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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei609ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHWAL362976:G1G1J-1738-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HQ_2671A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloquadratum
Proteomesi
  • UP000001975 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221491 – 1059Isoleucine--tRNA ligaseAdd BLAST1059

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ2671A

Structurei

3D structure databases

ProteinModelPortaliQ18GW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi606 – 610"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q18GW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEVDDQYTP ADVETAIETY WDDTNAYEAT KEAHADDPSF FFVDGPPYTS
60 70 80 90 100
GQMHLGTAWN KTLKDAIIRY KRMTGHHVTD RPGYDMHGLP IEVKVEEELG
110 120 130 140 150
FETKRDIEEY GMESFIEECK RFAVDNRKAM DEDFQSIGVW MDWDNPYETL
160 170 180 190 200
SPEYMEAAWW AFQQVDDRGL VERGKRSVSY CPRCQTAIAA NEVEYDEITS
210 220 230 240 250
PSIYVRFPLS NKEGSLVIWT TTPWTIPANT FVAVDKDLTY QAVRAEQGDD
260 270 280 290 300
SEVLYIAESC VEDVLKQGRY DDYTVVEEYS GDELTGWEYD HPLADQVQTY
310 320 330 340 350
ADFAGAGEVY TAEYVEADRT GLVHSAPGHG QEDFARGQEL DLETFVPVDG
360 370 380 390 400
RGEFTEAAGQ YTGTFVRDAN DEIINDLDEE GVLLSSGTHE HRYGHCWRCD
410 420 430 440 450
TDIIFLATDQ WFITVTDIKD ELLDNINDSE WYPQWARDNR FRDFVADAPD
460 470 480 490 500
WNVSRQRYWG IPLPIWESVD DADTGDNSTS DDWIVIGTRE ELAERADQDV
510 520 530 540 550
NPAEIDLHRP AVDPLTITEN GSRYERVPDV FDVWIDSSVA SWGTIDYPGE
560 570 580 590 600
TDAYDELWPA DFIVEAHDQT RGWFWSQLGM GTAATGQVPY EEVMMHGFAN
610 620 630 640 650
DENGRKMSKS RGNIVTPEEA IDRAGRDPLR AYLLSHDQQG VDLSFEWDGL
660 670 680 690 700
GEMQSTLNIF WNVFRFPLPY MDLDGYDPAT ADLSEGSMNI VDEWVLSRLQ
710 720 730 740 750
SVKATTRAAW EEYEIDTAVN TILEFITDDV SRFYIKAIRE RMWADEDSAS
760 770 780 790 800
KRGAYATLST VLDETIRLLA PIAPYLTEQM YQHLNGSETT VHALSYPSVD
810 820 830 840 850
PEWQNETLES EMAVLRDVEE AAANARQQGG RKLRWPVPRV IVEAEDDSIA
860 870 880 890 900
DAVESLSGLL ADRVNTEAIE TVTQFDELIE RAQPEMSVIG PEFGADAQRV
910 920 930 940 950
MDAIEGGSRE ILTEGVTIDG VQYEITDEMI TFDAEPPAYI SAADFDGGTV
960 970 980 990 1000
YVDTSLTESI EAEGYARDVI RRIQQMRKEL ALDVDTEIQT AVDVADDRVA
1010 1020 1030 1040 1050
DLVAQQRDVV ATETRTNAFV DNIDRASENG QALIEEWDVE GVTVTIGVAP

LKAQLSDQS
Length:1,059
Mass (Da):119,617
Last modified:July 25, 2006 - v1
Checksum:iE2353B1F064CE233
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA Translation: CAJ52782.1
RefSeqiWP_011571898.1, NC_008212.1

Genome annotation databases

EnsemblBacteriaiCAJ52782; CAJ52782; HQ_2671A
GeneIDi4194153
KEGGihwa:HQ_2671A

Similar proteinsi

Entry informationi

Entry nameiSYI_HALWD
AccessioniPrimary (citable) accession number: Q18GW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health