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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei269 – 2691ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: InterPro
  2. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:CD630_20590
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
ProteomesiUP000001978 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Glutamine--tRNA ligasePRO_1000095482Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD2059.

Structurei

3D structure databases

ProteinModelPortaliQ188C8.
SMRiQ188C8. Positions 8-549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi33 – 4311"HIGH" regionAdd
BLAST
Motifi266 – 2705"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiVTHSICT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q188C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNETNSSNF IKNIIINDLE TGKHDSIITR FPPEPNGYLH IGHAKSICLN
60 70 80 90 100
FGLAKEFNGK ANLRFDDTNP LKEDVEYVES IKEDVKWLGF DWNELNFASN
110 120 130 140 150
YFDEMYKRAL ILIKKGKAYV CDLTQEEMRE YRGTLTEPGK ESPHRNRTIE
160 170 180 190 200
ENLDLFERMK NGEFKDGEKT LRAKIDMSSP NINLRDPIIY RISHSTHHNT
210 220 230 240 250
GDKWCIYPMY AFAHPIEDAI EGITHSICTL EFEDQRPLYD WFVKECEMEN
260 270 280 290 300
IPRQIEFARL NINNTVMSKR KLKQLVDEGI VDGWDDPRVP TISGIRRKGY
310 320 330 340 350
TAEALRNFCS EIGVSKVNST VDSQMLDYFL RENLQPKAPL TMGVLRPLKL
360 370 380 390 400
IITNYPEDKI EMLEIENNAK DESQGKRLVP FSRELYIEQD DFMEEPVKKY
410 420 430 440 450
FRFFPGNEVR LKGAYFVKCT DVIKDENGNV VEIHGTYDPE TKSGSGFTGR
460 470 480 490 500
KVKSTIHWVD AKSAIPCEFR LFEPLILDDI PENEGKHFLE QINPNSLEIL
510 520 530 540 550
QGFVEPTQIK DAKPFDKFQF VRNGFFSIDN KYTTDEKFVF NRIVPLKSSF

KPGK
Length:554
Mass (Da):64,281
Last modified:July 25, 2006 - v1
Checksum:iE9AB923D5FAE0B4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68944.1.
RefSeqiYP_001088573.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ68944; CAJ68944; CD630_20590.
GeneIDi4915499.
KEGGicdf:CD630_20590.
PATRICi19442559. VBICloDif38397_2166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68944.1.
RefSeqiYP_001088573.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ188C8.
SMRiQ188C8. Positions 8-549.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD2059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68944; CAJ68944; CD630_20590.
GeneIDi4915499.
KEGGicdf:CD630_20590.
PATRICi19442559. VBICloDif38397_2166.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiVTHSICT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2267-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630.

Entry informationi

Entry nameiSYQ_PEPD6
AccessioniPrimary (citable) accession number: Q188C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 25, 2006
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.