Reviewed, UniProtKB/Swiss-Prot Q18CD2 (SYP1_CLOD6)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 1
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 1
      Short name=ProRS 1

Gene names

Name:	proS1
Ordered Locus Names:	CD0049

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Prolyl-tRNA synthetase 1 HAMAP MF_01569		PRO_0000288320

Sequences

	Sequence		Length	Mass (Da)
	Q18CD2-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 2281503B4C0D44B8	FASTA	571	64,400
	10 20 30 40 50 60 MKMSKMFMPT LKEIPADAEI TSHQLMVRSG MIKKMTSGVY NQLPMGLRVF KKIEQIIREE 70 80 90 100 110 120 LNKKDCQEIL CAALLPSELW KESGRWTAMG EEMFRLKDRT EREYCLGPTH EEAFTDIIRQ 130 140 150 160 170 180 EITSYKQLPL NLYQIQVKYR DERRPRFGVM RTKTFTMKDA YSFDVDDKGL DKSYQDMFDA 190 200 210 220 230 240 YVSIFDRCGL ENSPVQADSG AIGGSTSAEF MVKSEVGEDE VVFCSGCDYA ANVERAESCN 250 260 270 280 290 300 LASQKEEMKE LEEVHTPGAA TIKELEEFLK TSPDKFAKTL VYEADGKTVV VVVRGDREVN 310 320 330 340 350 360 EIKVSNAIGS VIEFALATDD VVRKVTNAEV GFAGPIGINA DYVFIDKEIV EQRNIVVGAN 370 380 390 400 410 420 KTEYHIKNAN YGRDFEGIVG DFRNVQEGDK CIVCGKPLEI ARGVEVGHIF KLGTKYSESM 430 440 450 460 470 480 NANFIDKDGK SKPIVMGCYG IGVERTAAAI IEQHNDEKGI IWPLSVAPYH VVIVPANMKN 490 500 510 520 530 540 EEQISIAENI YNDLQAMGVE VLLDDRDERI GVKFNDSELI GIPMRITVGK NINEGKVEFK 550 560 570 LRHKEDKEII DIEEINEKVK AEFIRNNVRL G

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM180355 Genomic DNA. Translation: CAJ66863.1.
RefSeq	YP_001086512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q18CD2.
Genome annotation databases
GeneID	4916638.
GenomeReviews	Gene locus CD0049 in contig AM180355_GR.
KEGG	cdf:CD0049.
NMPDR	fig|1496.1.peg.100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q18CD2.
OMA	VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP1_CLOD6

Accession

Primary (citable) accession number: Q18CD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)