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Q18GW3

- SYI_HALWD

UniProt

Q18GW3 - SYI_HALWD

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Protein
Isoleucine--tRNA ligase
Gene
ileS, HQ_2671A
Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei609 – 6091ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHWAL362976:GJSR-1772-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HQ_2671A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum
ProteomesiUP000001975: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10591059Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022149Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ2671A.

Structurei

3D structure databases

ProteinModelPortaliQ18GW3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotation
Add
BLAST
Motifi606 – 6105"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRYVEGKW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18GW3-1 [UniParc]FASTAAdd to Basket

« Hide

MDEVDDQYTP ADVETAIETY WDDTNAYEAT KEAHADDPSF FFVDGPPYTS     50
GQMHLGTAWN KTLKDAIIRY KRMTGHHVTD RPGYDMHGLP IEVKVEEELG 100
FETKRDIEEY GMESFIEECK RFAVDNRKAM DEDFQSIGVW MDWDNPYETL 150
SPEYMEAAWW AFQQVDDRGL VERGKRSVSY CPRCQTAIAA NEVEYDEITS 200
PSIYVRFPLS NKEGSLVIWT TTPWTIPANT FVAVDKDLTY QAVRAEQGDD 250
SEVLYIAESC VEDVLKQGRY DDYTVVEEYS GDELTGWEYD HPLADQVQTY 300
ADFAGAGEVY TAEYVEADRT GLVHSAPGHG QEDFARGQEL DLETFVPVDG 350
RGEFTEAAGQ YTGTFVRDAN DEIINDLDEE GVLLSSGTHE HRYGHCWRCD 400
TDIIFLATDQ WFITVTDIKD ELLDNINDSE WYPQWARDNR FRDFVADAPD 450
WNVSRQRYWG IPLPIWESVD DADTGDNSTS DDWIVIGTRE ELAERADQDV 500
NPAEIDLHRP AVDPLTITEN GSRYERVPDV FDVWIDSSVA SWGTIDYPGE 550
TDAYDELWPA DFIVEAHDQT RGWFWSQLGM GTAATGQVPY EEVMMHGFAN 600
DENGRKMSKS RGNIVTPEEA IDRAGRDPLR AYLLSHDQQG VDLSFEWDGL 650
GEMQSTLNIF WNVFRFPLPY MDLDGYDPAT ADLSEGSMNI VDEWVLSRLQ 700
SVKATTRAAW EEYEIDTAVN TILEFITDDV SRFYIKAIRE RMWADEDSAS 750
KRGAYATLST VLDETIRLLA PIAPYLTEQM YQHLNGSETT VHALSYPSVD 800
PEWQNETLES EMAVLRDVEE AAANARQQGG RKLRWPVPRV IVEAEDDSIA 850
DAVESLSGLL ADRVNTEAIE TVTQFDELIE RAQPEMSVIG PEFGADAQRV 900
MDAIEGGSRE ILTEGVTIDG VQYEITDEMI TFDAEPPAYI SAADFDGGTV 950
YVDTSLTESI EAEGYARDVI RRIQQMRKEL ALDVDTEIQT AVDVADDRVA 1000
DLVAQQRDVV ATETRTNAFV DNIDRASENG QALIEEWDVE GVTVTIGVAP 1050
LKAQLSDQS 1059
Length:1,059
Mass (Da):119,617
Last modified:July 25, 2006 - v1
Checksum:iE2353B1F064CE233
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180088 Genomic DNA. Translation: CAJ52782.1.
RefSeqiWP_011571898.1. NC_008212.1.
YP_658388.1. NC_008212.1.

Genome annotation databases

EnsemblBacteriaiCAJ52782; CAJ52782; HQ_2671A.
GeneIDi4194153.
KEGGihwa:HQ2671A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180088 Genomic DNA. Translation: CAJ52782.1 .
RefSeqi WP_011571898.1. NC_008212.1.
YP_658388.1. NC_008212.1.

3D structure databases

ProteinModelPortali Q18GW3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362976.HQ2671A.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ52782 ; CAJ52782 ; HQ_2671A .
GeneIDi 4194153.
KEGGi hwa:HQ2671A.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RYVEGKW.

Enzyme and pathway databases

BioCyci HWAL362976:GJSR-1772-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
    Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
    BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16790 / HBSQ001.

Entry informationi

Entry nameiSYI_HALWD
AccessioniPrimary (citable) accession number: Q18GW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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