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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi333 – 3331Zinc; catalyticUniRule annotation
Metal bindingi384 – 3841Zinc; catalyticUniRule annotation
Metal bindingi509 – 5091Zinc; catalyticUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-707-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:CD630_05740
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639Threonine--tRNA ligasePRO_1000071675Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD0574.

Structurei

3D structure databases

ProteinModelPortaliQ189B8.
SMRiQ189B8. Positions 241-634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 532291CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C22. Bacteria.
COG0441. LUCA.
HOGENOMiHOG000003878.
KOiK01868.
OMAiYEMAIED.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR033728. ThrRS_core.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q189B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVALKDGS IKEFENAISV MDVAKSISEG LARNVVAASV NGEVVGLDHI
60 70 80 90 100
IDTDCDLNLF KFEDKEGKEV FRHTSAHILA QAIKRLYPEA KLAIGPSIEN
110 120 130 140 150
GFYYDIDLDH RLVPEDLEKI EAEMKKIAKE DLKIERFELP RNEALELMKE
160 170 180 190 200
QGEDYKVELI SDLPESEIIS FYKQGDFTDL CRGPHLPSTK KVKAVKLQSV
210 220 230 240 250
AGAYWRGDEN NKMLQRIYGT SFEKNKDLEE YLHLLEEAKK RDHRKLGKEL
260 270 280 290 300
GLFMIPEEGP GFPMFLPKGM ELKNELLKFW REIHRKAGYI EIESPIILNR
310 320 330 340 350
KLWETSGHWY HYKENMYTVK IDDEDYAIKP MNCPGGLIYY NSQLHSYRDF
360 370 380 390 400
PMRVAELGRV HRHELSGALQ GLMRVRAFTQ DDSHIFMLPE QIKDEIKGVA
410 420 430 440 450
NLIDGIYKTF GFEYNLELST RPENSMGSDE EWEAAENGLR EALEELGLPY
460 470 480 490 500
TINEGDGAFY GPKIDFHLKD CLGRTWQCGT IQLDMQLPRQ FDNTYIGQDG
510 520 530 540 550
EKHRPVMIHR VAFGSIERFI GILIEHYAGK FPVWLSPTQV KILPISDKFM
560 570 580 590 600
DYANEVKKEL FDKGIRVELD DRAEKIGFKI REAQLEKVPY MLIVGEKEVA
610 620 630
DNNVSVRSRD KGEIGSIKLD EFIASISKEI ESRESIIQD
Length:639
Mass (Da):73,544
Last modified:July 25, 2006 - v1
Checksum:i4D9B4DA092C804A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67406.1.
RefSeqiWP_009888359.1. NZ_CP010905.1.
YP_001087049.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ67406; CAJ67406; CD630_05740.
GeneIDi4913359.
KEGGicdf:CD630_05740.
pdc:CDIF630_00687.
PATRICi19439415. VBICloDif38397_0601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ67406.1.
RefSeqiWP_009888359.1. NZ_CP010905.1.
YP_001087049.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ189B8.
SMRiQ189B8. Positions 241-634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD0574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ67406; CAJ67406; CD630_05740.
GeneIDi4913359.
KEGGicdf:CD630_05740.
pdc:CDIF630_00687.
PATRICi19439415. VBICloDif38397_0601.

Phylogenomic databases

eggNOGiENOG4105C22. Bacteria.
COG0441. LUCA.
HOGENOMiHOG000003878.
KOiK01868.
OMAiYEMAIED.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-707-MONOMER.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR033728. ThrRS_core.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT_PEPD6
AccessioniPrimary (citable) accession number: Q189B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.