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Protein

DNA polymerase IV

Gene

dinB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111MagnesiumUniRule annotation
Sitei16 – 161Substrate discriminationUniRule annotation
Metal bindingi106 – 1061MagnesiumUniRule annotation
Active sitei107 – 1071UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-1038-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:CD630_08990
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365DNA polymerase IVPRO_1000084881Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0899.

Structurei

3D structure databases

ProteinModelPortaliQ18A91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 188182UmuCUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiMQIREIF.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18A91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHYRKIIHI DMDAFYASIE QRDNKKLKGR PVIVGGNPQS RGVVATCSYE
60 70 80 90 100
ARKFGIHSAM PSAVAYNRCP YAVFVRPRFD VYKSVSEKIR DIFYRYTDLV
110 120 130 140 150
EPLSLDEAYL DVTKNKKNID SSIEIAKQIK KDIFREVGLT SSAGVSYNKF
160 170 180 190 200
LAKIASDLRK PNGLTVITEE NAQDFLDKLP VNKFFGVGKV TSNTLKNLGI
210 220 230 240 250
KTGYDLRCLN LFELENIFKK RGYELYKFAR GIDDRPVEPN RVRKSVGAET
260 270 280 290 300
TLSHNLDIDE EETRNILDEL CEEVCHRLKN SEKFGKTLTL KIKYEDFTKI
310 320 330 340 350
TRSLSLEHYI DEYNDIRSGV DNLLRNVEVN GKQIRLLGVT ISNLSDKKET
360
YKDITLFEYM DSIQM
Length:365
Mass (Da):42,009
Last modified:July 25, 2006 - v1
Checksum:i4730134CCFA5AF34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67732.1.
RefSeqiWP_011860981.1. NZ_CP010905.1.
YP_001087373.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67732; CAJ67732; CD630_08990.
GeneIDi4914151.
KEGGicdf:CD630_08990.
pdc:CDIF630_01019.
PATRICi19440091. VBICloDif38397_0938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67732.1.
RefSeqiWP_011860981.1. NZ_CP010905.1.
YP_001087373.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18A91.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67732; CAJ67732; CD630_08990.
GeneIDi4914151.
KEGGicdf:CD630_08990.
pdc:CDIF630_01019.
PATRICi19440091. VBICloDif38397_0938.

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiMQIREIF.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-1038-MONOMER.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO4_PEPD6
AccessioniPrimary (citable) accession number: Q18A91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.