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Q18ER3

- DP2L_HALWD

UniProt

Q18ER3 - DP2L_HALWD

Protein

DNA polymerase II large subunit

Gene

polC

Organism
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
    Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. endonuclease activity Source: UniProtKB-KW
    4. exonuclease activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA catabolic process Source: InterPro
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Endonuclease, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Intron homing

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciHWAL362976:GJSR-2597-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
    Short name:
    Pol IIUniRule annotation
    Cleaved into the following 2 chains:
    Alternative name(s):
    Hwa pol II 1 intein
    Alternative name(s):
    Hwa pol II 2 intein
    Gene namesi
    Name:polCUniRule annotation
    Synonyms:polA2
    Ordered Locus Names:HQ_3461A
    OrganismiHaloquadratum walsbyi (strain DSM 16790 / HBSQ001)
    Taxonomic identifieri362976 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloquadratum
    ProteomesiUP000001975: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 966966DNA polymerase II large subunit, 1st partSequence AnalysisPRO_0000294680Add
    BLAST
    Chaini967 – 1495529Hwa polC 1 inteinSequence AnalysisPRO_0000294681Add
    BLAST
    Chaini1496 – 152429DNA polymerase II large subunit, 2nd partSequence AnalysisPRO_0000294682Add
    BLAST
    Chaini1525 – 2068544Hwa polC 2 inteinSequence AnalysisPRO_0000294683Add
    BLAST
    Chaini2069 – 2289221DNA polymerase II large subunit, 3rd partSequence AnalysisPRO_0000294684Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Heterodimer of a large subunit and a small subunit.UniRule annotation

    Protein-protein interaction databases

    STRINGi362976.HQ3461A.

    Structurei

    3D structure databases

    ProteinModelPortaliQ18ER3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1222 – 1367146DOD-type homing endonuclease 1Add
    BLAST
    Domaini1755 – 1911157DOD-type homing endonuclease 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the archaeal DNA polymerase II family.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1933.
    KOiK02322.
    OMAiEYPWSAA.

    Family and domain databases

    Gene3Di2.170.16.10. 4 hits.
    3.10.28.10. 2 hits.
    HAMAPiMF_00324. DNApol_II_L_arch.
    InterProiIPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR004475. PolC_DP2.
    IPR016033. PolC_DP2_N.
    [Graphical view]
    PfamiPF03833. PolC_DP2. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 4 hits.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
    TIGR00354. polC. 1 hit.
    PROSITEiPS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q18ER3-1 [UniParc]FASTAAdd to Basket

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    MRDADKQYFE TLESHLEQAF ARARQAKGQG YDPKPEVEIP VARDMADRVE     50
    NILAIPDVAE RIRELDDERS REEVALELVT DFVEGTVGDY DTREGKIEGA 100
    VRTAVALLTE GVVAAPIEGI DRVEILSDDD GSEFVNVYYA GPIRSAGGTA 150
    QALSVLVADY ARSLLDIDEY SARTDETERY AEEVSLYDRE TGLQYSPKDK 200
    ETKFITKHMP IMLDGEATGN EEVSGYRDLE RVDTNAARGG MCLVLAEGIA 250
    LKAPKIQRYT RQLAEVEWPW LQDLIDDTIG SDEHSNNSVK NGEADIVKTD 300
    KDTNESETED GIDNDDYNDS GLEPANSPRA DATNKYLRDL IAGRPVFGHP 350
    SAAGAFRLRY GRARNHGFAT AGVHPATMHI VDDFIATGTQ LKTERPGKAG 400
    GVVPVDSIKG PTVRLANGDV RCINDPEEAE KLQNGVEKIL DLGEYLVNFG 450
    EFIENNHPLA PAAYVFEWWI QEFEASNADV QALRDDPTVD LESPTFENAM 500
    RWAKKHDIPL HPAYTYLWHD ISVTEFDHLA DAVAAGEITM NEVSDTNSAS 550
    GNTSLRANTN TDDTLTIDTT PAIRETMERL LIEHHQDSDS IHIPAWRALA 600
    LSLGIKIESD NDTGIGDRMW SLTDLSKHAR KQDDGKSAIA AVNEVAPFQV 650
    RERAPTRIGS RMGRPEKSED RDLSPAVHTL FPIGEAGGNQ RNLSDAAQSF 700
    GDNTERGQIS VQLGKRRCPY CETVGFELQC AECGRHTEPQ FVCRECESVL 750
    SPDESGRVHC DRCERDVTSA EWQDIDLHQR YRDALDRVDE RESSFEILKG 800
    VKGLTSSNKT PEPLEKGILR AKHGVSSFKD GTVRYDMTDL PVTAVRPKEL 850
    DVTAAHFREL GYQTDINGNP LQFDDQLVEL KVQDIVLPDG AAEHMMKTAD 900
    FVDDLLEQFY ELNQFYQIDE RDDLIGELVF GMAPHTSAAV VGRIVGFTSA 950
    AVGYAHPYFH AAKRRNCFHP ETKIWFRDTD NEWHHETIQT FVEDRLDDPE 1000
    IDAVGTLVQE VDDNTDREIS VPSIDDNGNE RLQSVTAVSK HRAPNHLVQI 1050
    ETISGREITI TPDHEMHIFE QGNLVSKQAS KITSGEYAVI PKRLQTISPS 1100
    SHTPQHDLLR EFLTRDELTA DRLIIHTSDP VRLCNRVFPE EVTSCKDAVE 1150
    IMQNTACHLD KNKETLIGWL GEGRIPVALL RGFVETDEAL LMSIPDDVQI 1200
    GLRGEKVRID RHIGFTEELT SLLGYYAAAG IVHTQTNPIS YESAQQEQSR 1250
    ITFYNIDTQT QTDLLNALNS VFEIEPIQYN LDGEILGVPG ELIRRVFDTV 1300
    FDVGTQPSHK RIPQALFDAS ESHITSYLRC FFSTHDSLTT DTRDISATTV 1350
    SREFKEDIIA ALRRLGITAE VTTQQSRSVP EVLPDWYAID DITHHDADNS 1400
    LNLTRSYVIS IASSDAVTLQ RDRQAQEQIK YDAQGLIANN NAIHQSRQVT 1450
    DGGRKDYITE PITDIEYVDA DIDYTYCLTV SETHSLIVND LSQKQCDGDE 1500
    DCVMLLMDGL LNFSREYLPD KRGGSVAADS RLVAVSPDDK IVFTTIEDFW 1550
    KKLNTPIERN GKFRKRTCVS EGWQTYAFDE NHEASLRPIE KAIRYTADES 1600
    EQLRRITTQL GRSLDITDEH SLFRYDDGIE EVAGDDLTAG DIIVAPRTLD 1650
    VEVTQTTLDL SEYIHDNERC PSEQTGSGEL NLASKSAISD SRNKETPGVT 1700
    HNILPQRSKF TDEMTTLSPT AVGGLESEQN ETLRVGESTG AIERYINVDD 1750
    SFGWLLGQFI AQRSISTDAL TMTVHTAAEK HAERIVATSD SVFGIKPTVN 1800
    SIERGYEIVF PSVFDTIVSG LTAKEQSEPE QDVDHTHTDE IGIPECILHA 1850
    PDDIVLSFLQ GFILAENAQR KGNAASEASE MVSESETTVT LETPSVGVKD 1900
    GLVFLCHRLG VITDISEKSG EEYSVHFEES RYTVSIATEG KTNPLDQILN 1950
    GERPTMPEGV SVPVPDALLT IHESIANSPH IDQVIPDTVV QQETVSLETL 2000
    QSLLTGLSTV DLPAQLEAKR DELTLLTEGD LSYLRVESVE CVDYDGYLYD 2050
    LQVGGEPVFT ANWLYAHNSM DAPLVMSSRI DPSEIDDEAH NMDIVRQYPR 2100
    EFYEATRRME DPDEWEEEVT IAEEYLDTDN EYTGFNHTHD TTDIAAGPDL 2150
    SAYKTLDSMM DKMDAQLELA RKLRAVDETD VAERVIEYHF LPDLIGNLRA 2200
    FSRQQTRCLD CGESYRRMPL TGECRECGGR VNLTVHEGSV NKYMDTAIHI 2250
    AEEFDCREYT KQRLEVLERS LESIFEDDTN KQSGIADFM 2289
    Length:2,289
    Mass (Da):255,812
    Last modified:July 25, 2006 - v1
    Checksum:iA5E70294203AFF05
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180088 Genomic DNA. Translation: CAJ53557.1.
    RefSeqiWP_011572651.1. NC_008212.1.
    YP_659142.1. NC_008212.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ53557; CAJ53557; HQ_3461A.
    GeneIDi4194482.
    KEGGihwa:HQ3461A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180088 Genomic DNA. Translation: CAJ53557.1 .
    RefSeqi WP_011572651.1. NC_008212.1.
    YP_659142.1. NC_008212.1.

    3D structure databases

    ProteinModelPortali Q18ER3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 362976.HQ3461A.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ53557 ; CAJ53557 ; HQ_3461A .
    GeneIDi 4194482.
    KEGGi hwa:HQ3461A.

    Phylogenomic databases

    eggNOGi COG1933.
    KOi K02322.
    OMAi EYPWSAA.

    Enzyme and pathway databases

    BioCyci HWAL362976:GJSR-2597-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 4 hits.
    3.10.28.10. 2 hits.
    HAMAPi MF_00324. DNApol_II_L_arch.
    InterProi IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR004475. PolC_DP2.
    IPR016033. PolC_DP2_N.
    [Graphical view ]
    Pfami PF03833. PolC_DP2. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 4 hits.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
    TIGR00354. polC. 1 hit.
    PROSITEi PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity."
      Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D.
      BMC Genomics 7:169-169(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 16790 / HBSQ001.

    Entry informationi

    Entry nameiDP2L_HALWD
    AccessioniPrimary (citable) accession number: Q18ER3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3