Reviewed, UniProtKB/Swiss-Prot Q18CD7 (SYP2_CLOD6)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 2
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 2
      Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	CD0050

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Prolyl-tRNA synthetase 2 HAMAP MF_01571		PRO_0000288406

Sequences

	Sequence		Length	Mass (Da)
	Q18CD7-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: DB35FDC8BADC924A	FASTA	481	55,146
	10 20 30 40 50 60 MAKNEKQFVE EITKMEDDFP QWYTDVITKT DLVDYAPVKG FMVVKPYGYA LWEKMQEFMD 70 80 90 100 110 120 KKFKETGHKN CYFPLLIPES LLNKEAEHVE GFAPEVAWVT HGGNKKLEER LCVRPTSETI 130 140 150 160 170 180 ICTMYAKWLK SYRELPYLYN QWCSVVRWEK STRPFLRTSE FLWQEGHTLH ETAEEAQEET 190 200 210 220 230 240 IQQLEVYKAL CEELLAMPVV AGQKSESEKF AGGERTYTIE AMMHDGKALQ SGTSHFLGQH 250 260 270 280 290 300 FTKAFDITFA DREGNLANPY HTSWGASTRL IGGLIMTHSD NRGLVLPPRV APIQVVIVPI 310 320 330 340 350 360 AAKKGNVMET VDKIYADLKA KGVAVEVDDR DNYTTGWKFN EWEMKGVPVR VEIGPKDIEN 370 380 390 400 410 420 NQAMVFRRDT LEKDSMPLEG LADAICDLFD VIHNDMFEKA RKHREDNTSI VENMDEFRKA 430 440 450 460 470 480 LEEKPGFIKT MWCGDAECEA KIKEETGATI RCLPFEQENL GHKCVYCGKE ADSMVVMAKA Y

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ66864.1.
RefSeq	YP_001086513.1.
3D structure databases
SMR	Q18CD7. Positions 10-481.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18CD7.
Genome annotation databases
GeneID	4916662.
GenomeReviews	Gene locus CD0050 in contig AM180355_GR.
KEGG	cdf:CD0050.
NMPDR	fig|1496.1.peg.99.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG334108.
OMA	LIMVHSD.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_CLOD6

Accession

Primary (citable) accession number: Q18CD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 25, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)