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Q18825

- BAR1_CAEEL

UniProt

Q18825 - BAR1_CAEEL

Protein

Beta-catenin/armadillo-related protein 1

Gene

bar-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Participates in the Wnt signaling pathway which affects cell fate and may regulate the stem cell divisions of seam cells during larval development. Functions as a transcriptional activator but is dependent on the interaction with pop-1. Involved in maintaining lin-39 Hox expression and regulating glr-1 abundance at the synapses. Required for mab-5 expression during Q neuroblast migration and for oxidative stress-induced daf-16 signaling. Has roles in egg laying, vulva precursor cell fate determination, Q neuroblast migration, posterior ectodermal cell P12 specification, movement, body length, male tail development and dauer induction. Functionally redundant to wrm-1 and hmp-2.8 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. transcription coactivator activity Source: WormBase
    3. transcription factor binding Source: WormBase

    GO - Biological processi

    1. cell adhesion Source: UniProtKB
    2. cell fate specification Source: UniProtKB
    3. hermaphrodite genitalia development Source: WormBase
    4. mating behavior Source: WormBase
    5. morphogenesis of an epithelium Source: WormBase
    6. oviposition Source: WormBase
    7. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
    8. positive regulation of vulval development Source: WormBase
    9. Ras protein signal transduction Source: WormBase
    10. regulation of backward locomotion Source: WormBase
    11. regulation of cell fate specification Source: WormBase
    12. regulation of cell migration Source: WormBase
    13. regulation of vulval development Source: WormBase
    14. transcription, DNA-templated Source: UniProtKB-KW
    15. Wnt signaling pathway Source: WormBase

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation, Wnt signaling pathway

    Enzyme and pathway databases

    SignaLinkiQ18825.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-catenin/armadillo-related protein 1
    Alternative name(s):
    Protruding vulva protein 1
    Suppressor of polyray 1
    Gene namesi
    Name:bar-1Imported
    Synonyms:pvl-1, spy-1
    ORF Names:C54D1.6
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiC54D1.6; CE08973; WBGene00000238; bar-1.

    Subcellular locationi

    Cytoplasm 2 Publications. Nucleus 2 Publications. Membrane 2 Publications. Cell junction 2 Publications
    Note: Mostly cytoplasmic.2 Publications

    GO - Cellular componenti

    1. cell-cell adherens junction Source: WormBase
    2. cell junction Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. cytosol Source: WormBase
    5. membrane Source: WormBase
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi239 – 2391S → L in ep451; interferes with neuroblast Q migration. 1 Publication
    Mutagenesisi278 – 2781V → G in ep484; interferes with neuroblast Q migration; in association with Asn-281. 1 Publication
    Mutagenesisi281 – 2811D → N in ep484; interferes with neuroblast Q migration; in association with Gly-278. 1 Publication
    Mutagenesisi369 – 3691S → K in ep466; interferes with neuroblast Q migration. 1 Publication
    Mutagenesisi449 – 4491E → K in ep487/ep478; variable interference with neuroblast Q migration. 1 Publication
    Mutagenesisi460 – 4601R → W in ep461; interferes with neuroblast Q migration. 1 Publication
    Mutagenesisi482 – 4821L → I in mu349; suppresses ectopic expression of mab-5 and interferes with neuroblast Q migration. 2 Publications
    Mutagenesisi498 – 4981T → I in ep485; interferes with neuroblast Q migration. 1 Publication
    Mutagenesisi524 – 5241G → D in ep460/mu63; suppresses ectopic expression of mab-5. 2 Publications
    Mutagenesisi524 – 5241G → S in ep486; interferes with neuroblast Q migration. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 811811Beta-catenin/armadillo-related protein 1PRO_0000372805Add
    BLAST

    Proteomic databases

    PaxDbiQ18825.
    PRIDEiQ18825.

    Expressioni

    Tissue specificityi

    Expressed in body wall muscle cells, hypodermal seam cells, gonad sheath cells and vulval precursor cells.2 Publications

    Developmental stagei

    Detected throughout development from L1 to L4 and during adulthood. Detected from the P3.p cell stage through to P8.p.2 Publications

    Interactioni

    Subunit structurei

    Interacts with apr-1, axl-1, daf-16, lin-23, and pop-1 (via acidic region in N-terminus 1-44). Interacts (via ARM repeats) with pry-1.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pop-1Q106665EBI-2528850,EBI-317870
    pry-1O620903EBI-2528850,EBI-2917690

    Protein-protein interaction databases

    BioGridi45907. 184 interactions.
    IntActiQ18825. 4 interactions.
    MINTiMINT-214589.
    STRINGi6239.C54D1.6.2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ18825.
    SMRiQ18825. Positions 109-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati108 – 14740ARM 1Sequence AnalysisAdd
    BLAST
    Repeati331 – 36939ARM 2Sequence AnalysisAdd
    BLAST
    Repeati370 – 40839ARM 3Sequence AnalysisAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8585Involved in transcriptional activation1 PublicationAdd
    BLAST
    Regioni541 – 811271Involved in transcriptional activation1 PublicationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the beta-catenin family.Sequence Analysis
    Contains 3 ARM repeats.Sequence Analysis

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG249506.
    GeneTreeiENSGT00730000110821.
    HOGENOMiHOG000095175.
    InParanoidiQ18825.
    KOiK02105.
    OMAiNIAKMEC.
    OrthoDBiEOG7X0VMV.
    PhylomeDBiQ18825.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    IPR013284. Beta-catenin.
    [Graphical view]
    PRINTSiPR01869. BCATNINFAMLY.
    SMARTiSM00185. ARM. 3 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q18825-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLDPNLVIN HDDTNLSEAS FTMEQHTSSY SDIHMGSTPC TGHRKVDMWR    50
    NHNFDSGFQT MNHSEAPSII SSLHPSSHLS GMSSMADYEP IPTLSDQQKQ 100
    KFDGITQNQA DGQYNTVRAI PELTMLMKDQ DNEVVHKAVI LMQNIAKMEC 150
    DPMRRQNEAR IVDPRVIFTL RDLLRDKVEF PNIIRCTLGT FFHICNRQEG 200
    IDLVTRAIAE QPDIIPNLIR HIGTYPSSIY KYAILTMHSI LSDKQRGGQS 250
    VIIARQQDAI THVTPWLEAE KSEKLLPVIV DLIRVLCEKN TEQKIKFVKM 300
    GGPQKLLMLL QHRVYENLLW RTTQLLKTFS NFDAPNLVAF GGRQILANLL 350
    SHGSPRLVQS TLETLRNISD VPSKIKEDLL LKSLLELVNS RNTTIRLYSA 400
    QIMSNLVANN RHNKEFMCGN NGVVILVRAL TIATKEMGDL RDKEAQQMED 450
    YIESLICTLR HLCVGHPMSD KVQAFVFRDP ALFLHKLLTM RPVLLKHTLS 500
    LLLKVVSQHA LLAPFRSCRI GDKGFVEQLI HILRVACTQL NVQESIEGVR 550
    VKDIIHLCIQ ILRWITRDQD ILNEVVFFLQ TPENSRMGDG HTLPIFVLQK 600
    ANVEENTKSS ALALIYNLMH HEQMANVLDR DDVLVKMLQN VQMQSQTHPE 650
    LASLANNILK MMYEKREKTR NTLPRYNSYL ESQFGHMSMT TPRSEALNSS 700
    GEVCEGAGEQ WSTPLTDDTM MDSYCNSSGR DSSKPYNSPM YHSPPAMYPE 750
    YSIGPPETYL DPHATASCYP RPTPPQYNSY DRSPPVYNDL PSNPGPSSHS 800
    SDYYPSRNSR F 811
    Length:811
    Mass (Da):92,228
    Last modified:June 1, 1998 - v2
    Checksum:i97D6FFDE71BDFDFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF063646 mRNA. Translation: AAC17424.1.
    FO080795 Genomic DNA. Translation: CCD66852.1.
    PIRiT43175.
    RefSeqiNP_509206.1. NM_076805.5.
    UniGeneiCel.5480.

    Genome annotation databases

    EnsemblMetazoaiC54D1.6; C54D1.6; WBGene00000238.
    GeneIDi180982.
    KEGGicel:CELE_C54D1.6.
    UCSCiC54D1.6.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF063646 mRNA. Translation: AAC17424.1 .
    FO080795 Genomic DNA. Translation: CCD66852.1 .
    PIRi T43175.
    RefSeqi NP_509206.1. NM_076805.5.
    UniGenei Cel.5480.

    3D structure databases

    ProteinModelPortali Q18825.
    SMRi Q18825. Positions 109-678.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 45907. 184 interactions.
    IntActi Q18825. 4 interactions.
    MINTi MINT-214589.
    STRINGi 6239.C54D1.6.2.

    Proteomic databases

    PaxDbi Q18825.
    PRIDEi Q18825.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C54D1.6 ; C54D1.6 ; WBGene00000238 .
    GeneIDi 180982.
    KEGGi cel:CELE_C54D1.6.
    UCSCi C54D1.6.1. c. elegans.

    Organism-specific databases

    CTDi 180982.
    WormBasei C54D1.6 ; CE08973 ; WBGene00000238 ; bar-1.

    Phylogenomic databases

    eggNOGi NOG249506.
    GeneTreei ENSGT00730000110821.
    HOGENOMi HOG000095175.
    InParanoidi Q18825.
    KOi K02105.
    OMAi NIAKMEC.
    OrthoDBi EOG7X0VMV.
    PhylomeDBi Q18825.

    Enzyme and pathway databases

    SignaLinki Q18825.

    Miscellaneous databases

    NextBioi 911834.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    IPR013284. Beta-catenin.
    [Graphical view ]
    PRINTSi PR01869. BCATNINFAMLY.
    SMARTi SM00185. ARM. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The beta-catenin homolog BAR-1 and LET-60 Ras coordinately regulate the Hox gene lin-39 during Caenorhabditis elegans vulval development."
      Eisenmann D.M., Maloof J.N., Simske J.S., Kenyon C., Kim S.K.
      Development 125:3667-3680(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Bristol N2Imported.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "A Wnt signaling pathway controls hox gene expression and neuroblast migration in C. elegans."
      Maloof J.N., Whangbo J., Harris J.M., Jongeward G.D., Kenyon C.
      Development 126:37-49(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LEU-482 AND GLY-524.
    4. "Protruding vulva mutants identify novel loci and Wnt signaling factors that function during Caenorhabditis elegans vulva development."
      Eisenmann D.M., Kim S.K.
      Genetics 156:1097-1116(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Distinct beta-catenins mediate adhesion and signalling functions in C. elegans."
      Korswagen H.C., Herman M.A., Clevers H.C.
      Nature 406:527-532(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH POP-1.
    6. "The divergent Caenorhabditis elegans beta-catenin proteins BAR-1, WRM-1 and HMP-2 make distinct protein interactions but retain functional redundancy in vivo."
      Natarajan L., Witwer N.E., Eisenmann D.M.
      Genetics 159:159-172(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH APR-1 AND POP-1.
    7. "The axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans."
      Korswagen H.C., Coudreuse D.Y.M., Betist M.C., van de Water S., Zivkovic D., Clevers H.C.
      Genes Dev. 16:1291-1302(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRY-1.
    8. "A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification in C. elegans."
      Moghal N., Sternberg P.W.
      Development 130:57-69(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Identification of evolutionarily conserved promoter elements and amino acids required for function of the C. elegans beta-catenin homolog BAR-1."
      Natarajan L., Jackson B.M., Szyleyko E., Eisenmann D.M.
      Dev. Biol. 272:536-557(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-239; VAL-278; ASP-281; SER-369; GLU-449; ARG-460; LEU-482; THR-498 AND GLY-524.
    10. "LIN-23-mediated degradation of beta-catenin regulates the abundance of GLR-1 glutamate receptors in the ventral nerve cord of C. elegans."
      Dreier L., Burbea M., Kaplan J.M.
      Neuron 46:51-64(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LIN-23.
    11. "Functional interaction between beta-catenin and FOXO in oxidative stress signaling."
      Essers M.A.G., de Vries-Smits L.M.M., Barker N., Polderman P.E., Burgering B.M.T., Korswagen H.C.
      Science 308:1181-1184(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH DAF-16.
    12. "Two functionally distinct axin-like proteins regulate canonical Wnt signaling in C. elegans."
      Oosterveen T., Coudreuse D.Y.M., Yang P.-T., Fraser E., Bergsma J., Dale T.C., Korswagen H.C.
      Dev. Biol. 308:438-448(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AXL-1.

    Entry informationi

    Entry nameiBAR1_CAEEL
    AccessioniPrimary (citable) accession number: Q18825
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Miscellaneous

    Worms lacking bar-1 exhibit abnormalities in vulval development due to incorrect cell fate specification, resulting in the following phenotypes, multivulva (Muv), protruding vulva (Pvl) and egg laying defective (Egl). Mutants also show defects in Q neuroblast migration, uncoordinated movement (Unc) and reduced superoxidase dismutase levels. Null mutants can be rescued by wrm-1 and hmp-2 when expressed from the bar-1 promoter. Double mutants also lacking dpy-22 show reduced body length, male tail morphology abnormalities (Mab) and dauer induction.5 Publications

    Caution

    Alleles mu63 and mu349 were originally reported (PubMed:9834184) as the mutations L130F and Q147STOP respectively. This is in conflict with WormBase and more recent literature (PubMed:15282167) which is represented in this entry.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3