV ATGLTDDQLT 550 560 570 580 590 600 TLTEQLRPHV RSEDGQTVMI EPAIVVEVGY EEIQRSPTYD SGFALRFPRV VGIRHDKAIS 610 DVDSLSRIKH LTTGESPD

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ52770.1.
RefSeq	YP_658376.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18GX5.
ModBase	Search...
Protein-protein interaction databases
STRING	362976.HQ2659A.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ52770; CAJ52770; HQ_2659A.
GeneID	4194190.
KEGG	hwa:HQ2659A.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1793.
HOGENOM	HOG000036008.
KO	K10747.
OMA	ARVQVHK.
ProtClustDB	CLSK511253.
Enzyme and pathway databases
BioCyc	HWAL362976:GJSR-1760-MONOMER.
Family and domain databases
Gene3D	1.10.3260.10. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_00407. DNA_ligase.
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI2_HALWD

Accession

Primary (citable) accession number: Q18GX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program