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Q18CW0 (PUR9_PEPD6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:CD630_02230
OrganismPeptoclostridium difficile (strain 630) (Clostridium difficile) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_1000018878

Sequences

Sequence LengthMass (Da)Tools
Q18CW0 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 44E6415D06DE2528

FASTA51057,107
        10         20         30         40         50         60 
MSKRALISVT DKTGVVEFAK ELNKLDYEII STGNTFKTLK ENGVNVMQVE DVTNFPEILD 

        70         80         90        100        110        120 
GRVKTLNPYI HGGILYKRDK ESHVETVNEH KIHSIDLVAV NLYDFEGTLK AGKSHDEIIE 

       130        140        150        160        170        180 
NIDIGGPSMI RSAAKNYKDV IVVVDIKDYD SIIEKLKTDT MTLEDRKKLS YKAFSTTGRY 

       190        200        210        220        230        240 
DALISSYFAG EVGDTYPDIL NLTFQKEQTL RYGENPHQNG FLYSQSNAKN PILNYEQLGG 

       250        260        270        280        290        300 
KELSFNNLND LHGCLEVMRE FKDSEEVVSV AIKHANSCGV GLGKDAFEAY TKCYEADKVS 

       310        320        330        340        350        360 
IFGGIVGITS TIDKATAEKL NEIFLEIVVA YDFEPEALEI LKQKKNLRIL KLAKIENSLQ 

       370        380        390        400        410        420 
PYEMKYLDGK LLIQDRNNIL AEKSENVTKE KPTDAQLKDM EFGMRVVKNM KSNAIAIVKN 

       430        440        450        460        470        480 
GQTLALGCGQ TSRIWALKNA LENNKDKDFT GAVLASDAFF PFDDCVTLAH EYGISAVVQP 

       490        500        510 
GGSIKDKDSI EACDKYDMVM VFTGIRHFKH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180355 Genomic DNA. Translation: CAJ67044.1.
RefSeqYP_001086691.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ18CW0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272563.CD0223.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ67044; CAJ67044; CD630_02230.
GeneID4916698.
KEGGcdf:CD630_02230.
PATRIC19438689. VBICloDif38397_0241.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMAHRRAHAC.
OrthoDBEOG6QCDFF.

Enzyme and pathway databases

BioCycCDIF272563:GJFE-335-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_PEPD6
AccessionPrimary (citable) accession number: Q18CW0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways