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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotationNote: Binds 2 magnesium ions per tetramer.SAAS annotation
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi254 – 2541MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunitUniRule annotationSAAS annotation (EC:6.1.1.20UniRule annotationSAAS annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunitUniRule annotation
Gene namesi
Name:pheSUniRule annotationImported
Ordered Locus Names:CD630_06990Imported
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)Imported
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
ProteomesiUP000001978: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi272563.CD0699.

Structurei

3D structure databases

ProteinModelPortaliQ189P4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0016.
HOGENOMiHOG000242675.
KOiK01889.
OMAiMGKELNS.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q189P4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEKLLALRE AALAEIKEAQ SIESVESLRV KYLGKKGEIT AILKEMGKLS
60 70 80 90 100
AEERPVVGKV ANEVRENIEL SINSKKEEIN AIEKERKLKE EVIDVTQPGK
110 120 130 140 150
VLKVGKKHPI TQIIDEVTDI FIGMGFSIAE GPEVETVENN FDALNAPKDH
160 170 180 190 200
PSRDMSDTFY INDGVLLRTQ TSPVQVRTMR SQELPIKVIA PGRCFRSDSP
210 220 230 240 250
DATHSPMFHQ IEGLVVGKDV TMAEFKGTMD IFVEKLFGSD IKTKFRPHNF
260 270 280 290 300
PFTEPSAEVD VTCFKCGGKG CPMCKYEGWI EILGSGMVHP NVLRNCGIDP
310 320 330
EVYSGFAFGV GVERLAMLKY EIDDIRLLFE NDMRFLNQF
Length:339
Mass (Da):38,004
Last modified:June 28, 2011 - v2
Checksum:i16B501BEA3EE3C32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67532.2.
RefSeqiYP_001087175.2. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67532; CAJ67532; CD630_06990.
GeneIDi4916807.
KEGGicdf:CD630_06990.
PATRICi19439681. VBICloDif38397_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67532.2.
RefSeqiYP_001087175.2. NC_009089.1.

3D structure databases

ProteinModelPortaliQ189P4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67532; CAJ67532; CD630_06990.
GeneIDi4916807.
KEGGicdf:CD630_06990.
PATRICi19439681. VBICloDif38397_0734.

Phylogenomic databases

eggNOGiCOG0016.
HOGENOMiHOG000242675.
KOiK01889.
OMAiMGKELNS.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630Imported.

Entry informationi

Entry nameiQ189P4_PEPD6
AccessioniPrimary (citable) accession number: Q189P4
Entry historyi
Integrated into UniProtKB/TrEMBL: July 25, 2006
Last sequence update: June 28, 2011
Last modified: March 4, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.