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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE1), Shikimate dehydrogenase (NADP(+)) (aroE2)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261Shikimate-3-phosphateUniRule annotation
Binding sitei129 – 1291PhosphoenolpyruvateUniRule annotation
Binding sitei200 – 2001Shikimate-3-phosphateUniRule annotation
Active sitei314 – 3141Proton acceptorUniRule annotation
Binding sitei341 – 3411Shikimate-3-phosphateUniRule annotation
Active sitei342 – 3421Proton donorUniRule annotation
Binding sitei345 – 3451PhosphoenolpyruvateUniRule annotation
Binding sitei388 – 3881PhosphoenolpyruvateUniRule annotation
Binding sitei414 – 4141PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciCDIF272563:GJFE-2025-MONOMER.
UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:CD630_18340
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4374373-phosphoshikimate 1-carboxyvinyltransferasePRO_1000099688Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD1834.

Structurei

3D structure databases

ProteinModelPortaliQ187E3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 222Shikimate-3-phosphate bindingUniRule annotation
Regioni99 – 1024PhosphoenolpyruvateUniRule annotation
Regioni172 – 1743Shikimate-3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247372.
KOiK00800.
OMAiKSYPDFW.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q187E3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLKIYPSK LSGDVKIPPS KSMAHRAVIC SSLSNGKSRI SNIDFSDDII
60 70 80 90 100
ATIRAMTSLG AIIEKKEDIL EISGIFSKEG ILNRENQLNQ PKLTIDCNES
110 120 130 140 150
GSTLRFLVPI SLAFDGVKRF IGRGNLGKRP LDTYYEIFDR QNIKYSYKEN
160 170 180 190 200
QLDLIISGKL KPDEFRVKGN ISSQFITGLL FILPTLESDS KIIITTELES
210 220 230 240 250
KGYLDLTLST IKDFGVEIIN NNYKEFIIKG NQTYKARDYK VEGDYSQGAF
260 270 280 290 300
YLSADAIGED ISILDLKEDS LQGDSEVVEI LSRMGMEILR EGNKIKGITN
310 320 330 340 350
GLNGTLIDAS QCPDIIPVLS VVASLSIGKT TIINAGRLRI KECDRLHAIN
360 370 380 390 400
VELSKLGANI EEKEDSLIIE GVSKLNGGVE VWSHKDHRIA MTLAIASCRC
410 420 430
DKPIILKDFE CVSKSYPHFF KDFKMLGGRI DEWNMGK
Length:437
Mass (Da):48,581
Last modified:July 25, 2006 - v1
Checksum:i1FAE0AE919D6968A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68705.1.
RefSeqiWP_011861345.1. NZ_CP010905.1.
YP_001088340.1. NC_009089.1.

Genome annotation databases

EnsemblBacteriaiCAJ68705; CAJ68705; CD630_18340.
GeneIDi4914602.
KEGGicdf:CD630_18340.
pdc:CDIF630_02036.
PATRICi19442077. VBICloDif38397_1925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA. Translation: CAJ68705.1.
RefSeqiWP_011861345.1. NZ_CP010905.1.
YP_001088340.1. NC_009089.1.

3D structure databases

ProteinModelPortaliQ187E3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68705; CAJ68705; CD630_18340.
GeneIDi4914602.
KEGGicdf:CD630_18340.
pdc:CDIF630_02036.
PATRICi19442077. VBICloDif38397_1925.

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247372.
KOiK00800.
OMAiKSYPDFW.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.
BioCyciCDIF272563:GJFE-2025-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROA_PEPD6
AccessioniPrimary (citable) accession number: Q187E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.