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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi84Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi118Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi194Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi198Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi201Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-2127-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:CD630_19860
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003742281 – 478tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST478

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD1986

Structurei

3D structure databases

ProteinModelPortaliQ187U6
SMRiQ187U6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 157MTTase N-terminalUniRule annotationAdd BLAST119
Domaini410 – 477TRAMUniRule annotationAdd BLAST68

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW Bacteria
COG0621 LUCA
HOGENOMiHOG000224767
KOiK06168
OMAiKVCEHFH

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q187U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRKQVTIP IEKIQEQDKY IEQIHRQNEE YFNRTGKRKL VFTQTFGCQM
60 70 80 90 100
NEHDSEKLCS MLEEMGYQMS MMVEESDLII YNTCAVRENA ELKVYGNLGQ
110 120 130 140 150
LKHLKGKNPD MKIAVCGCMM QQPHVVEELR KKYKHVDLIF GTHNLYKFPQ
160 170 180 190 200
LLTESINSDK MLVDVWDVDG EVIEGLRSNR KFELKAFVNI MYGCNNFCTY
210 220 230 240 250
CIVPYTRGRE RSRTPEDIIN EIKELVANGT KEITLLGQNV DSYGKTLENP
260 270 280 290 300
VSFSELLRKV NDIEGIERVR FMTSHPKDIS DEVIYAIRDC DKVCEFLHLP
310 320 330 340 350
IQCGSSSLLK KMNRHYTKEY YLEIIEKAKK EVPGIAFSTD LMIGFPGETE
360 370 380 390 400
EDLLDTLDVV EKVRYDSAFT FIYSKRQGTP AAKMENQIPE DIKHDRFNRV
410 420 430 440 450
LEAVNRISAE INDGYKDRIV EVLVEGRSKN NENKFAGRTR QNKLVNFEGG
460 470
NDDLIGKLVM VKITEPRTFS LNGILVNN
Length:478
Mass (Da):55,275
Last modified:July 25, 2006 - v1
Checksum:i9E0E4D957ECC3963
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ68861.1
RefSeqiWP_003435252.1, NZ_CP010905.2
YP_001088491.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ68861; CAJ68861; CD630_19860
GeneIDi31353563
4913654
KEGGicdf:CD630_19860
pdc:CDIF630_02195
PATRICifig|272563.120.peg.2085

Similar proteinsi

Entry informationi

Entry nameiMIAB_PEPD6
AccessioniPrimary (citable) accession number: Q187U6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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