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Q18ER3

- DP2L_HALWD

UniProt

Q18ER3 - DP2L_HALWD

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Protein

DNA polymerase II large subunit

Gene

polC

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. exonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA catabolic process Source: InterPro
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciHWAL362976:GJSR-2597-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IIUniRule annotation
Cleaved into the following 2 chains:
Alternative name(s):
Hwa pol II 1 intein
Alternative name(s):
Hwa pol II 2 intein
Gene namesi
Name:polCUniRule annotation
Synonyms:polA2
Ordered Locus Names:HQ_3461A
OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Taxonomic identifieri362976 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum
ProteomesiUP000001975: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966DNA polymerase II large subunit, 1st partSequence AnalysisPRO_0000294680Add
BLAST
Chaini967 – 1495529Hwa polC 1 inteinSequence AnalysisPRO_0000294681Add
BLAST
Chaini1496 – 152429DNA polymerase II large subunit, 2nd partSequence AnalysisPRO_0000294682Add
BLAST
Chaini1525 – 2068544Hwa polC 2 inteinSequence AnalysisPRO_0000294683Add
BLAST
Chaini2069 – 2289221DNA polymerase II large subunit, 3rd partSequence AnalysisPRO_0000294684Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit.UniRule annotation

Protein-protein interaction databases

STRINGi362976.HQ3461A.

Structurei

3D structure databases

ProteinModelPortaliQ18ER3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1222 – 1367146DOD-type homing endonuclease 1Add
BLAST
Domaini1755 – 1911157DOD-type homing endonuclease 2Add
BLAST

Sequence similaritiesi

Belongs to the archaeal DNA polymerase II family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1933.
KOiK02322.
OMAiEYPWSAA.

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 2 hits.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 4 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18ER3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRDADKQYFE TLESHLEQAF ARARQAKGQG YDPKPEVEIP VARDMADRVE
60 70 80 90 100
NILAIPDVAE RIRELDDERS REEVALELVT DFVEGTVGDY DTREGKIEGA
110 120 130 140 150
VRTAVALLTE GVVAAPIEGI DRVEILSDDD GSEFVNVYYA GPIRSAGGTA
160 170 180 190 200
QALSVLVADY ARSLLDIDEY SARTDETERY AEEVSLYDRE TGLQYSPKDK
210 220 230 240 250
ETKFITKHMP IMLDGEATGN EEVSGYRDLE RVDTNAARGG MCLVLAEGIA
260 270 280 290 300
LKAPKIQRYT RQLAEVEWPW LQDLIDDTIG SDEHSNNSVK NGEADIVKTD
310 320 330 340 350
KDTNESETED GIDNDDYNDS GLEPANSPRA DATNKYLRDL IAGRPVFGHP
360 370 380 390 400
SAAGAFRLRY GRARNHGFAT AGVHPATMHI VDDFIATGTQ LKTERPGKAG
410 420 430 440 450
GVVPVDSIKG PTVRLANGDV RCINDPEEAE KLQNGVEKIL DLGEYLVNFG
460 470 480 490 500
EFIENNHPLA PAAYVFEWWI QEFEASNADV QALRDDPTVD LESPTFENAM
510 520 530 540 550
RWAKKHDIPL HPAYTYLWHD ISVTEFDHLA DAVAAGEITM NEVSDTNSAS
560 570 580 590 600
GNTSLRANTN TDDTLTIDTT PAIRETMERL LIEHHQDSDS IHIPAWRALA
610 620 630 640 650
LSLGIKIESD NDTGIGDRMW SLTDLSKHAR KQDDGKSAIA AVNEVAPFQV
660 670 680 690 700
RERAPTRIGS RMGRPEKSED RDLSPAVHTL FPIGEAGGNQ RNLSDAAQSF
710 720 730 740 750
GDNTERGQIS VQLGKRRCPY CETVGFELQC AECGRHTEPQ FVCRECESVL
760 770 780 790 800
SPDESGRVHC DRCERDVTSA EWQDIDLHQR YRDALDRVDE RESSFEILKG
810 820 830 840 850
VKGLTSSNKT PEPLEKGILR AKHGVSSFKD GTVRYDMTDL PVTAVRPKEL
860 870 880 890 900
DVTAAHFREL GYQTDINGNP LQFDDQLVEL KVQDIVLPDG AAEHMMKTAD
910 920 930 940 950
FVDDLLEQFY ELNQFYQIDE RDDLIGELVF GMAPHTSAAV VGRIVGFTSA
960 970 980 990 1000
AVGYAHPYFH AAKRRNCFHP ETKIWFRDTD NEWHHETIQT FVEDRLDDPE
1010 1020 1030 1040 1050
IDAVGTLVQE VDDNTDREIS VPSIDDNGNE RLQSVTAVSK HRAPNHLVQI
1060 1070 1080 1090 1100
ETISGREITI TPDHEMHIFE QGNLVSKQAS KITSGEYAVI PKRLQTISPS
1110 1120 1130 1140 1150
SHTPQHDLLR EFLTRDELTA DRLIIHTSDP VRLCNRVFPE EVTSCKDAVE
1160 1170 1180 1190 1200
IMQNTACHLD KNKETLIGWL GEGRIPVALL RGFVETDEAL LMSIPDDVQI
1210 1220 1230 1240 1250
GLRGEKVRID RHIGFTEELT SLLGYYAAAG IVHTQTNPIS YESAQQEQSR
1260 1270 1280 1290 1300
ITFYNIDTQT QTDLLNALNS VFEIEPIQYN LDGEILGVPG ELIRRVFDTV
1310 1320 1330 1340 1350
FDVGTQPSHK RIPQALFDAS ESHITSYLRC FFSTHDSLTT DTRDISATTV
1360 1370 1380 1390 1400
SREFKEDIIA ALRRLGITAE VTTQQSRSVP EVLPDWYAID DITHHDADNS
1410 1420 1430 1440 1450
LNLTRSYVIS IASSDAVTLQ RDRQAQEQIK YDAQGLIANN NAIHQSRQVT
1460 1470 1480 1490 1500
DGGRKDYITE PITDIEYVDA DIDYTYCLTV SETHSLIVND LSQKQCDGDE
1510 1520 1530 1540 1550
DCVMLLMDGL LNFSREYLPD KRGGSVAADS RLVAVSPDDK IVFTTIEDFW
1560 1570 1580 1590 1600
KKLNTPIERN GKFRKRTCVS EGWQTYAFDE NHEASLRPIE KAIRYTADES
1610 1620 1630 1640 1650
EQLRRITTQL GRSLDITDEH SLFRYDDGIE EVAGDDLTAG DIIVAPRTLD
1660 1670 1680 1690 1700
VEVTQTTLDL SEYIHDNERC PSEQTGSGEL NLASKSAISD SRNKETPGVT
1710 1720 1730 1740 1750
HNILPQRSKF TDEMTTLSPT AVGGLESEQN ETLRVGESTG AIERYINVDD
1760 1770 1780 1790 1800
SFGWLLGQFI AQRSISTDAL TMTVHTAAEK HAERIVATSD SVFGIKPTVN
1810 1820 1830 1840 1850
SIERGYEIVF PSVFDTIVSG LTAKEQSEPE QDVDHTHTDE IGIPECILHA
1860 1870 1880 1890 1900
PDDIVLSFLQ GFILAENAQR KGNAASEASE MVSESETTVT LETPSVGVKD
1910 1920 1930 1940 1950
GLVFLCHRLG VITDISEKSG EEYSVHFEES RYTVSIATEG KTNPLDQILN
1960 1970 1980 1990 2000
GERPTMPEGV SVPVPDALLT IHESIANSPH IDQVIPDTVV QQETVSLETL
2010 2020 2030 2040 2050
QSLLTGLSTV DLPAQLEAKR DELTLLTEGD LSYLRVESVE CVDYDGYLYD
2060 2070 2080 2090 2100
LQVGGEPVFT ANWLYAHNSM DAPLVMSSRI DPSEIDDEAH NMDIVRQYPR
2110 2120 2130 2140 2150
EFYEATRRME DPDEWEEEVT IAEEYLDTDN EYTGFNHTHD TTDIAAGPDL
2160 2170 2180 2190 2200
SAYKTLDSMM DKMDAQLELA RKLRAVDETD VAERVIEYHF LPDLIGNLRA
2210 2220 2230 2240 2250
FSRQQTRCLD CGESYRRMPL TGECRECGGR VNLTVHEGSV NKYMDTAIHI
2260 2270 2280
AEEFDCREYT KQRLEVLERS LESIFEDDTN KQSGIADFM
Length:2,289
Mass (Da):255,812
Last modified:July 25, 2006 - v1
Checksum:iA5E70294203AFF05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA. Translation: CAJ53557.1.
RefSeqiWP_011572651.1. NC_008212.1.
YP_659142.1. NC_008212.1.

Genome annotation databases

EnsemblBacteriaiCAJ53557; CAJ53557; HQ_3461A.
GeneIDi4194482.
KEGGihwa:HQ3461A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180088 Genomic DNA. Translation: CAJ53557.1 .
RefSeqi WP_011572651.1. NC_008212.1.
YP_659142.1. NC_008212.1.

3D structure databases

ProteinModelPortali Q18ER3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362976.HQ3461A.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ53557 ; CAJ53557 ; HQ_3461A .
GeneIDi 4194482.
KEGGi hwa:HQ3461A.

Phylogenomic databases

eggNOGi COG1933.
KOi K02322.
OMAi EYPWSAA.

Enzyme and pathway databases

BioCyci HWAL362976:GJSR-2597-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 2 hits.
HAMAPi MF_00324. DNApol_II_L_arch.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view ]
Pfami PF03833. PolC_DP2. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 4 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEi PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
    Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
    BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16790 / HBSQ001.

Entry informationi

Entry nameiDP2L_HALWD
AccessioniPrimary (citable) accession number: Q18ER3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 25, 2006
Last modified: October 1, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3