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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi114IronUniRule annotation1
Metal bindingi118IronUniRule annotation1
Binding sitei170SubstrateUniRule annotation1
Binding sitei183Substrate; via amide nitrogenUniRule annotation1
Binding sitei187SubstrateUniRule annotation1
Binding sitei277SubstrateUniRule annotation1
Metal bindingi305IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-256-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:CD630_01520
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003033281 – 338tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST338

Interactioni

Protein-protein interaction databases

STRINGi272563.CD0152.

Structurei

3D structure databases

ProteinModelPortaliQ18CP0.
SMRiQ18CP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 141Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiHLEGHIY.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiView protein in InterPro
IPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
PfamiView protein in Pfam
PF00814. Peptidase_M22. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiView protein in PROSITE
PS01016. GLYCOPROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q18CP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIITLAIE SSCDETAASV LKNGREVLSN IISTQIETHK KFGGVVPEVA
60 70 80 90 100
SRKHVENIDI VVQEALDKAN IGFNDIDHIA VTYGPGLVGA LLVGLSYAKA
110 120 130 140 150
LAYTLNIPLV GVNHIEGHLS ANYIEHKDLK PPFITLIVSG GHTHLVEVKD
160 170 180 190 200
YGKYEILGKT RDDASGEAFD KISRAMNLGY PGGPIIDNLA KNGNKHAIEF
210 220 230 240 250
PRAYLEEDSY DFSFSGLKSS VLNYLNGKRM KNEEIVVEDV AASFQEAVVE
260 270 280 290 300
VLSTKALKAV KDKGYNIITL SGGVASNSGL RAKITELAKD NGITVKYPPL
310 320 330
ILCTDNAAMI GCAGYYNFIN GKTHDMSLNA VPNLKINQ
Length:338
Mass (Da):36,493
Last modified:July 25, 2006 - v1
Checksum:iC34929210054FBA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ66972.1.
RefSeqiWP_011860650.1. NC_009089.1.
YP_001086621.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ66972; CAJ66972; CD630_01520.
GeneIDi31351692.
4914358.
KEGGicdf:CD630_01520.
pdc:CDIF630_00271.
PATRICifig|272563.120.peg.165.

Similar proteinsi

Entry informationi

Entry nameiTSAD_PEPD6
AccessioniPrimary (citable) accession number: Q18CP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 25, 2006
Last modified: June 7, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families