Reviewed, UniProtKB/Swiss-Prot Q18C47 (PYRC_CLOD6)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	CD1526

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Dihydroorotase		PRO_1000024080
Sites
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	62	1	Zinc 1 By similarity
	Metal binding	141	1	Zinc 1; via carbamate group By similarity
	Metal binding	141	1	Zinc 2; via carbamate group By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	231	1	Zinc 2 By similarity
	Metal binding	304	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	141	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18C47-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 1B97667E07506FDB	FASTA	421	45,976
	10 20 30 40 50 60 MILIKNGRVI DPKSQRDENI DLIIKENKIY KIGKFDESDE YEKIIDASGN VVAPGLVDVH 70 80 90 100 110 120 VHFRDPGFTY KEDIESGAKS AARGGFTTVI CMANTNPIVD NEDTFNYVKE KSKNACINVL 130 140 150 160 170 180 QAAAITKGFE GKELVDMEAL KKAGVPGFTD DGLPLMDSNL IMEAMIKAKE LDVPLSFHEE 190 200 210 220 230 240 DPSLVGNPGV NAGKVASELG LKGASSVAED VMVARDCMLA LKTGAKVDIQ HISSGVSVDM 250 260 270 280 290 300 VRFAKYLGAT VVAEASPHHF TLTEEDVLEF GTNAKMNPPL RSKWDRDKII EGLNDGTIEI 310 320 330 340 350 360 IATDHAPHSK EEKDREFIKA PSGIIGLETS LALGITNLVH KNHLSMMQLI EKMSINPAKL 370 380 390 400 410 420 YNLNIGFIEE GAVADIVIFN PEEEWTVESF VSKADNSPFK GKSLYGKVNY TICNGEIVYN A

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM180355 Genomic DNA. Translation: CAJ68391.1.
RefSeq	YP_001088027.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4914653.
GenomeReviews	Gene locus CD1526 in contig AM180355_GR.
KEGG	cdf:CD1526.
NMPDR	fig|1496.1.peg.3393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q18C47.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_CLOD6

Accession

Primary (citable) accession number: Q18C47

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	September 2, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)