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Q18CD6 (SYE_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:CD630_00510
OrganismClostridium difficile (strain 630) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceae

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_1000001890

Regions

Motif9 – 1911"HIGH" region HAMAP-Rule MF_00022
Motif258 – 2625"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2611ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q18CD6 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 6676EAF97EB1B6CB

FASTA49356,612
        10         20         30         40         50         60 
MNVRVRFAPS PTGFVHIGSL RTALYNYLFA KKMGGEYILR VEDTDQSRLV EGAIENMLNA 

        70         80         90        100        110        120 
MKWAGVNHSE GVILDDSGKV VQKGEYGPYI QSQRLDIYQE YIKQLLDSGK AYYCFCTKER 

       130        140        150        160        170        180 
LDEVRDAQRA AGETAKYDGH CKNLSKEEVE ANIKAGIPYV IRLRLPENHT IKFTDLVRGD 

       190        200        210        220        230        240 
MEFNTNDLDD QVLMKTDGFP TYHFAVVVDD YLMKITHVIR GEEWVSSTPK HVYLYEAFGW 

       250        260        270        280        290        300 
EAPVFVHLPN ILNKEKKKLS KRQGDVAVED FKKKGYLPEG LVNYVALVGW SPEDNQELFT 

       310        320        330        340        350        360 
MEELEKAFSV ERVSKSGGVF DTEKLNWVNQ HYIKDGDDAY LTDLAIPFLI EDGFITEEEA 

       370        380        390        400        410        420 
TNKYDFLKSM ISVLKEKLQY VKEVTEHASI FFGDKIEVET EEGREFLRLE HIPTLIDALR 

       430        440        450        460        470        480 
EKIEKTEVLN AEFVQAMLKE IQKEYKIKGK NLFMGSRIIL TGQMHGPDLP KVMEVLGKET 

       490 
CLNRIAYVKN NIL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180355 Genomic DNA. Translation: CAJ66865.1.
RefSeqYP_001086514.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ18CD6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272563.CD0051.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ66865; CAJ66865; CD630_00510.
GeneID4916663.
KEGGcdf:CD630_00510.
PATRIC19438237. VBICloDif38397_0060.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK09698.
OMALMLFGRD.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycCDIF272563:GJFE-105-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_CLOD6
AccessionPrimary (citable) accession number: Q18CD6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries