Q18CF4 (EFG_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	CD630_00700

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Elongation factor G HAMAP MF_00054_B		PRO_0000263441
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18CF4 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 38922784AB8980D1	FASTA	688	75,896
	10 20 30 40 50 60 MARKFPLERT RNIGIMAHID AGKTTTTERI LFYTGQTHKI GETHEGASQM DWMEQEKERG 70 80 90 100 110 120 ITITSAATTA SWKDHRINII DTPGHVDFTV EVERSLRVLD GSVAVFCAKG GVEPQSENVW 130 140 150 160 170 180 RQAETYGVPR IAFVNKMDIL GADFYNVVSM MKSRLNSNAV PMQLPIGKED SFIGIIDLLK 190 200 210 220 230 240 MDAVIYKDDL GVEMEETDIP EDMKELAAEW REKLVESVAE TDEELMMKYL EGEELTIDEL 250 260 270 280 290 300 KVAIRKATIA CEMNPVFCGT AYRNKGVQLV IDAVLDYLPA PTDIPAIKGI LADGEEAERH 310 320 330 340 350 360 SSDEEPFSAL AFKIMTDPFV GKLAFFRVYS GTLESGSYVL NATKNKRERI GRILQMHANT 370 380 390 400 410 420 REEITKVYAG DIAAAVGLKD TTTGDTLCDP ANPIILESME FPEPVISVAI EPSSKAAQEK 430 440 450 460 470 480 MGIALQKLAE EDPTFTVKTD QETGQTIISG MGELHLEIIV DRLLREFKVE AKVGAPQVAY 490 500 510 520 530 540 RETITQPVDV EYKYSKQSGG RGQYGHVKIR VAPQEPGEGY KFTNKTVGGS VPKEYVGPVD 550 560 570 580 590 600 MGIQGAMQSG IVAGYPVVDV AVELYDGSYH EVDSSEMAFK MAGSMAFKDA MKKGNAVLLE 610 620 630 640 650 660 PYFKVEVVTP EDYMGDVMGD LNSRRGLIQG MEARSGAQVI NAFVPLSEMF GYSTDLRSST 670 680 QGRATYTMIF DHYEQVPASV AKKIAEGK

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ66885.1.
RefSeq	YP_001086534.1. NC_009089.1.
3D structure databases
ProteinModelPortal	Q18CF4.
SMR	Q18CF4. Positions 4-684.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18CF4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4914220.
GenomeReviews	Gene locus CD0070 in contig AM180355_GR.
KEGG	cdf:CD0070.
NMPDR	fig|1496.1.peg.3123.
PATRIC	19438279. VBICloDif38397_0081.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_CLOD6

Accession

Primary (citable) accession number: Q18CF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program