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Protein

DNA polymerase IV

Gene

dinB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11MagnesiumUniRule annotation1
Sitei16Substrate discriminationUniRule annotation1
Metal bindingi106MagnesiumUniRule annotation1
Active sitei107UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1007-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:CD630_08990
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000848811 – 365DNA polymerase IVAdd BLAST365

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0899

Structurei

3D structure databases

ProteinModelPortaliQ18A91
SMRiQ18A91
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 188UmuCUniRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000082707
KOiK02346
OMAiGMQIREI

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q18A91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHYRKIIHI DMDAFYASIE QRDNKKLKGR PVIVGGNPQS RGVVATCSYE
60 70 80 90 100
ARKFGIHSAM PSAVAYNRCP YAVFVRPRFD VYKSVSEKIR DIFYRYTDLV
110 120 130 140 150
EPLSLDEAYL DVTKNKKNID SSIEIAKQIK KDIFREVGLT SSAGVSYNKF
160 170 180 190 200
LAKIASDLRK PNGLTVITEE NAQDFLDKLP VNKFFGVGKV TSNTLKNLGI
210 220 230 240 250
KTGYDLRCLN LFELENIFKK RGYELYKFAR GIDDRPVEPN RVRKSVGAET
260 270 280 290 300
TLSHNLDIDE EETRNILDEL CEEVCHRLKN SEKFGKTLTL KIKYEDFTKI
310 320 330 340 350
TRSLSLEHYI DEYNDIRSGV DNLLRNVEVN GKQIRLLGVT ISNLSDKKET
360
YKDITLFEYM DSIQM
Length:365
Mass (Da):42,009
Last modified:July 25, 2006 - v1
Checksum:i4730134CCFA5AF34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ67732.1
RefSeqiWP_011860981.1, NZ_CP010905.2
YP_001087373.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ67732; CAJ67732; CD630_08990
GeneIDi31352457
4914151
KEGGicdf:CD630_08990
pdc:CDIF630_01019
PATRICifig|272563.120.peg.923

Similar proteinsi

Entry informationi

Entry nameiDPO4_PEPD6
AccessioniPrimary (citable) accession number: Q18A91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health