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Protein

Elongation factor Tu

Gene

tuf1

more
Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi82 – 865GTPUniRule annotation
Nucleotide bindingi137 – 1404GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-112-MONOMER.
CDIF272563:GJFE-126-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tuf1UniRule annotation
Synonyms:tufA
Ordered Locus Names:CD630_00580
AND
Name:tuf1UniRule annotation
Synonyms:tufB
Ordered Locus Names:CD630_00710
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
ProteomesiUP000001978: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Elongation factor TuPRO_0000337361Add
BLAST

Proteomic databases

PRIDEiQ18CE4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0071.

Structurei

3D structure databases

ProteinModelPortaliQ18CE4.
SMRiQ18CE4. Positions 2-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 206197tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 268G1By similarity
Regioni61 – 655G2By similarity
Regioni82 – 854G3By similarity
Regioni137 – 1404G4By similarity
Regioni175 – 1773G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18CE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAKYERTK PHVNIGTIGH VDHGKTTLTA AITKTLYDRY QLGEAVDFAN
60 70 80 90 100
IDKAPEERER GITISTAHVE YETPNRHYAH VDCPGHADYV KNMITGAAQM
110 120 130 140 150
DGAILVCSAT DGPMPQTREH ILLSRQVGVP YIVVFLNKCD MVDDEELLEL
160 170 180 190 200
VEMEVRDLLT EYDFPGDDTP IVRGSALMAL EDPKSEWGDK IVELFEQIDE
210 220 230 240 250
YIPAPERDTD KPFLMPVEDV FSITGRGTVA TGRVERGVLK VQDEVELVGL
260 270 280 290 300
TEAPRKVVVT GVEMFRKLLD QAQAGDNIGA LLRGVQRNEI ERGQVLAKTG
310 320 330 340 350
SVKAHTKFTA EVYVLKKEEG GRHTPFFDGY RPQFYFRTTD VTGACKLPEG
360 370 380 390
IEMVMPGDNV TMEVDLINSI VVEEGLRFSI REGGRTVASG VVATIIE
Length:397
Mass (Da):44,026
Last modified:July 25, 2006 - v1
Checksum:iD2B6360068769C99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ66872.1.
AM180355 Genomic DNA. Translation: CAJ66886.1.
RefSeqiYP_001086521.1. NC_009089.1.
YP_001086535.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ66872; CAJ66872; CD630_00580.
CAJ66886; CAJ66886; CD630_00710.
GeneIDi4914221.
4916670.
KEGGicdf:CD630_00580.
cdf:CD630_00710.
PATRICi19438253. VBICloDif38397_0068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ66872.1.
AM180355 Genomic DNA. Translation: CAJ66886.1.
RefSeqiYP_001086521.1. NC_009089.1.
YP_001086535.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18CE4.
SMRiQ18CE4. Positions 2-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0071.

Proteomic databases

PRIDEiQ18CE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ66872; CAJ66872; CD630_00580.
CAJ66886; CAJ66886; CD630_00710.
GeneIDi4914221.
4916670.
KEGGicdf:CD630_00580.
cdf:CD630_00710.
PATRICi19438253. VBICloDif38397_0068.

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-112-MONOMER.
CDIF272563:GJFE-126-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630.

Entry informationi

Entry nameiEFTU_PEPD6
AccessioniPrimary (citable) accession number: Q18CE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.