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Q18ER3 (DP2L_HALWD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase II large subunit

Short name=Pol II
EC=2.7.7.7

Cleaved into the following 2 chains:

  1. Hwa polC 1 intein
    Alternative name(s):
    Hwa pol II 1 intein
  2. Hwa polC 2 intein
    Alternative name(s):
    Hwa pol II 2 intein
Gene names
Name:polC
Synonyms:polA2
Ordered Locus Names:HQ_3461A
OrganismHaloquadratum walsbyi (strain DSM 16790 / HBSQ001) [Complete proteome] [HAMAP]
Taxonomic identifier362976 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum

Protein attributes

Sequence length2289 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP-Rule MF_00324

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_00324

Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP-Rule MF_00324

Subunit structure

Heterodimer of a large subunit and a small subunit By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00324

Sequence similarities

Belongs to the archaeal DNA polymerase II family.

Contains 2 DOD-type homing endonuclease domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 966966DNA polymerase II large subunit, 1st part Potential
PRO_0000294680
Chain967 – 1495529Hwa polC 1 intein Potential
PRO_0000294681
Chain1496 – 152429DNA polymerase II large subunit, 2nd part Potential
PRO_0000294682
Chain1525 – 2068544Hwa polC 2 intein Potential
PRO_0000294683
Chain2069 – 2289221DNA polymerase II large subunit, 3rd part Potential
PRO_0000294684

Regions

Domain1222 – 1367146DOD-type homing endonuclease 1
Domain1755 – 1911157DOD-type homing endonuclease 2

Sequences

Sequence LengthMass (Da)Tools
Q18ER3 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: A5E70294203AFF05

FASTA2,289255,812
        10         20         30         40         50         60 
MRDADKQYFE TLESHLEQAF ARARQAKGQG YDPKPEVEIP VARDMADRVE NILAIPDVAE 

        70         80         90        100        110        120 
RIRELDDERS REEVALELVT DFVEGTVGDY DTREGKIEGA VRTAVALLTE GVVAAPIEGI 

       130        140        150        160        170        180 
DRVEILSDDD GSEFVNVYYA GPIRSAGGTA QALSVLVADY ARSLLDIDEY SARTDETERY 

       190        200        210        220        230        240 
AEEVSLYDRE TGLQYSPKDK ETKFITKHMP IMLDGEATGN EEVSGYRDLE RVDTNAARGG 

       250        260        270        280        290        300 
MCLVLAEGIA LKAPKIQRYT RQLAEVEWPW LQDLIDDTIG SDEHSNNSVK NGEADIVKTD 

       310        320        330        340        350        360 
KDTNESETED GIDNDDYNDS GLEPANSPRA DATNKYLRDL IAGRPVFGHP SAAGAFRLRY 

       370        380        390        400        410        420 
GRARNHGFAT AGVHPATMHI VDDFIATGTQ LKTERPGKAG GVVPVDSIKG PTVRLANGDV 

       430        440        450        460        470        480 
RCINDPEEAE KLQNGVEKIL DLGEYLVNFG EFIENNHPLA PAAYVFEWWI QEFEASNADV 

       490        500        510        520        530        540 
QALRDDPTVD LESPTFENAM RWAKKHDIPL HPAYTYLWHD ISVTEFDHLA DAVAAGEITM 

       550        560        570        580        590        600 
NEVSDTNSAS GNTSLRANTN TDDTLTIDTT PAIRETMERL LIEHHQDSDS IHIPAWRALA 

       610        620        630        640        650        660 
LSLGIKIESD NDTGIGDRMW SLTDLSKHAR KQDDGKSAIA AVNEVAPFQV RERAPTRIGS 

       670        680        690        700        710        720 
RMGRPEKSED RDLSPAVHTL FPIGEAGGNQ RNLSDAAQSF GDNTERGQIS VQLGKRRCPY 

       730        740        750        760        770        780 
CETVGFELQC AECGRHTEPQ FVCRECESVL SPDESGRVHC DRCERDVTSA EWQDIDLHQR 

       790        800        810        820        830        840 
YRDALDRVDE RESSFEILKG VKGLTSSNKT PEPLEKGILR AKHGVSSFKD GTVRYDMTDL 

       850        860        870        880        890        900 
PVTAVRPKEL DVTAAHFREL GYQTDINGNP LQFDDQLVEL KVQDIVLPDG AAEHMMKTAD 

       910        920        930        940        950        960 
FVDDLLEQFY ELNQFYQIDE RDDLIGELVF GMAPHTSAAV VGRIVGFTSA AVGYAHPYFH 

       970        980        990       1000       1010       1020 
AAKRRNCFHP ETKIWFRDTD NEWHHETIQT FVEDRLDDPE IDAVGTLVQE VDDNTDREIS 

      1030       1040       1050       1060       1070       1080 
VPSIDDNGNE RLQSVTAVSK HRAPNHLVQI ETISGREITI TPDHEMHIFE QGNLVSKQAS 

      1090       1100       1110       1120       1130       1140 
KITSGEYAVI PKRLQTISPS SHTPQHDLLR EFLTRDELTA DRLIIHTSDP VRLCNRVFPE 

      1150       1160       1170       1180       1190       1200 
EVTSCKDAVE IMQNTACHLD KNKETLIGWL GEGRIPVALL RGFVETDEAL LMSIPDDVQI 

      1210       1220       1230       1240       1250       1260 
GLRGEKVRID RHIGFTEELT SLLGYYAAAG IVHTQTNPIS YESAQQEQSR ITFYNIDTQT 

      1270       1280       1290       1300       1310       1320 
QTDLLNALNS VFEIEPIQYN LDGEILGVPG ELIRRVFDTV FDVGTQPSHK RIPQALFDAS 

      1330       1340       1350       1360       1370       1380 
ESHITSYLRC FFSTHDSLTT DTRDISATTV SREFKEDIIA ALRRLGITAE VTTQQSRSVP 

      1390       1400       1410       1420       1430       1440 
EVLPDWYAID DITHHDADNS LNLTRSYVIS IASSDAVTLQ RDRQAQEQIK YDAQGLIANN 

      1450       1460       1470       1480       1490       1500 
NAIHQSRQVT DGGRKDYITE PITDIEYVDA DIDYTYCLTV SETHSLIVND LSQKQCDGDE 

      1510       1520       1530       1540       1550       1560 
DCVMLLMDGL LNFSREYLPD KRGGSVAADS RLVAVSPDDK IVFTTIEDFW KKLNTPIERN 

      1570       1580       1590       1600       1610       1620 
GKFRKRTCVS EGWQTYAFDE NHEASLRPIE KAIRYTADES EQLRRITTQL GRSLDITDEH 

      1630       1640       1650       1660       1670       1680 
SLFRYDDGIE EVAGDDLTAG DIIVAPRTLD VEVTQTTLDL SEYIHDNERC PSEQTGSGEL 

      1690       1700       1710       1720       1730       1740 
NLASKSAISD SRNKETPGVT HNILPQRSKF TDEMTTLSPT AVGGLESEQN ETLRVGESTG 

      1750       1760       1770       1780       1790       1800 
AIERYINVDD SFGWLLGQFI AQRSISTDAL TMTVHTAAEK HAERIVATSD SVFGIKPTVN 

      1810       1820       1830       1840       1850       1860 
SIERGYEIVF PSVFDTIVSG LTAKEQSEPE QDVDHTHTDE IGIPECILHA PDDIVLSFLQ 

      1870       1880       1890       1900       1910       1920 
GFILAENAQR KGNAASEASE MVSESETTVT LETPSVGVKD GLVFLCHRLG VITDISEKSG 

      1930       1940       1950       1960       1970       1980 
EEYSVHFEES RYTVSIATEG KTNPLDQILN GERPTMPEGV SVPVPDALLT IHESIANSPH 

      1990       2000       2010       2020       2030       2040 
IDQVIPDTVV QQETVSLETL QSLLTGLSTV DLPAQLEAKR DELTLLTEGD LSYLRVESVE 

      2050       2060       2070       2080       2090       2100 
CVDYDGYLYD LQVGGEPVFT ANWLYAHNSM DAPLVMSSRI DPSEIDDEAH NMDIVRQYPR 

      2110       2120       2130       2140       2150       2160 
EFYEATRRME DPDEWEEEVT IAEEYLDTDN EYTGFNHTHD TTDIAAGPDL SAYKTLDSMM 

      2170       2180       2190       2200       2210       2220 
DKMDAQLELA RKLRAVDETD VAERVIEYHF LPDLIGNLRA FSRQQTRCLD CGESYRRMPL 

      2230       2240       2250       2260       2270       2280 
TGECRECGGR VNLTVHEGSV NKYMDTAIHI AEEFDCREYT KQRLEVLERS LESIFEDDTN 


KQSGIADFM 

« Hide

References

[1]"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16790 / HBSQ001.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180088 Genomic DNA. Translation: CAJ53557.1.
RefSeqYP_659142.1. NC_008212.1.

3D structure databases

ProteinModelPortalQ18ER3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362976.HQ3461A.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ53557; CAJ53557; HQ_3461A.
GeneID4194482.
KEGGhwa:HQ3461A.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1933.
KOK02322.
OMAEGYARES.

Enzyme and pathway databases

BioCycHWAL362976:GJSR-2597-MONOMER.

Family and domain databases

Gene3D3.10.28.10. 2 hits.
HAMAPMF_00324. DNApol_II_L_arch.
InterProIPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamPF03833. PolC_DP2. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
[Graphical view]
SUPFAMSSF55608. SSF55608. 2 hits.
TIGRFAMsTIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEPS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDP2L_HALWD
AccessionPrimary (citable) accession number: Q18ER3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries