Q18FC4 (RIFK_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	HQ3236A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Riboflavin kinase HAMAP MF_01285		PRO_0000322086
Regions
	Nucleotide binding	108 – 113	6	CDP By similarity
	Nucleotide binding	212 – 215	4	CDP By similarity
	Region	1 – 98	98	H-T-H motif-like HAMAP MF_01285
	Region	99 – 234	136	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	137	1	Magnesium By similarity
	Metal binding	139	1	Magnesium By similarity
	Binding site	199	1	FMN By similarity
	Binding site	207	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18FC4 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 42FF0A066A196558	FASTA	234	25,870
	10 20 30 40 50 60 MAESTTAVGH DELAALKFVA LEGAHSEPIK ISCSELSDRL DASSQTASRR LQRLEAAGYL 70 80 90 100 110 120 DRDVVTDGQW VSLTKAGETA LHKEYTQYQE IFGDNSSVVE LTGTVTSGMG EGRHYISLSG 130 140 150 160 170 180 YMKQFRERLG YEPFPGTLNI DLDDDSTRTR VAVSSLTGIQ IDGWEDDERT FGPATCYPAE 190 200 210 220 230 IILAEQTAEA AHIIVPERTH HDETQLEVIA PERLRDSLEL TDGQRITVQL KPKE

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed: 16820047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ53334.1.
RefSeq	YP_658929.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18FC4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18FC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4193356.
GenomeReviews	Gene locus HQ3236A in contig AM180088_GR.
KEGG	hwa:HQ3236A.
NMPDR	fig|362976.6.peg.2244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK511620.
Enzyme and pathway databases
BioCyc	HWAL362976:HQ3236A-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_HALWD

Accession

Primary (citable) accession number: Q18FC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 25, 2006
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program