Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucosamine-6-phosphate deaminase

Gene

nagB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.UniRule annotation

Catalytic activityi

Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3.UniRule annotation

Pathwayi: N-acetylneuraminate degradation

This protein is involved in step 5 of the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. N-acetylneuraminate lyase (nanA)
  2. no protein annotated in this organism
  3. Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE)
  4. no protein annotated in this organism
  5. Glucosamine-6-phosphate deaminase (nagB)
This subpathway is part of the pathway N-acetylneuraminate degradation, which is itself part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate, the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Proton acceptor; for enolization stepUniRule annotation1
Active sitei136For ring-opening stepUniRule annotation1
Active sitei138Proton acceptor; for ring-opening stepUniRule annotation1
Active sitei143For ring-opening stepUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1126-MONOMER.
UniPathwayiUPA00629; UER00684.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine-6-phosphate deaminaseUniRule annotation (EC:3.5.99.6UniRule annotation)
Alternative name(s):
GlcN6P deaminaseUniRule annotation
Short name:
GNPDAUniRule annotation
Glucosamine-6-phosphate isomeraseUniRule annotation
Gene namesi
Name:nagBUniRule annotation
Ordered Locus Names:CD630_10110
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000669701 – 249Glucosamine-6-phosphate deaminaseAdd BLAST249

Interactioni

Protein-protein interaction databases

STRINGi272563.CD1011.

Structurei

3D structure databases

ProteinModelPortaliQ18AL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKA. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
KOiK02564.
OMAiHYFMQKH.

Family and domain databases

CDDicd01399. GlcN6P_deaminase. 1 hit.
HAMAPiMF_01241. GlcN6P_deamin. 1 hit.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q18AL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLVCKDYD GMSKKAAEMI AAQIVLKPNS ILGLATGSTP VGMYRDLVKK
60 70 80 90 100
YNDNIVDFSD VMSFNLDEYY KLPISNDQSY DYFMKENLFN HVNIKPENTH
110 120 130 140 150
LPNGMADDIE KECMNYEASI DAAGGIDVQV LGIGRNAHIG FNEPDTKFAK
160 170 180 190 200
RTHVVELTES TIEANARFFK SREDVPKKAV SMGIGSILKS KKILLLASGE
210 220 230 240
EKADAVYNTV YGDITPEVPG SILQLHKDTI VIVDEAAASK LNPKDYKLV
Length:249
Mass (Da):27,590
Last modified:July 25, 2006 - v1
Checksum:i380E10F5C9DDF884
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67852.1.
RefSeqiWP_003437039.1. NZ_CP010905.1.
YP_001087492.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67852; CAJ67852; CD630_10110.
GeneIDi4914486.
KEGGicdf:CD630_10110.
pdc:CDIF630_01147.
PATRICi19440339. VBICloDif38397_1062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67852.1.
RefSeqiWP_003437039.1. NZ_CP010905.1.
YP_001087492.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18AL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67852; CAJ67852; CD630_10110.
GeneIDi4914486.
KEGGicdf:CD630_10110.
pdc:CDIF630_01147.
PATRICi19440339. VBICloDif38397_1062.

Phylogenomic databases

eggNOGiENOG4105CKA. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
KOiK02564.
OMAiHYFMQKH.

Enzyme and pathway databases

UniPathwayiUPA00629; UER00684.
BioCyciPDIF272563:G12WB-1126-MONOMER.

Family and domain databases

CDDicd01399. GlcN6P_deaminase. 1 hit.
HAMAPiMF_01241. GlcN6P_deamin. 1 hit.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNAGB_PEPD6
AccessioniPrimary (citable) accession number: Q18AL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.