Reviewed, UniProtKB/Swiss-Prot Q188C8 (SYQ_CLOD6)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	CD2059

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095482
Regions
	Motif	33 – 43	11	"HIGH" region HAMAP MF_00126
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q188C8-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: E9AB923D5FAE0B4D	FASTA	554	64,281
	10 20 30 40 50 60 MSNETNSSNF IKNIIINDLE TGKHDSIITR FPPEPNGYLH IGHAKSICLN FGLAKEFNGK 70 80 90 100 110 120 ANLRFDDTNP LKEDVEYVES IKEDVKWLGF DWNELNFASN YFDEMYKRAL ILIKKGKAYV 130 140 150 160 170 180 CDLTQEEMRE YRGTLTEPGK ESPHRNRTIE ENLDLFERMK NGEFKDGEKT LRAKIDMSSP 190 200 210 220 230 240 NINLRDPIIY RISHSTHHNT GDKWCIYPMY AFAHPIEDAI EGITHSICTL EFEDQRPLYD 250 260 270 280 290 300 WFVKECEMEN IPRQIEFARL NINNTVMSKR KLKQLVDEGI VDGWDDPRVP TISGIRRKGY 310 320 330 340 350 360 TAEALRNFCS EIGVSKVNST VDSQMLDYFL RENLQPKAPL TMGVLRPLKL IITNYPEDKI 370 380 390 400 410 420 EMLEIENNAK DESQGKRLVP FSRELYIEQD DFMEEPVKKY FRFFPGNEVR LKGAYFVKCT 430 440 450 460 470 480 DVIKDENGNV VEIHGTYDPE TKSGSGFTGR KVKSTIHWVD AKSAIPCEFR LFEPLILDDI 490 500 510 520 530 540 PENEGKHFLE QINPNSLEIL QGFVEPTQIK DAKPFDKFQF VRNGFFSIDN KYTTDEKFVF 550 NRIVPLKSSF KPGK

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed: 16804543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM180355 Genomic DNA. Translation: CAJ68944.1.
RefSeq	YP_001088573.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q188C8.
Genome annotation databases
GeneID	4915499.
GenomeReviews	Gene locus CD2059 in contig AM180355_GR.
KEGG	cdf:CD2059.
NMPDR	fig|1496.1.peg.2720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q188C8.
OMA	RMPTIAG.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_CLOD6

Accession

Primary (citable) accession number: Q188C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)