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Protein

Translation initiation factor IF-2

Gene

infB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi155 – 162GTPUniRule annotation8
Nucleotide bindingi201 – 205GTPUniRule annotation5
Nucleotide bindingi255 – 258GTPUniRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1445-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2UniRule annotation
Gene namesi
Name:infBUniRule annotation
Ordered Locus Names:CD630_13090
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000082321 – 646Translation initiation factor IF-2Add BLAST646

Interactioni

Protein-protein interaction databases

STRINGi272563.CD1309.

Structurei

3D structure databases

ProteinModelPortaliQ18BH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 315tr-type GAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 162G1By similarity8
Regioni180 – 184G2By similarity5
Regioni201 – 204G3By similarity4
Regioni255 – 258G4By similarity4
Regioni291 – 293G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EEM. Bacteria.
COG0532. LUCA.
HOGENOMiHOG000076906.
KOiK02519.
OMAiETEITHD.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B. 1 hit.
InterProiIPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18BH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKTRVYQIA EELNISNEEL INKLAELDIN VTDKDSVLEG EELELALEML
60 70 80 90 100
GEDLSQENGN VIEIDGKLTV QVLATKLDKS PSEIIMKLMK MGTMATINQE
110 120 130 140 150
ISFEIAALAA KDYGFELTVA ESDDTEALEI EALMEIEEDK EEDLKPRPPV
160 170 180 190 200
VTVMGHVDHG KTSLLDAIRK TDVISGEAGG ITQHIGASEV KINGHKIVFL
210 220 230 240 250
DTPGHEAFTS MRARGAQVTD IAILVVAADD GIMPQTVEAI NHAKAAGVPL
260 270 280 290 300
IVAINKIDKP GANPDKVKQE LADQGLLVED WGGEVIAVPV SAKKKEGIDT
310 320 330 340 350
LLEMVLLVAE MEELRANPNK RAVGTVIEAE LDKGRGPVAT VLVQGGTLTV
360 370 380 390 400
GDPIVAGVAC GKVRAMINAK GKRVKTAGPS TAVEILGLSE VPQGGDQFVE
410 420 430 440 450
VPTDKIARSV AARRQQIVRD EMLKSTQRLS LDALFSQMSE GSIKDLNIVI
460 470 480 490 500
KADVQGSVQA VKQSLEKLSN EEVQVKVIHG GVGAVTESDI LLAAASNAII
510 520 530 540 550
IGFNVRPVPG AESLGEKENV DIRTYTIIYK AIEDIQAAMT GMLDPEYVDE
560 570 580 590 600
ETGKAEIREI YKISGVGTVA GCYVTNGKIF RNCKVRLVRD SIIIHEGELA
610 620 630 640
ALKRFKDDVK EVNSGYECGM SFVNYNDIKE GDIVEAYITK EVERKL
Length:646
Mass (Da):69,711
Last modified:July 25, 2006 - v1
Checksum:i7F74BD9FD52327D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68167.1.
RefSeqiWP_003438306.1. NZ_CP010905.1.
YP_001087805.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ68167; CAJ68167; CD630_13090.
GeneIDi4914468.
KEGGicdf:CD630_13090.
pdc:CDIF630_01465.
PATRICi19440977. VBICloDif38397_1376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68167.1.
RefSeqiWP_003438306.1. NZ_CP010905.1.
YP_001087805.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18BH4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68167; CAJ68167; CD630_13090.
GeneIDi4914468.
KEGGicdf:CD630_13090.
pdc:CDIF630_01465.
PATRICi19440977. VBICloDif38397_1376.

Phylogenomic databases

eggNOGiENOG4107EEM. Bacteria.
COG0532. LUCA.
HOGENOMiHOG000076906.
KOiK02519.
OMAiETEITHD.

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1445-MONOMER.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B. 1 hit.
InterProiIPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2_PEPD6
AccessioniPrimary (citable) accession number: Q18BH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.