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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.UniRule annotation

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.UniRule annotation

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821NucleophileUniRule annotation
Active sitei169 – 1691UniRule annotation
Active sitei171 – 1711UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi223 – 2297ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-310-MONOMER.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing]UniRule annotation (EC:6.3.5.2UniRule annotation)
Alternative name(s):
GMP synthetaseUniRule annotation
Glutamine amidotransferaseUniRule annotation
Gene namesi
Name:guaAUniRule annotation
Ordered Locus Names:CD630_01980
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511GMP synthase [glutamine-hydrolyzing]PRO_1000120262Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0198.

Structurei

3D structure databases

ProteinModelPortaliQ18CT8.
SMRiQ18CT8. Positions 1-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 195191Glutamine amidotransferase type-1UniRule annotationAdd
BLAST
Domaini196 – 386191GMPS ATP-PPaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiMSHGDSV.
OrthoDBiEOG6JHRJV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18CT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHELVLVID FGGQYNQLIA RRVRENNVYC EILPCTASIE RIKEKNPKGI
60 70 80 90 100
IFTGGPNSAY LEDSPTISKE IFELGVPILG ICYGIQIMSH VLGGVVRKGN
110 120 130 140 150
KQEKEYGKTA ITYGKSSLFE GITTNSVWMS HTDLIEKVPE GFTIVANTND
160 170 180 190 200
CPVAAMENVE RNLYGVQFHP EVEHCLEGDK ILTNFLYNIC KVKGDWTTDS
210 220 230 240 250
FIEDKIKELK EKIGDKKALC ALSGGVDSSV AAVLIHKAIG DNLTCIFVDH
260 270 280 290 300
GLLRKNEGND VERIFREKFD INLIRVNAED RFLSKLKGVS EPEAKRKIIG
310 320 330 340 350
EEFIRVFEEE SNKLGKMDFL VQGTIYPDVI ESGHGNAATI KSHHNVGGIP
360 370 380 390 400
EDVDFQEIVE PLRELFKDEV RKIGLELGIE EGLIFRHPFP GPGLGIRVIG
410 420 430 440 450
DVTKEKCDIL READAVYMDE LRKAGLYREI WQAFATLPDV KTVGVMGDER
460 470 480 490 500
TYAYLVGLRA VTSSDGMTSD WYKMPYDVLE RISNRIINEV DGVNRVVYDI
510
TSKPPGTIEW E
Length:511
Mass (Da):57,325
Last modified:July 25, 2006 - v1
Checksum:i3DAEAD392B4E27D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67019.1.
RefSeqiWP_003425341.1. NZ_CP010905.1.
YP_001086666.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67019; CAJ67019; CD630_01980.
GeneIDi4915467.
KEGGicdf:CD630_01980.
pdc:CDIF630_00320.
PATRICi19438639. VBICloDif38397_0216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67019.1.
RefSeqiWP_003425341.1. NZ_CP010905.1.
YP_001086666.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18CT8.
SMRiQ18CT8. Positions 1-511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0198.

Protein family/group databases

MEROPSiC26.957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67019; CAJ67019; CD630_01980.
GeneIDi4915467.
KEGGicdf:CD630_01980.
pdc:CDIF630_00320.
PATRICi19438639. VBICloDif38397_0216.

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiMSHGDSV.
OrthoDBiEOG6JHRJV.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BioCyciCDIF272563:GJFE-310-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630.

Entry informationi

Entry nameiGUAA_PEPD6
AccessioniPrimary (citable) accession number: Q18CT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 25, 2006
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.