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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei104NucleophileUniRule annotation1
Binding sitei128ATP; via amide nitrogenUniRule annotation1
Active sitei202Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1415-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.13UniRule annotation)
Gene namesi
Name:mnmAUniRule annotation
Synonyms:trmU
Ordered Locus Names:CD630_12810
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000095181 – 361tRNA-specific 2-thiouridylase MnmAAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 202AlternateUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei129Interaction with tRNAUniRule annotation1
Sitei341Interaction with tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi272563.CD1281.

Structurei

3D structure databases

ProteinModelPortaliQ18BE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 154Interaction with tRNAUniRule annotation3
Regioni308 – 309Interaction with tRNAUniRule annotation2

Sequence similaritiesi

Belongs to the MnmA/TRMU family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218045.
KOiK00566.
OMAiCIACNRY.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q18BE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFMNKKVMIG MSGGVDSSVA AYLLKQQGYD VIGVTMKLWQ DDDVVEIEGG
60 70 80 90 100
CCSLSAVEDA RRVANKIGIP FYVLNFREVF KEKVIDYFID EYLEGKTPNP
110 120 130 140 150
CIACNKHIKF DDFYKKARQI GCDYVATGHY AKIEKDESTG RYLLKKSVTD
160 170 180 190 200
KKDQTYALYN LTQEQLEHTL LPIGDYEKDR VREIAKEMGM AVHNKPDSQE
210 220 230 240 250
ICFVKDNDYA NYVKKHSKKR IEEGFFVDTK GNILGKHKGI LYYTIGQRKG
260 270 280 290 300
LGITFGKPMF VIDINPINNT IVLGDNEDLF KKELIAKDVN FISIDTLEEP
310 320 330 340 350
LRVQAKIRYS AKPSPATIHR VGEDTIKIVF DEAQRAITKG QSVVMYDGDI
360
VVGGGIIEKS L
Length:361
Mass (Da):40,848
Last modified:July 25, 2006 - v1
Checksum:iCE676C209281729C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68137.1.
RefSeqiWP_011861167.1. NC_009089.1.
YP_001087775.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ68137; CAJ68137; CD630_12810.
GeneIDi4913512.
KEGGicdf:CD630_12810.
PATRICi19440917. VBICloDif38397_1346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68137.1.
RefSeqiWP_011861167.1. NC_009089.1.
YP_001087775.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18BE2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1281.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68137; CAJ68137; CD630_12810.
GeneIDi4913512.
KEGGicdf:CD630_12810.
PATRICi19440917. VBICloDif38397_1346.

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218045.
KOiK00566.
OMAiCIACNRY.

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1415-MONOMER.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMA_PEPD6
AccessioniPrimary (citable) accession number: Q18BE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.