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Q18GW3

- SYI_HALWD

UniProt

Q18GW3 - SYI_HALWD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei609 – 6091ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHWAL362976:GJSR-1772-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:HQ_2671A
    OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
    Taxonomic identifieri362976 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum
    ProteomesiUP000001975: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10591059Isoleucine--tRNA ligasePRO_1000022149Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi362976.HQ2671A.

    Structurei

    3D structure databases

    ProteinModelPortaliQ18GW3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi606 – 6105"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRYVEGKW.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q18GW3-1 [UniParc]FASTAAdd to Basket

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    MDEVDDQYTP ADVETAIETY WDDTNAYEAT KEAHADDPSF FFVDGPPYTS     50
    GQMHLGTAWN KTLKDAIIRY KRMTGHHVTD RPGYDMHGLP IEVKVEEELG 100
    FETKRDIEEY GMESFIEECK RFAVDNRKAM DEDFQSIGVW MDWDNPYETL 150
    SPEYMEAAWW AFQQVDDRGL VERGKRSVSY CPRCQTAIAA NEVEYDEITS 200
    PSIYVRFPLS NKEGSLVIWT TTPWTIPANT FVAVDKDLTY QAVRAEQGDD 250
    SEVLYIAESC VEDVLKQGRY DDYTVVEEYS GDELTGWEYD HPLADQVQTY 300
    ADFAGAGEVY TAEYVEADRT GLVHSAPGHG QEDFARGQEL DLETFVPVDG 350
    RGEFTEAAGQ YTGTFVRDAN DEIINDLDEE GVLLSSGTHE HRYGHCWRCD 400
    TDIIFLATDQ WFITVTDIKD ELLDNINDSE WYPQWARDNR FRDFVADAPD 450
    WNVSRQRYWG IPLPIWESVD DADTGDNSTS DDWIVIGTRE ELAERADQDV 500
    NPAEIDLHRP AVDPLTITEN GSRYERVPDV FDVWIDSSVA SWGTIDYPGE 550
    TDAYDELWPA DFIVEAHDQT RGWFWSQLGM GTAATGQVPY EEVMMHGFAN 600
    DENGRKMSKS RGNIVTPEEA IDRAGRDPLR AYLLSHDQQG VDLSFEWDGL 650
    GEMQSTLNIF WNVFRFPLPY MDLDGYDPAT ADLSEGSMNI VDEWVLSRLQ 700
    SVKATTRAAW EEYEIDTAVN TILEFITDDV SRFYIKAIRE RMWADEDSAS 750
    KRGAYATLST VLDETIRLLA PIAPYLTEQM YQHLNGSETT VHALSYPSVD 800
    PEWQNETLES EMAVLRDVEE AAANARQQGG RKLRWPVPRV IVEAEDDSIA 850
    DAVESLSGLL ADRVNTEAIE TVTQFDELIE RAQPEMSVIG PEFGADAQRV 900
    MDAIEGGSRE ILTEGVTIDG VQYEITDEMI TFDAEPPAYI SAADFDGGTV 950
    YVDTSLTESI EAEGYARDVI RRIQQMRKEL ALDVDTEIQT AVDVADDRVA 1000
    DLVAQQRDVV ATETRTNAFV DNIDRASENG QALIEEWDVE GVTVTIGVAP 1050
    LKAQLSDQS 1059
    Length:1,059
    Mass (Da):119,617
    Last modified:July 25, 2006 - v1
    Checksum:iE2353B1F064CE233
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180088 Genomic DNA. Translation: CAJ52782.1.
    RefSeqiWP_011571898.1. NC_008212.1.
    YP_658388.1. NC_008212.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ52782; CAJ52782; HQ_2671A.
    GeneIDi4194153.
    KEGGihwa:HQ2671A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180088 Genomic DNA. Translation: CAJ52782.1 .
    RefSeqi WP_011571898.1. NC_008212.1.
    YP_658388.1. NC_008212.1.

    3D structure databases

    ProteinModelPortali Q18GW3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 362976.HQ2671A.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ52782 ; CAJ52782 ; HQ_2671A .
    GeneIDi 4194153.
    KEGGi hwa:HQ2671A.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RYVEGKW.

    Enzyme and pathway databases

    BioCyci HWAL362976:GJSR-1772-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
      Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
      BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 16790 / HBSQ001.

    Entry informationi

    Entry nameiSYI_HALWD
    AccessioniPrimary (citable) accession number: Q18GW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3