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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi84 – 841Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi118 – 1181Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi194 – 1941Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi198 – 1981Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi201 – 2011Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:CD630_19860
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374228Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD1986.

Structurei

3D structure databases

ProteinModelPortaliQ187U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 157119MTTase N-terminalUniRule annotationAdd
BLAST
Domaini410 – 47768TRAMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q187U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRKQVTIP IEKIQEQDKY IEQIHRQNEE YFNRTGKRKL VFTQTFGCQM
60 70 80 90 100
NEHDSEKLCS MLEEMGYQMS MMVEESDLII YNTCAVRENA ELKVYGNLGQ
110 120 130 140 150
LKHLKGKNPD MKIAVCGCMM QQPHVVEELR KKYKHVDLIF GTHNLYKFPQ
160 170 180 190 200
LLTESINSDK MLVDVWDVDG EVIEGLRSNR KFELKAFVNI MYGCNNFCTY
210 220 230 240 250
CIVPYTRGRE RSRTPEDIIN EIKELVANGT KEITLLGQNV DSYGKTLENP
260 270 280 290 300
VSFSELLRKV NDIEGIERVR FMTSHPKDIS DEVIYAIRDC DKVCEFLHLP
310 320 330 340 350
IQCGSSSLLK KMNRHYTKEY YLEIIEKAKK EVPGIAFSTD LMIGFPGETE
360 370 380 390 400
EDLLDTLDVV EKVRYDSAFT FIYSKRQGTP AAKMENQIPE DIKHDRFNRV
410 420 430 440 450
LEAVNRISAE INDGYKDRIV EVLVEGRSKN NENKFAGRTR QNKLVNFEGG
460 470
NDDLIGKLVM VKITEPRTFS LNGILVNN
Length:478
Mass (Da):55,275
Last modified:July 25, 2006 - v1
Checksum:i9E0E4D957ECC3963
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68861.1.
RefSeqiWP_003435252.1. NZ_CP010905.1.
YP_001088491.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ68861; CAJ68861; CD630_19860.
GeneIDi4913654.
KEGGicdf:CD630_19860.
pdc:CDIF630_02195.
PATRICi19442393. VBICloDif38397_2083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68861.1.
RefSeqiWP_003435252.1. NZ_CP010905.1.
YP_001088491.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ187U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68861; CAJ68861; CD630_19860.
GeneIDi4913654.
KEGGicdf:CD630_19860.
pdc:CDIF630_02195.
PATRICi19442393. VBICloDif38397_2083.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiEOG6P5ZD8.

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2181-MONOMER.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 630.

Entry informationi

Entry nameiMIAB_PEPD6
AccessioniPrimary (citable) accession number: Q187U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 25, 2006
Last modified: November 11, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.