Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyamine aminopropyltransferase

Gene

speE

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.UniRule annotation

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.UniRule annotation

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Polyamine aminopropyltransferase (speE)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71PolyamineUniRule annotation
Binding sitei31 – 311S-adenosylmethioninamineUniRule annotation
Binding sitei62 – 621PolyamineUniRule annotation
Binding sitei86 – 861PolyamineUniRule annotation
Binding sitei106 – 1061S-adenosylmethioninamineUniRule annotation
Active sitei156 – 1561Proton acceptorUniRule annotation
Binding sitei163 – 1631S-adenosylmethioninamine; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-1029-MONOMER.
UniPathwayiUPA00248; UER00314.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine aminopropyltransferaseUniRule annotation
Alternative name(s):
Putrescine aminopropyltransferaseUniRule annotation
Short name:
PAPTUniRule annotation
Spermidine synthaseUniRule annotation (EC:2.5.1.16UniRule annotation)
Short name:
SPDSUniRule annotation
Short name:
SPDSYUniRule annotation
Gene namesi
Name:speEUniRule annotation
Ordered Locus Names:CD630_08900
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Polyamine aminopropyltransferasePRO_1000011994Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0890.

Structurei

3D structure databases

ProteinModelPortaliQ18A85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 238237PABSUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382S-adenosylmethioninamine bindingUniRule annotation
Regioni157 – 1593Polyamine bindingUniRule annotation

Sequence similaritiesi

Belongs to the spermidine/spermine synthase family.UniRule annotation
Contains 1 PABS (polyamine biosynthesis) domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCX. Bacteria.
COG0421. LUCA.
HOGENOMiHOG000256146.
KOiK00797.
OMAiLMMVTEK.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q18A85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELWYTEEWT ENVRFSIKVN KHLFEGKSQF QRIDVFDSDE FGKFLTIDGL
60 70 80 90 100
MMVTYKDEFI YHEMITHVPM ATNLNIKKVL VIGGGDGGTV RELSRYPQIE
110 120 130 140 150
KIDMVEIDKM VVDVSKEYMD ICSCKLDDKR VSLYFEDGVN FVKCAHDKSY
160 170 180 190 200
DLIIVDSTDP IGPGEGLFST DFYKDCYRIL TDDGILVNQS ESPYFDFNAK
210 220 230 240 250
EMKRANKKLK QIFPISEVYQ AHIPTYPSGH WLFGFASKKL NPVKNQDRNG
260 270 280
WEKLSLKTKY YNSDIHLGSF MLPQYVKEML DEE
Length:283
Mass (Da):32,992
Last modified:July 25, 2006 - v1
Checksum:iE33FE32272E16047
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67723.1.
RefSeqiWP_009888722.1. NZ_CP010905.1.
YP_001087364.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67723; CAJ67723; CD630_08900.
GeneIDi4914142.
KEGGicdf:CD630_08900.
pdc:CDIF630_01010.
PATRICi19440073. VBICloDif38397_0929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67723.1.
RefSeqiWP_009888722.1. NZ_CP010905.1.
YP_001087364.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ18A85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67723; CAJ67723; CD630_08900.
GeneIDi4914142.
KEGGicdf:CD630_08900.
pdc:CDIF630_01010.
PATRICi19440073. VBICloDif38397_0929.

Phylogenomic databases

eggNOGiENOG4105CCX. Bacteria.
COG0421. LUCA.
HOGENOMiHOG000256146.
KOiK00797.
OMAiLMMVTEK.

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.
BioCyciCDIF272563:GJFE-1029-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEE_PEPD6
AccessioniPrimary (citable) accession number: Q18A85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.