Reviewed,
UniProtKB/Swiss-Prot Q18CD7 (SYP2_CLOD6)
Last modified
February 9, 2010.
Version 29.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Prolyl-tRNA synthetase 2 EC=6.1.1.15 Alternative name(s): Proline--tRNA ligase 2 Short name=ProRS 2 | ||||
| Gene names |
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| Organism | Clostridium difficile (strain 630) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 272563 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium |
Protein attributes
| Sequence length | 481 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571 |
| Catalytic activity | ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571 |
| Subunit structure | Homodimer By similarity. HAMAP MF_01571 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_01571. |
| Domain | Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571 |
| Sequence similarities | Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 481 | 481 | Prolyl-tRNA synthetase 2 HAMAP MF_01571 | PRO_0000288406 | |||
Sequences
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References
| [1] | "The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. Parkhill J.Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AM180355 Genomic DNA. Translation: CAJ66864.1. |
| RefSeq | YP_001086513.1. |
3D structure databases | |
| SMR | Q18CD7. Positions 10-481. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q18CD7. |
Genome annotation databases | |
| GeneID | 4916662. |
| GenomeReviews | Gene locus CD0050 in contig AM180355_GR. |
| KEGG | cdf:CD0050. |
| NMPDR | fig|1496.1.peg.99. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG0442. |
| HOGENOM | HBG334108. |
| OMA | LIMVHSD. |
Family and domain databases | |
| HAMAP | MF_01571. Pro_tRNA_synth_type3. [Tree] |
| InterPro | IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view] |
| Gene3D | G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit. |
| PANTHER | PTHR11451:SF6. ProS_fam_I. 1 hit. |
| Pfam | PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view] |
| PRINTS | PR01046. TRNASYNTHPRO. |
| TIGRFAMs | TIGR00408. proS_fam_I. 1 hit. |
| PROSITE | PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SYP2_CLOD6 | ||||||||
| Accession | Primary (citable) accession number: Q18CD7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


