Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).SAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseSAAS annotationImported, Ligase

Keywords - Biological processi

Protein biosynthesisSAAS annotation

Keywords - Ligandi

ATP-bindingSAAS annotation, MagnesiumSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunitSAAS annotation (EC:6.1.1.20SAAS annotation)
Gene namesi
Name:pheSImported
Ordered Locus Names:CD630_06990Imported
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)Imported
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm SAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmSAAS annotation

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.SAAS annotation

Protein-protein interaction databases

STRINGi272563.CD0699.

Structurei

3D structure databases

ProteinModelPortaliQ189P4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 330220AA_TRNA_LIGASE_IIInterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili65 – 9228Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105CSS. Bacteria.
COG0016. LUCA.
HOGENOMiHOG000242675.
KOiK01889.
OMAiKGTGWLE.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q189P4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEKLLALRE AALAEIKEAQ SIESVESLRV KYLGKKGEIT AILKEMGKLS
60 70 80 90 100
AEERPVVGKV ANEVRENIEL SINSKKEEIN AIEKERKLKE EVIDVTQPGK
110 120 130 140 150
VLKVGKKHPI TQIIDEVTDI FIGMGFSIAE GPEVETVENN FDALNAPKDH
160 170 180 190 200
PSRDMSDTFY INDGVLLRTQ TSPVQVRTMR SQELPIKVIA PGRCFRSDSP
210 220 230 240 250
DATHSPMFHQ IEGLVVGKDV TMAEFKGTMD IFVEKLFGSD IKTKFRPHNF
260 270 280 290 300
PFTEPSAEVD VTCFKCGGKG CPMCKYEGWI EILGSGMVHP NVLRNCGIDP
310 320 330
EVYSGFAFGV GVERLAMLKY EIDDIRLLFE NDMRFLNQF
Length:339
Mass (Da):38,004
Last modified:June 28, 2011 - v2
Checksum:i16B501BEA3EE3C32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67532.2.
RefSeqiWP_003436795.1. NZ_CP010905.1.
YP_001087175.2. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67532; CAJ67532; CD630_06990.
GeneIDi4916807.
KEGGicdf:CD630_06990.
pdc:CDIF630_00814.
PATRICi19439681. VBICloDif38397_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67532.2.
RefSeqiWP_003436795.1. NZ_CP010905.1.
YP_001087175.2. NC_009089.1.

3D structure databases

ProteinModelPortaliQ189P4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67532; CAJ67532; CD630_06990.
GeneIDi4916807.
KEGGicdf:CD630_06990.
pdc:CDIF630_00814.
PATRICi19439681. VBICloDif38397_0734.

Phylogenomic databases

eggNOGiENOG4105CSS. Bacteria.
COG0016. LUCA.
HOGENOMiHOG000242675.
KOiK01889.
OMAiKGTGWLE.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630Imported.

Entry informationi

Entry nameiQ189P4_PEPD6
AccessioniPrimary (citable) accession number: Q189P4
Entry historyi
Integrated into UniProtKB/TrEMBL: July 25, 2006
Last sequence update: June 28, 2011
Last modified: May 11, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.