Q187Q1 (TRMB_CLOD6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	CD630_19420

Organism

Clostridium difficile (strain 630) [Complete proteome] [HAMAP]

Taxonomic identifier

272563 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Peptostreptococcaceae › Clostridium difficile

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000288138
Regions
	Region	192 – 195	4	Substrate binding Potential
Sites
	Binding site	45	1	S-adenosyl-L-methionine By similarity
	Binding site	70	1	S-adenosyl-L-methionine By similarity
	Binding site	97	1	S-adenosyl-L-methionine By similarity
	Binding site	119	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	155	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q187Q1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: B4FCF6EE80BDC26C	FASTA	214	25,378
	10 20 30 40 50 60 MRRRRKKGAD EKLLSYTKYV LRDDIDKLKG KWNLKFRNDN PIHVEFGTGK GKFITTLAKQ 70 80 90 100 110 120 NPDINYIAME LKEEVLLKAV EKADASNLNN ILFLWGDVSN ILDYFEAKEL SRIYINFCDP 130 140 150 160 170 180 WPKNRWSKRR LTHSGFLEMY NRVLEDDGEI HFKTDNEKLF EFSLNEIAAN NWLLKNISLD 190 200 210 LGNSEYENNV TTEYEDKFMS QGMRIFRCEA KKRN

References

[1]

"The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome." Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H., Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N., Brooks K., Chillingworth T. , Cronin A., Davis P., Dowd L., Fraser A., Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K., Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L., Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J. Nat. Genet. 38:779-786(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180355 Genomic DNA. Translation: CAJ68817.2.
RefSeq	YP_001088448.2. NC_009089.1.
3D structure databases
ProteinModelPortal	Q187Q1.
ModBase	Search...
Protein-protein interaction databases
STRING	272563.CD1942.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ68817; CAJ68817; CD630_19420.
GeneID	4913902.
KEGG	cdf:CD630_19420.
PATRIC	19442303. VBICloDif38397_2038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000251689.
ProtClustDB	CLSK2535076.
Enzyme and pathway databases
BioCyc	CDIF272563:GJFE-2135-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CLOD6

Accession

Primary (citable) accession number: Q187Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program