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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:CD630_00980
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2363852.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315DNA-directed RNA polymerase subunit alphaPRO_0000264492Add
BLAST

Proteomic databases

PRIDEiQ18CI5.

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0098.

Structurei

3D structure databases

ProteinModelPortaliQ18CI5.
SMRiQ18CI5. Positions 245-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 228228Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd
BLAST
Regioni245 – 31571Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd
BLAST

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTF. Bacteria.
COG0202. LUCA.
HOGENOMiHOG000218480.
KOiK03040.
OMAiLMKFRNF.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA. 1 hit.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q18CI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIEKPKVD IVELSEDYRY GKFVIEPLER GYGITIGNAL RRILLSSLPG
60 70 80 90 100
VAVNAIKIDG VLHEFSTIPG VKEDVTEIIL TLKELSATID GEGSRTLKIE
110 120 130 140 150
AQGPCSITGA DIICPPDVEI LSKDLAIATL DDNAKLNMEI FVDKGRGYVS
160 170 180 190 200
AEENKTENVP IGVLPVDSIY TPVEKVSYHV ENTRVGQKTD YDKLVLEVWT
210 220 230 240 250
NGSINPQEGI SLAAKVLVEH LNLFIDLTEH VSSVEIMVEK EEDQKEKVLE
260 270 280 290 300
MTIEELDLSV RSYNCLKRAG INTVEELANK SEDDMMKVRN LGKKSLEEVI
310
QKLEELGLGL KPSEE
Length:315
Mass (Da):34,920
Last modified:July 25, 2006 - v1
Checksum:iE75526C3C02D9B16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ66917.1.
RefSeqiWP_003427733.1. NZ_CP010905.1.
YP_001086566.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ66917; CAJ66917; CD630_00980.
GeneIDi4913146.
KEGGicdf:CD630_00980.
pdc:CDIF630_00168.
PATRICi19438343. VBICloDif38397_0113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ66917.1.
RefSeqiWP_003427733.1. NZ_CP010905.1.
YP_001086566.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18CI5.
SMRiQ18CI5. Positions 245-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0098.

Chemistry

ChEMBLiCHEMBL2363852.

Proteomic databases

PRIDEiQ18CI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ66917; CAJ66917; CD630_00980.
GeneIDi4913146.
KEGGicdf:CD630_00980.
pdc:CDIF630_00168.
PATRICi19438343. VBICloDif38397_0113.

Phylogenomic databases

eggNOGiENOG4105CTF. Bacteria.
COG0202. LUCA.
HOGENOMiHOG000218480.
KOiK03040.
OMAiLMKFRNF.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-157-MONOMER.

Miscellaneous databases

PROiQ18CI5.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA. 1 hit.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPOA_PEPD6
AccessioniPrimary (citable) accession number: Q18CI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.