Reviewed, UniProtKB/Swiss-Prot Q18E75 (RFCS_HALWD)

Names and origin

Protein names

Recommended name:
    Replication factor C small subunit
      Short name=RFC small subunit
Alternative name(s):
    Clamp loader small subunit
Cleaved into the following chain:
    1- Recommended name:
            Hwa RFC intein

Gene names

Name:	rfcS
Ordered Locus Names:	HQ3671A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA By similarity.
Subunit structure	Heteromultimer composed of small subunits (rfcS) and large subunits (rfcL) By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	DNA replication factor C complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA clamp loader activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Replication factor C small subunit, 1st part Potential		PRO_0000296647
	Chain	62 – 384	323	Hwa RFC intein Potential		PRO_0000296648
	Chain	385 – 649	265	Replication factor C small subunit, 2nd part Potential		PRO_0000296649
Regions
	Nucleotide binding	55 – 385	331	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q18E75-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 7FB7838FA03877CC	FASTA	649	70,442
	10 20 30 40 50 60 MSEADASDAA PTGREIWIEK YRPATLENIY GQEDTVDRLQ SYIDRDDLPH LLFAGPAGVG 70 80 90 100 110 120 KCVTGSTPIL TNKGIRQIGE IVGDVDGFAP APQNLKVCSL TADGSFQYRH PSHVFGKRAS 130 140 150 160 170 180 GLQRIKTNDG ATLTVTPEHK LLIRTGENTN PTWVPAADIT AGMHVLRAKN LPIPAETTGS 190 200 210 220 230 240 CAASKNASEV SHIGDEYRYH DSLMADVNTR IATLERLIED YAESRSDGSL KFTLIGAHTP 250 260 270 280 290 300 TVSTVSYLLA TVGIASRHTS TLIDSEKRVH AIIIDASDTV RLEEMIETDW DTVMADQTTT 310 320 330 340 350 360 VTSSSTASTT KTTQSYLSSG ETQTCGWIPY ADGGVTHPST QHSPLHADVV TVSESLDAEK 370 380 390 400 410 420 RVYDLTVPGV RNYVGGCIPT VMHNTTAATA IAHAVYGDDW QNNLLELNAS DERGIDVVRD 430 440 450 460 470 480 RIKNFARSSF GGYDHRIIFL DEADSLTDDA QSALRRTMEQ FADNTRFILS CNYSSKIIDP 490 500 510 520 530 540 IQSRCAVFRF SPLSETAIRG QTKDIAAAEN IELTEDGLDA LVYAAGGDMR RAINSLQAAA 550 560 570 580 590 600 TTGEVVDEET VYTITSTARP EDIETMVTAA IDGDFTTARS QLQTLLVDTG MAGGDIIDQL 610 620 630 640 HRTAWNLDLD EETTVRLLER VGEADYRITV GANEQVQLEA LLASLADTQ

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed: 16820047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM180088 Genomic DNA. Translation: CAJ53758.1.
RefSeq	YP_659332.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q18E75.
Genome annotation databases
GeneID	4194575.
GenomeReviews	Gene locus HQ3671A in contig AM180088_GR.
KEGG	hwa:HQ3671A.
NMPDR	fig|362976.6.peg.2654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q18E75.
OMA	NASEVSH
Enzyme and pathway databases
BioCyc	HWAL362976:HQ3671A-MON.
Family and domain databases
HAMAP	MF_01509. [Tree]
InterPro	IPR008921. DNA_pol3_clamp-load_cplx_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR006141. Intein_splicing_site. IPR013748. Replication_factor_C. [Graphical view]
Pfam	PF08542. Rep_fac_C. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_HALWD

Accession

Primary (citable) accession number: Q18E75

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 25, 2006
Last modified:	November 24, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)