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Q18BI4

- PNP_PEPD6

UniProt

Q18BI4 - PNP_PEPD6

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi488 – 4881MagnesiumUniRule annotation
    Metal bindingi494 – 4941MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCDIF272563:GJFE-1482-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:CD630_13180
    OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
    Taxonomic identifieri272563 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
    ProteomesiUP000001978: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 703703Polyribonucleotide nucleotidyltransferasePRO_0000329598Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272563.CD1318.

    Structurei

    3D structure databases

    ProteinModelPortaliQ18BI4.
    SMRiQ18BI4. Positions 620-692.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini555 – 61460KHUniRule annotationAdd
    BLAST
    Domaini624 – 69269S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q18BI4-1 [UniParc]FASTAAdd to Basket

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    MFEHKIFKMD FAGRELSVEI GKICEMASGS CIVRYSDSMV MVNTTKSAKP    50
    RDGIDFFPLS VDYEEKLYSV GKIPGGFLKR EGKPSEKAIL TSRLIDRPIR 100
    PLFPKGFRND VQVVATVLSV DQDCTPDIVA MIGSSIALSI SDIPFNGPTG 150
    SVCVGLVDGA FVVNPNAEQR EKSSMHLVVS GTKEAIMMVE AGADEVPDEV 200
    MLDAILFAHQ EIKKIVEFIE GIVAEVGKEK MPVELYHAGE EITQLVREFA 250
    TDKMKKAVQT FEKLERMENM DRVKEETLAH FEETLEDFED FVGDIEEVLQ 300
    DIIKEEVRKL IVHENVRPDN RKLEEIRPIW CETGMIPRAH GSAIFTRGQT 350
    QVLNVATLGA LGDVQKLDGL DEEENKRYMH HYNFPAYSVG EARPSRGPGR 400
    REIGHGALAE RALLPVIPSQ EEFPYAIRLV SEVLSSNGST SQASVCGSTL 450
    SLLDAGVPIK DMVAGIAMGL IKHDGKVAVL SDIQGMEDHL GDMDFKVAGT 500
    EYGITAIQMD IKIDGIDKEI LQRALKQAKE GRIHILGEMR KTISQPKPEL 550
    SPYAPKIVKM QINPDKIKDV IGPGGKIITK IIDETGVKID IEQTGEVFIS 600
    GIEIDMIKKA QELINNIVVE PEVGKTYKGK VSRIMNFGAF VEILPGKEGL 650
    LHISHIAHER VAKVEDVLNI GDEVEVKVTE IDEKGRVNLS RKVLLPKPEH 700
    KNK 703
    Length:703
    Mass (Da):77,834
    Last modified:July 25, 2006 - v1
    Checksum:i33A69E982C0E0A5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180355 Genomic DNA. Translation: CAJ68176.1.
    RefSeqiYP_001087814.1. NC_009089.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ68176; CAJ68176; CD630_13180.
    GeneIDi4914478.
    KEGGicdf:CD630_13180.
    PATRICi19440995. VBICloDif38397_1385.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180355 Genomic DNA. Translation: CAJ68176.1 .
    RefSeqi YP_001087814.1. NC_009089.1.

    3D structure databases

    ProteinModelPortali Q18BI4.
    SMRi Q18BI4. Positions 620-692.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272563.CD1318.

    Protocols and materials databases

    DNASUi 4914478.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ68176 ; CAJ68176 ; CD630_13180 .
    GeneIDi 4914478.
    KEGGi cdf:CD630_13180.
    PATRICi 19440995. VBICloDif38397_1385.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CDIF272563:GJFE-1482-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 630.

    Entry informationi

    Entry nameiPNP_PEPD6
    AccessioniPrimary (citable) accession number: Q18BI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3