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Q18CD7 (SYP2_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Proline--tRNA ligase 2
EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase 2
Short name=ProRS 2
Gene names
Name:proS2
Ordered Locus Names:CD630_00500
OrganismClostridium difficile (strain 630) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571

Subunit structure

Homodimer By similarity. HAMAP MF_01571

Subcellular location

Cytoplasm By similarity HAMAP MF_01571.

Domain

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

proline-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481Proline--tRNA ligase 2 HAMAP MF_01571
PRO_0000288406

Sequences

Sequence LengthMass (Da)Tools
Q18CD7 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: DB35FDC8BADC924A

FASTA48155,146
        10         20         30         40         50         60 
MAKNEKQFVE EITKMEDDFP QWYTDVITKT DLVDYAPVKG FMVVKPYGYA LWEKMQEFMD 

        70         80         90        100        110        120 
KKFKETGHKN CYFPLLIPES LLNKEAEHVE GFAPEVAWVT HGGNKKLEER LCVRPTSETI 

       130        140        150        160        170        180 
ICTMYAKWLK SYRELPYLYN QWCSVVRWEK STRPFLRTSE FLWQEGHTLH ETAEEAQEET 

       190        200        210        220        230        240 
IQQLEVYKAL CEELLAMPVV AGQKSESEKF AGGERTYTIE AMMHDGKALQ SGTSHFLGQH 

       250        260        270        280        290        300 
FTKAFDITFA DREGNLANPY HTSWGASTRL IGGLIMTHSD NRGLVLPPRV APIQVVIVPI 

       310        320        330        340        350        360 
AAKKGNVMET VDKIYADLKA KGVAVEVDDR DNYTTGWKFN EWEMKGVPVR VEIGPKDIEN 

       370        380        390        400        410        420 
NQAMVFRRDT LEKDSMPLEG LADAICDLFD VIHNDMFEKA RKHREDNTSI VENMDEFRKA 

       430        440        450        460        470        480 
LEEKPGFIKT MWCGDAECEA KIKEETGATI RCLPFEQENL GHKCVYCGKE ADSMVVMAKA 


Y

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM180355 Genomic DNA. Translation: CAJ66864.1.
RefSeqYP_001086513.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ18CD7.
SMRQ18CD7. Positions 10-481.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ18CD7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4916662.
GenomeReviewsGene locus CD0050 in contig AM180355_GR.
KEGGcdf:CD0050.
NMPDRfig|1496.1.peg.99.
PATRIC19438235. VBICloDif38397_0059.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHBG334108.
OMAKFAEYEL.
ProtClustDBPRK08661.

Family and domain databases

HAMAPMF_01571. Pro_tRNA_synth_type3.
[Tree]
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-synth_IIa.
IPR004499. Pro-tRNA-synth_IIa_arc-type.
IPR017449. Pro-tRNA_synth_II.
IPR016061. Pro-tRNA_synth_II_C.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KOK01881.
PANTHERPTHR11451:SF6. ProS_fam_I. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SMARTSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMSSF52954. Anticodon_bd. 1 hit.
SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMsTIGR00408. ProS_fam_I. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP2_CLOD6
AccessionPrimary (citable) accession number: Q18CD7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 25, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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