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Q188C8 (SYQ_PEPD6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:CD630_20590
OrganismPeptoclostridium difficile (strain 630) (Clostridium difficile) [Complete proteome] [HAMAP]
Taxonomic identifier272563 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095482

Regions

Motif33 – 4311"HIGH" region HAMAP-Rule MF_00126
Motif266 – 2705"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q188C8 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: E9AB923D5FAE0B4D

FASTA55464,281
        10         20         30         40         50         60 
MSNETNSSNF IKNIIINDLE TGKHDSIITR FPPEPNGYLH IGHAKSICLN FGLAKEFNGK 

        70         80         90        100        110        120 
ANLRFDDTNP LKEDVEYVES IKEDVKWLGF DWNELNFASN YFDEMYKRAL ILIKKGKAYV 

       130        140        150        160        170        180 
CDLTQEEMRE YRGTLTEPGK ESPHRNRTIE ENLDLFERMK NGEFKDGEKT LRAKIDMSSP 

       190        200        210        220        230        240 
NINLRDPIIY RISHSTHHNT GDKWCIYPMY AFAHPIEDAI EGITHSICTL EFEDQRPLYD 

       250        260        270        280        290        300 
WFVKECEMEN IPRQIEFARL NINNTVMSKR KLKQLVDEGI VDGWDDPRVP TISGIRRKGY 

       310        320        330        340        350        360 
TAEALRNFCS EIGVSKVNST VDSQMLDYFL RENLQPKAPL TMGVLRPLKL IITNYPEDKI 

       370        380        390        400        410        420 
EMLEIENNAK DESQGKRLVP FSRELYIEQD DFMEEPVKKY FRFFPGNEVR LKGAYFVKCT 

       430        440        450        460        470        480 
DVIKDENGNV VEIHGTYDPE TKSGSGFTGR KVKSTIHWVD AKSAIPCEFR LFEPLILDDI 

       490        500        510        520        530        540 
PENEGKHFLE QINPNSLEIL QGFVEPTQIK DAKPFDKFQF VRNGFFSIDN KYTTDEKFVF 

       550 
NRIVPLKSSF KPGK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180355 Genomic DNA. Translation: CAJ68944.1.
RefSeqYP_001088573.1. NC_009089.1.

3D structure databases

ProteinModelPortalQ188C8.
SMRQ188C8. Positions 8-549.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272563.CD2059.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ68944; CAJ68944; CD630_20590.
GeneID4915499.
KEGGcdf:CD630_20590.
PATRIC19442559. VBICloDif38397_2166.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMASREIYID.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycCDIF272563:GJFE-2267-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_PEPD6
AccessionPrimary (citable) accession number: Q188C8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries