Reviewed, UniProtKB/Swiss-Prot Q18AR0 (THLA_CLOD6)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA acetyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Name:	thlA
Ordered Locus Names:	CD1059

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Acetyl-CoA acetyltransferase		PRO_5000077784
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	348	1	Proton acceptor By similarity
	Active site	378	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18AR0-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 7B6713CCC8F70899	FASTA	391	40,860
	10 20 30 40 50 60 MREVVIASAA RTAVGSFGGA FKSVSAVELG VTAAKEAIKR ANITPDMIDE SLLGGVLTAG 70 80 90 100 110 120 LGQNIARQIA LGAGIPVEKP AMTINIVCGS GLRSVSMASQ LIALGDADIM LVGGAENMSM 130 140 150 160 170 180 SPYLVPSARY GARMGDAAFV DSMIKDGLSD IFNNYHMGIT AENIAEQWNI TREEQDELAL 190 200 210 220 230 240 ASQNKAEKAQ AEGKFDEEIV PVVIKGRKGD TVVDKDEYIK PGTTMEKLAK LRPAFKKDGT 250 260 270 280 290 300 VTAGNASGIN DGAAMLVVMA KEKAEELGIE PLATIVSYGT AGVDPKIMGY GPVPATKKAL 310 320 330 340 350 360 EAANMTIEDI DLVEANEAFA AQSVAVIRDL NIDMNKVNVN GGAIAIGHPI GCSGARILTT 370 380 390 LLYEMKRRDA KTGLATLCIG GGMGTTLIVK R

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ67900.1.
RefSeq	YP_001087540.1.
3D structure databases
SMR	Q18AR0. Positions 4-390.
ModBase	Search...
Protein-protein interaction databases
STRING	Q18AR0.
Genome annotation databases
GeneID	4915191.
GenomeReviews	Gene locus CD1059 in contig AM180355_GR.
KEGG	cdf:CD1059.
NMPDR	fig|1496.1.peg.3819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HBG370930.
OMA	AMTINIV.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THLA_CLOD6

Accession

Primary (citable) accession number: Q18AR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 25, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)