ID   FTHS_CLOD6              Reviewed;         558 AA.
AC   Q189R2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   27-MAR-2024, entry version 99.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=CD630_07180;
OS   Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=272563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=630;
RX   PubMed=16804543; DOI=10.1038/ng1830;
RA   Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA   Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA   Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA   Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA   Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA   Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA   Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT   "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT   mobile, mosaic genome.";
RL   Nat. Genet. 38:779-786(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AM180355; CAJ67552.1; -; Genomic_DNA.
DR   RefSeq; WP_009888533.1; NZ_JAUPES010000005.1.
DR   RefSeq; YP_001087195.1; NC_009089.1.
DR   AlphaFoldDB; Q189R2; -.
DR   SMR; Q189R2; -.
DR   STRING; 272563.CD630_07180; -.
DR   EnsemblBacteria; CAJ67552; CAJ67552; CD630_07180.
DR   GeneID; 66353223; -.
DR   KEGG; cdf:CD630_07180; -.
DR   KEGG; pdc:CDIF630_00834; -.
DR   PATRIC; fig|272563.120.peg.739; -.
DR   eggNOG; COG2759; Bacteria.
DR   OrthoDB; 9761733at2; -.
DR   PhylomeDB; Q189R2; -.
DR   BioCyc; PDIF272563:G12WB-829-MONOMER; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001978; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..558
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000300518"
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   558 AA;  59986 MW;  9BEE9D0D5B83FE61 CRC64;
     MGFKSDIEIA QEAKPQDIRE VAKKLGLGED DVELYGKYKA KVDYNLLKRE TGKKAKLILT
     TAINPTPAGE GKTTTTIGVA DAFAKLDKNV LVALREPSLG PVFGVKGGAA GGGYAQVVPM
     EDINLHFTGD FHAIGAANNL LAAMLDNHIH QGNALRIDPK KITWRRCVDM NDRQLRNIVD
     GMGKKGDGAV RQDGFDITVA SEIMAAFCLA SDISDLKERL GNIIVGYSYE GEPVTARQLK
     ANGAMAALLK DALKPNLVQT LEGTPSFVHG GPFANIAHGC NSVIATRMAM HFADYVITEA
     GFGADLGAEK FLDIKCRMAN LKPDAVIIVA TVRALKYNGG VAKADLNNEN LEALEKGLPN
     LLKHVENITQ VYGLPAVVAI NRFPLDTEAE LKLVEDKCKE LGVNVALSEV WAKGGEGGIA
     VAKEVLRLLD EEENNFRFCY EDDLSIKDKI NAIATKIYGA DGVDYTPEAD KEIANLEKLG
     FTKVPVCMAK TQYSLTDDQT KLGRPTGFRI TVRQATISAG AGFIVALTGE IMKMPGLPKV
     PAAEKIDVDE NGVIAGLF
//