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Protein

Ribonuclease HII

Gene

rnhB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mn2+UniRule annotation, Mg2+UniRule annotationNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Divalent metal cationUniRule annotation
Metal bindingi80 – 801Divalent metal cationUniRule annotation
Metal bindingi171 – 1711Divalent metal cationUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-1423-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HIIUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HIIUniRule annotation
Gene namesi
Name:rnhBUniRule annotation
Ordered Locus Names:CD630_12620
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Ribonuclease HIIPRO_0000334882Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272563.CD1262.

Structurei

3D structure databases

ProteinModelPortaliQ18BC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UH2. Bacteria.
COG0164. LUCA.
HOGENOMiHOG000100288.
KOiK03470.
OMAiGFASHKG.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00052_B. RNase_HII_B. 1 hit.
InterProiIPR022898. RNase_HII.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Q18BC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKSVREIK EIIETLEVEK YMEYIELLRV DERKSVQGLA IKLAKKLDNI
60 70 80 90 100
RKEEERLETI NIFENEGYDK GYLYIGGIDE AGRGPLAGPV VASVVVFKKD
110 120 130 140 150
TKIEGVNDSK KLSEAKRDEL FEVIKEEALD YGIGIVNNEE IDEFNILNAT
160 170 180 190 200
YMAMKKAINC LKKAPDYLLV DAATIPGIDI SQNPIVKGDS KSISIAAASI
210 220 230 240 250
LAKVTRDSIM YQYDRVYPEY GFKSHKGYGT KEHYEAIEKY GITPIHRKSF

LKNIL
Length:255
Mass (Da):28,910
Last modified:July 25, 2006 - v1
Checksum:iA0493467ECBCE5C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68118.1.
RefSeqiWP_003438239.1. NZ_CP010905.1.
YP_001087756.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ68118; CAJ68118; CD630_12620.
GeneIDi4913741.
KEGGicdf:CD630_12620.
pdc:CDIF630_01415.
PATRICi19440879. VBICloDif38397_1327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68118.1.
RefSeqiWP_003438239.1. NZ_CP010905.1.
YP_001087756.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18BC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68118; CAJ68118; CD630_12620.
GeneIDi4913741.
KEGGicdf:CD630_12620.
pdc:CDIF630_01415.
PATRICi19440879. VBICloDif38397_1327.

Phylogenomic databases

eggNOGiENOG4108UH2. Bacteria.
COG0164. LUCA.
HOGENOMiHOG000100288.
KOiK03470.
OMAiGFASHKG.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-1423-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00052_B. RNase_HII_B. 1 hit.
InterProiIPR022898. RNase_HII.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNH2_PEPD6
AccessioniPrimary (citable) accession number: Q18BC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.