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Q18GW3

- SYI_HALWD

UniProt

Q18GW3 - SYI_HALWD

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei609 – 6091ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHWAL362976:GJSR-1772-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HQ_2671A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum
ProteomesiUP000001975: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10591059Isoleucine--tRNA ligasePRO_1000022149Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ2671A.

Structurei

3D structure databases

ProteinModelPortaliQ18GW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRYVEGKW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18GW3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDEVDDQYTP ADVETAIETY WDDTNAYEAT KEAHADDPSF FFVDGPPYTS
60 70 80 90 100
GQMHLGTAWN KTLKDAIIRY KRMTGHHVTD RPGYDMHGLP IEVKVEEELG
110 120 130 140 150
FETKRDIEEY GMESFIEECK RFAVDNRKAM DEDFQSIGVW MDWDNPYETL
160 170 180 190 200
SPEYMEAAWW AFQQVDDRGL VERGKRSVSY CPRCQTAIAA NEVEYDEITS
210 220 230 240 250
PSIYVRFPLS NKEGSLVIWT TTPWTIPANT FVAVDKDLTY QAVRAEQGDD
260 270 280 290 300
SEVLYIAESC VEDVLKQGRY DDYTVVEEYS GDELTGWEYD HPLADQVQTY
310 320 330 340 350
ADFAGAGEVY TAEYVEADRT GLVHSAPGHG QEDFARGQEL DLETFVPVDG
360 370 380 390 400
RGEFTEAAGQ YTGTFVRDAN DEIINDLDEE GVLLSSGTHE HRYGHCWRCD
410 420 430 440 450
TDIIFLATDQ WFITVTDIKD ELLDNINDSE WYPQWARDNR FRDFVADAPD
460 470 480 490 500
WNVSRQRYWG IPLPIWESVD DADTGDNSTS DDWIVIGTRE ELAERADQDV
510 520 530 540 550
NPAEIDLHRP AVDPLTITEN GSRYERVPDV FDVWIDSSVA SWGTIDYPGE
560 570 580 590 600
TDAYDELWPA DFIVEAHDQT RGWFWSQLGM GTAATGQVPY EEVMMHGFAN
610 620 630 640 650
DENGRKMSKS RGNIVTPEEA IDRAGRDPLR AYLLSHDQQG VDLSFEWDGL
660 670 680 690 700
GEMQSTLNIF WNVFRFPLPY MDLDGYDPAT ADLSEGSMNI VDEWVLSRLQ
710 720 730 740 750
SVKATTRAAW EEYEIDTAVN TILEFITDDV SRFYIKAIRE RMWADEDSAS
760 770 780 790 800
KRGAYATLST VLDETIRLLA PIAPYLTEQM YQHLNGSETT VHALSYPSVD
810 820 830 840 850
PEWQNETLES EMAVLRDVEE AAANARQQGG RKLRWPVPRV IVEAEDDSIA
860 870 880 890 900
DAVESLSGLL ADRVNTEAIE TVTQFDELIE RAQPEMSVIG PEFGADAQRV
910 920 930 940 950
MDAIEGGSRE ILTEGVTIDG VQYEITDEMI TFDAEPPAYI SAADFDGGTV
960 970 980 990 1000
YVDTSLTESI EAEGYARDVI RRIQQMRKEL ALDVDTEIQT AVDVADDRVA
1010 1020 1030 1040 1050
DLVAQQRDVV ATETRTNAFV DNIDRASENG QALIEEWDVE GVTVTIGVAP

LKAQLSDQS
Length:1,059
Mass (Da):119,617
Last modified:July 25, 2006 - v1
Checksum:iE2353B1F064CE233
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA. Translation: CAJ52782.1.
RefSeqiWP_011571898.1. NC_008212.1.
YP_658388.1. NC_008212.1.

Genome annotation databases

EnsemblBacteriaiCAJ52782; CAJ52782; HQ_2671A.
GeneIDi4194153.
KEGGihwa:HQ2671A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA. Translation: CAJ52782.1 .
RefSeqi WP_011571898.1. NC_008212.1.
YP_658388.1. NC_008212.1.

3D structure databases

ProteinModelPortali Q18GW3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362976.HQ2671A.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ52782 ; CAJ52782 ; HQ_2671A .
GeneIDi 4194153.
KEGGi hwa:HQ2671A.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RYVEGKW.

Enzyme and pathway databases

BioCyci HWAL362976:GJSR-1772-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
    Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
    BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16790 / HBSQ001.

Entry informationi

Entry nameiSYI_HALWD
AccessioniPrimary (citable) accession number: Q18GW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3