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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi333ZincUniRule annotation1
Metal bindingi384Zinc; via tele nitrogenUniRule annotation1
Metal bindingi509Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-685-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:CD630_05740
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000716751 – 639Threonine--tRNA ligaseAdd BLAST639

Proteomic databases

PRIDEiQ189B8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0574

Structurei

3D structure databases

ProteinModelPortaliQ189B8
SMRiQ189B8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni242 – 532CatalyticUniRule annotationAdd BLAST291

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C22 Bacteria
COG0441 LUCA
HOGENOMiHOG000003878
KOiK01868
OMAiDAHIFML

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.10.20.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR012675 Beta-grasp_dom_sf
IPR004095 TGS
IPR012676 TGS-like
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF02824 TGS, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
SSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q189B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVALKDGS IKEFENAISV MDVAKSISEG LARNVVAASV NGEVVGLDHI
60 70 80 90 100
IDTDCDLNLF KFEDKEGKEV FRHTSAHILA QAIKRLYPEA KLAIGPSIEN
110 120 130 140 150
GFYYDIDLDH RLVPEDLEKI EAEMKKIAKE DLKIERFELP RNEALELMKE
160 170 180 190 200
QGEDYKVELI SDLPESEIIS FYKQGDFTDL CRGPHLPSTK KVKAVKLQSV
210 220 230 240 250
AGAYWRGDEN NKMLQRIYGT SFEKNKDLEE YLHLLEEAKK RDHRKLGKEL
260 270 280 290 300
GLFMIPEEGP GFPMFLPKGM ELKNELLKFW REIHRKAGYI EIESPIILNR
310 320 330 340 350
KLWETSGHWY HYKENMYTVK IDDEDYAIKP MNCPGGLIYY NSQLHSYRDF
360 370 380 390 400
PMRVAELGRV HRHELSGALQ GLMRVRAFTQ DDSHIFMLPE QIKDEIKGVA
410 420 430 440 450
NLIDGIYKTF GFEYNLELST RPENSMGSDE EWEAAENGLR EALEELGLPY
460 470 480 490 500
TINEGDGAFY GPKIDFHLKD CLGRTWQCGT IQLDMQLPRQ FDNTYIGQDG
510 520 530 540 550
EKHRPVMIHR VAFGSIERFI GILIEHYAGK FPVWLSPTQV KILPISDKFM
560 570 580 590 600
DYANEVKKEL FDKGIRVELD DRAEKIGFKI REAQLEKVPY MLIVGEKEVA
610 620 630
DNNVSVRSRD KGEIGSIKLD EFIASISKEI ESRESIIQD
Length:639
Mass (Da):73,544
Last modified:July 25, 2006 - v1
Checksum:i4D9B4DA092C804A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ67406.1
RefSeqiWP_009888359.1, NZ_CP010905.2
YP_001087049.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ67406; CAJ67406; CD630_05740
GeneIDi31352128
4913359
KEGGicdf:CD630_05740
pdc:CDIF630_00687
PATRICifig|272563.120.peg.584

Similar proteinsi

Entry informationi

Entry nameiSYT_PEPD6
AccessioniPrimary (citable) accession number: Q189B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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