Q189R2 (FTHS_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	CD630_07180

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Peptostreptococcaceae › Clostridium difficile

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000300518
Regions
	Nucleotide binding	66 – 73	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q189R2 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 9BEE9D0D5B83FE61	FASTA	558	59,986
	10 20 30 40 50 60 MGFKSDIEIA QEAKPQDIRE VAKKLGLGED DVELYGKYKA KVDYNLLKRE TGKKAKLILT 70 80 90 100 110 120 TAINPTPAGE GKTTTTIGVA DAFAKLDKNV LVALREPSLG PVFGVKGGAA GGGYAQVVPM 130 140 150 160 170 180 EDINLHFTGD FHAIGAANNL LAAMLDNHIH QGNALRIDPK KITWRRCVDM NDRQLRNIVD 190 200 210 220 230 240 GMGKKGDGAV RQDGFDITVA SEIMAAFCLA SDISDLKERL GNIIVGYSYE GEPVTARQLK 250 260 270 280 290 300 ANGAMAALLK DALKPNLVQT LEGTPSFVHG GPFANIAHGC NSVIATRMAM HFADYVITEA 310 320 330 340 350 360 GFGADLGAEK FLDIKCRMAN LKPDAVIIVA TVRALKYNGG VAKADLNNEN LEALEKGLPN 370 380 390 400 410 420 LLKHVENITQ VYGLPAVVAI NRFPLDTEAE LKLVEDKCKE LGVNVALSEV WAKGGEGGIA 430 440 450 460 470 480 VAKEVLRLLD EEENNFRFCY EDDLSIKDKI NAIATKIYGA DGVDYTPEAD KEIANLEKLG 490 500 510 520 530 540 FTKVPVCMAK TQYSLTDDQT KLGRPTGFRI TVRQATISAG AGFIVALTGE IMKMPGLPKV 550 PAAEKIDVDE NGVIAGLF

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ67552.1.
RefSeq	YP_001087195.1. NC_009089.1.
3D structure databases
ProteinModelPortal	Q189R2.
SMR	Q189R2. Positions 6-556.
ModBase	Search...
Protein-protein interaction databases
STRING	272563.CD0718.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ67552; CAJ67552; CD630_07180.
GeneID	4916827.
KEGG	cdf:CD630_07180.
PATRIC	19439723. VBICloDif38397_0755.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	IIIGYNY.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	CDIF272563:GJFE-855-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CLOD6

Accession

Primary (citable) accession number: Q189R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program