Q18E75 (RFCS_HALWD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication factor C small subunit
Short name=RFC small subunit

Alternative name(s):
Clamp loader small subunit

Cleaved into the following chain:

1. Hwa RFC intein

Gene names

Name:	rfcS
Ordered Locus Names:	HQ3671A

Organism

Haloquadratum walsbyi (strain DSM 16790) [Complete proteome] [HAMAP]

Taxonomic identifier

362976 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloquadratum

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA By similarity. HAMAP MF_01509
Subunit structure	Heteromultimer composed of small subunits (rfcS) and large subunits (rfcL) By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_01509
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	DNA replication factor C complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA clamp loader activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Replication factor C small subunit, 1st part Potential		PRO_0000296647
	Chain	62 – 384	323	Hwa RFC intein Potential		PRO_0000296648
	Chain	385 – 649	265	Replication factor C small subunit, 2nd part Potential		PRO_0000296649
Regions
	Nucleotide binding	55 – 385	331	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q18E75 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 7FB7838FA03877CC	FASTA	649	70,442
	10 20 30 40 50 60 MSEADASDAA PTGREIWIEK YRPATLENIY GQEDTVDRLQ SYIDRDDLPH LLFAGPAGVG 70 80 90 100 110 120 KCVTGSTPIL TNKGIRQIGE IVGDVDGFAP APQNLKVCSL TADGSFQYRH PSHVFGKRAS 130 140 150 160 170 180 GLQRIKTNDG ATLTVTPEHK LLIRTGENTN PTWVPAADIT AGMHVLRAKN LPIPAETTGS 190 200 210 220 230 240 CAASKNASEV SHIGDEYRYH DSLMADVNTR IATLERLIED YAESRSDGSL KFTLIGAHTP 250 260 270 280 290 300 TVSTVSYLLA TVGIASRHTS TLIDSEKRVH AIIIDASDTV RLEEMIETDW DTVMADQTTT 310 320 330 340 350 360 VTSSSTASTT KTTQSYLSSG ETQTCGWIPY ADGGVTHPST QHSPLHADVV TVSESLDAEK 370 380 390 400 410 420 RVYDLTVPGV RNYVGGCIPT VMHNTTAATA IAHAVYGDDW QNNLLELNAS DERGIDVVRD 430 440 450 460 470 480 RIKNFARSSF GGYDHRIIFL DEADSLTDDA QSALRRTMEQ FADNTRFILS CNYSSKIIDP 490 500 510 520 530 540 IQSRCAVFRF SPLSETAIRG QTKDIAAAEN IELTEDGLDA LVYAAGGDMR RAINSLQAAA 550 560 570 580 590 600 TTGEVVDEET VYTITSTARP EDIETMVTAA IDGDFTTARS QLQTLLVDTG MAGGDIIDQL 610 620 630 640 HRTAWNLDLD EETTVRLLER VGEADYRITV GANEQVQLEA LLASLADTQ

References

[1]

"The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity." Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., Pfeiffer F., Oesterhelt D. BMC Genomics 7:169-169(2006) [PubMed: 16820047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16790.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180088 Genomic DNA. Translation: CAJ53758.1.
RefSeq	YP_659332.1. NC_008212.1.
3D structure databases
ProteinModelPortal	Q18E75.
SMR	Q18E75. Positions 17-61, 403-647.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18E75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4194575.
GenomeReviews	Gene locus HQ3671A in contig AM180088_GR.
KEGG	hwa:HQ3671A.
NMPDR	fig|362976.6.peg.2654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04037.
HOGENOM	HBG120083.
PhylomeDB	Q18E75.
ProtClustDB	CLSK519869.
Enzyme and pathway databases
BioCyc	HWAL362976:HQ3671A-MONOMER.
Family and domain databases
HAMAP	MF_01509. RfcS. [Tree]
InterPro	IPR003959. ATPase_AAA_core. IPR008921. DNA_pol3_clamp-load_cplx_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR006141. Intein_splice_site. IPR013748. Rep_factor-C. [Graphical view]
KO	K04801.
Pfam	PF00004. AAA. 1 hit. PF08542. Rep_fac_C. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMs	TIGR01445. Intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_HALWD

Accession

Primary (citable) accession number: Q18E75

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 25, 2006
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program