Q18973 (GSTK2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione s-transferase kappa 2
EC=2.5.1.18

Gene names

Name:	gstk-2
ORF Names:	D2024.7

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has roles in respiratory and lipid metabolism. Ref.2
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Mitochondrion Ref.2.
Tissue specificity	Expressed in the pharynx, body wall muscles and epidermis. Weaker expression is seen in the intestine. Ref.2
Disruption phenotype	Significant decrease in respiration rate and a lower concentration of the monounsaturated fatty acid cis-vaccenic acid. Ref.2
Sequence similarities	Belongs to the GST superfamily. Kappa family.

Ontologies

Keywords
Cellular component	Mitochondrion
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from direct assay Ref.2. Source: WormBase
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Glutathione s-transferase kappa 2		PRO_0000185894
Regions
	Region	15 – 17	3	Glutathione binding By similarity
	Region	200 – 201	2	Glutathione binding By similarity
Sites
	Binding site	52	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q18973 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 23D0A3D6762B7232	FASTA	225	25,951
	10 20 30 40 50 60 MPNRKVVKFF FDVISPYSYF GFEGITRHRS VWKTPIQMKP FFFAGVVRHT ENPGLPLRIP 70 80 90 100 110 120 IKEKYMHKDL LFSAQYWGIP FRLPKDYTNM MLNTSSIVPQ RILVASQLRD NVLMEDVARG 130 140 150 160 170 180 LWHRFYAYGK PIFTKSQVAE VLRDLHVKDV DELVMMSDSA EVKNILRENT DEAIGNGCFG 190 200 210 220 APWMHITDGH GKVLQTVFGS DRLPQVADFL AEPFKGPMRE KKPNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans." Petit E., Michelet X., Rauch C., Bertrand-Michel J., Terce F., Legouis R., Morel F. FEBS J. 276:5030-5040(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080386 Genomic DNA. Translation: CCD63360.1.
PIR	T34201.
RefSeq	NP_501148.1. NM_068747.4.
UniGene	Cel.13215.
3D structure databases
ProteinModelPortal	Q18973.
SMR	Q18973. Positions 2-218.
ModBase	Search...
Proteomic databases
PaxDb	Q18973.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	D2024.7.1; D2024.7.1; D2024.7. D2024.7.2; D2024.7.2; D2024.7.
GeneID	177498.
KEGG	cel:CELE_D2024.7.
Organism-specific databases
CTD	177498.
WormBase	D2024.7; CE04296; WBGene00017054; gstk-2.
Phylogenomic databases
eggNOG	COG3917.
GeneTree	ENSGT00440000033697.
HOGENOM	HOG000018051.
InParanoid	Q18973.
OMA	TLTLMRG.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR001853. DSBA-like_thioredoxin_dom. IPR014440. HCCAis_GSTk. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF01323. DSBA. 1 hit. [Graphical view]
PIRSF	PIRSF006386. HCCAis_GSTk. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
ProtoNet	Search...
Other
NextBio	897092.

Entry information

Entry name

GSTK2_CAEEL

Accession

Primary (citable) accession number: Q18973

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project